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001-es BibID:BIBFORM048516
Első szerző:Saha, Debjani
Cím:Simultaneous enzyme immunoassay for the screening of aflatoxin B1 and ochratoxin A in chili samples / Debjani Saha, Debopam Acharya, Dipika Roy, Dilip Shrestha, Tarun K. Dhar
Dátum:2007
Megjegyzések:Membrane-based immunoassay has been developed for simultaneous estimation of aflatoxin B(1) (AFB(1)) and ochratoxin A (OA) in chili samples. The combined estimation of both the mycotoxins is more economical in respect of time, work and materials than two separate assays. The method uses a low cost test device consisting of a membrane with immobilized anti-AFB(1) and anti-OA antibodies and a filter paper attached to a polyethylene card below the membrane. It allows direct analysis of sample extracts containing substantial amount (40%) of methanol. This permits the use of two-fold diluted sample extracts resulting in minimum dilution error. The limit of quantitation obtained was 2 and 10 microg kg(-1) for AFB(1) and OA, respectively. The tolerance of 40% methanol was found to be due to the application of small size (0.8 mm diameter) spots on membranes, as the tolerance decreases to 20% with gradual increase in spot size. The combined method is capable of producing acceptable results to analyze AFB(1) and OA in chili with accuracy and precision. The AFB(1) and OA values obtained for spiked and naturally contaminated chili samples by the simultaneous method were in good correlation with those measured by individual ELISA. The method offers a simple, rapid and cost-effective screening tool to meet the requirements of the rapidly evolving EU legislation
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Aflatoxin B1
analysis
Antibodies
article
Capsicum
chemistry
Food Contamination
Immunoenzyme Techniques
immunology
Ochratoxins
Plant Extracts
Research
Research Support
Support
time
Megjelenés:Analytica Chimica Acta. - 584 : 2 (2007), p. 343-349. -
További szerzők:Acharya, Debopam Roy, Dipika Shrestha, Dilip (1980-) (biológus) Dhar, Tarun K.
Internet cím:DOI
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