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001-es BibID:	BIBFORM042646
Első szerző:	Nedvig Klára
Cím:	Changes of PACAP immunoreactivities and cytokine levels after PACAP-38 containing intestinal preservation and autotransplantation / Klara Nedvig, Gyorgy Weber, Jozsef Nemeth, Krisztina Kovacs, Dora Reglodi, Agnes Kemeny, Andrea Ferencz
Dátum:	2012
Megjegyzések:	Small bowel is one of the most sensitive organs toischemia?reperfusion injury, which is a significant problemduring transplantation. Pituitary adenylate cyclaseactivatingpolypeptide (PACAP) has cytoprotective effect inischemic injuries of various tissues. The aim of our study wasto measure changes of PACAP-38 and PACAP-27 immunoreactivitiesand cytokine levels in intestinal grafts stored inPACAP-38-containing preservation solution. Small bowelautotransplantation was performed on maleWistar rats. Graftswere stored in University ofWisconsin (UW) solution at 4 ?Cfor 1 h (group (G)I), for 3 h (GII), and for 6 h (GIII) and inPACAP-38-containing UW solution for 1 h (GIV), for 3 h(GV), and for 6 h (GVI).After preservation, performing vesselanastomosis reperfusion began, which lasted 3 h in eachgroup. Tissue biopsies were collected after laparotomy(control) and at the end of the reperfusion periods. IntestinalPACAP-38 and PACAP-27 immunoreactivities were measuredby radioimmunoassay. To measure cytokines from tissuehomogenates, we used rat cytokine array and LuminexMultiplex Immunoassay. Levels of PACAP-38 and PACAP-27 immunoreactivity decreased after 1 and 3 h preservationcompared to control levels. This decrease was significantfollowing 6 h cold storage (p<0.05). Values remained significantlyhigher in grafts stored in PACAP-38-containing UW.Cytokine array revealed that expression of the soluble intercellularadhesion molecule-1 (CD54) and L-selectin (CD62L/LECAM-1) was increased in GIII. Both 6 h cold storage inPACAP-38-containing UW solution and 3 h reperfusioncaused strong reduction in these cytokines activation in GVI.RANTES (CCL5) levels were increased in all groups. Strongactivation of the tissue inhibitor of metalloproteinase-1 was inGIII. However, PACAP-38-containing cold storage could decreaseits activation in GVI. Furthermore, strong activation ofthe tissue inhibitor of metalloproteinase-1 was detected in 6 hpreserved grafts without PACAP-38 (GIII). PACAP-38-containing cold storage could decrease its activation in GVI.Our present study showed that PACAP-38 and PACAP-27immunoreactivities decreased in a time-dependent mannerduring intestinal cold preservation, which could be amelioratedby administration of exogenous PACAP-38 to the preservationsolution. Moreover, PACAP-38 could attenuate tissuecold ischemic injury-induced changes in cytokine expression.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Small bowel Transplantation PACAP-38 PACAP-27 Cytokine
Megjelenés:	Journal of Molecular Neuroscience. - 48 : 3 (2012), p. 788-794. -
További szerzők:	Wéber György Németh József (1954-) (vegyész, analitikus) Kovács Krisztina (Pécs) Reglődi Dóra (Idegtudományok) Kemény Ágnes Ferencz Andrea
Pályázati támogatás:	OTKA-77474 OTKA
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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