Összesen 1 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM054348
035-os BibID:PMID: 7843241
Első szerző:Sármay Gabriella
Cím:The alternative splicing of human FcγRII mRNA is regulated by activation of B cells with mIgM cross-linking, interleukin-4, or phorbolester / Gabriella Sármay, Zoltán Rozsnyay, Gábor Koncz, Alla Danilkovich, János Gergely
Dátum:1995
ISSN:0014-2980
Megjegyzések:The human type two IgG binding receptors (Fc gamma RII) are encoded by three genes (Fc gamma RIIA, -B and C) resulting in at least six protein isoforms generated by alternative mRNA splicing. Surface expression of Fc gamma RTT has been shown to be modulated during B cell activation, although data characterizing the isoform(s) expressed are not available. The extracellular as well as the transmembrane domains of various Fc gamma RII are highly homologous. Only the intracellular domains vary between the different Fc gamma RII isoforms, suggesting differences in signal transduction. Using reverse transcriptase and polymerase chain reaction of mRNA obtained from resting tonsil B cells,we show that the majority of Fc gamma RII mRNA species to be of b2 type, although b1 type and a low level of Fc gamma RIIa type are also present. Culturing the cells for 18 h in the presence of 2.5 U/ml interleukin-4 or 10 mu g/ml affinity-purified anti-IgM F(ab')(2) fragments induced a switch in alternative splicing, resulting in a significant increase of Fc gamma RIIb1 mRNA expression, while the synthesis of Fc gamma RIIb2 mRNA was down-regulated. Stimulation of B cells with 100 ng/ml phorbol 12-myristate 13-acetate induced similar alteration, although only after 48-h treatment. The accumulation of Fc gamma RIIb1 and the reduction of both Fc gamma IIb2 and Fc gamma IIa mRNA in activated cells is accompanied by the enhanced expession of Fc gamma RII on the cell surface, representing most probably the Fc gamma RIIb1 isoform. Heat-aggregated IgG inhibited the anti-IgM-induced proliferation of resting but not that of activated B cells, suggesting that aggregation of Fc gamma RIIb2 constitutively expressed on resting B cells might be responsible for the prevention of inadequate activation of resting B cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
FC-GAMMA-RIIB
B CELLS
REGULATION
Megjelenés:European Journal of Immunology. - 25 : 1 (1995), p. 262-268. -
További szerzők:Rozsnyay Zoltán Koncz Gábor (1970-) (biológus, immunológus) Danilkovich, Alla Gergely János
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1