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001-es BibID:BIBFORM047048
035-os BibID:PMID:7667292
Első szerző:Pitcher, Julie A.
Cím:The G-protein-coupled receptor phosphatase: a protein phosphatase type 2A with a distinct subcellular distribution and substrate specificity / Julie A. Pitcher, E. Sturgis Payne, Csilla Csortos, Anna A. DePaoli-Roach, Robert J. Lefkowitz
Dátum:1995
ISSN:0027-8424
Megjegyzések:Phosphorylation of G-protein-coupled receptors plays an important role in regulating their function. In this study the G-protein-coupled receptor phosphatase (GRP) capable of dephosphorylating G-protein-coupled receptor kinase-phosphorylated receptors is described. The GRP activity of bovine brain is a latent oligomeric form of protein phosphatase type 2A (PP-2A) exclusively associated with the particulate fraction. GRP activity is observed only when assayed in the presence of protamine or when phosphatase-containing fractions are subjected to freeze/thaw treatment under reducing conditions. Consistent with its identification as a member of the PP-2A family, the GRP is potently inhibited by okadaic acid but not by I-2, the specific inhibitor of protein phosphatase type 1. Solubilization of the membrane-associated GRP followed by gel filtration in the absence of detergent yields a 150-kDa peak of latent receptor phosphatase activity. Western blot analysis of this phosphatase reveals a likely subunit composition of AB alpha C. PP-2A of this subunit composition has previously been characterized as a soluble enzyme, yet negligible soluble GRP activity was observed. The subcellular distribution and substrate specificity of the GRP suggests significant differences between it and previously characterized forms of PP-2A.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Proceedings of the National Academy of Sciences of The United States of America. - 92 : 18 (1995), p. 8343-8347. -
További szerzők:Payne, E. Sturgis Csortos Csilla (1956-) (biokémikus) DePaoli-Roach, Anna A. Lefkowitz, Robert J.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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