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001-es BibID:	BIBFORM065026
Első szerző:	Kovács Csilla (élelmiszerbiztonsági és minőségi mérnök)
Cím:	Mycelial growth rate and mycoparasitic potential of Trichoderma harzianum isolates against Fusarium oxysporum from grapevine / Csilla Kovács, Erzsébet Sándor, Ferenc Peles
Dátum:	2014
ISSN:	1217-8950 1588-2640
Megjegyzések:	Trichoderma harzianum is mycoparasite with antagonistic and mycotoxic metabolite production properties. Trichoderma species can be used as biopesticides against pathogenic fungi. Currently there is no effective fungicide against Fusarium oxysporum in grapevine trunk; therefore our aim was to find potential biopesticide against it.Eight mycoparasitic Tichoderma harzianum isolates (TR01-TR05; TR07; TR09; TR10) were tested in vitro to examine their mycelial growth rate. Those were isolated from grapevine trunk disease (GTD) asymptomatic grapevine trunks from the Tokaj wine region, Hungary. The average linear growth rate (ALG) of the isolates was tested at five different temperatures (18.5-37C). The mycoparasitic ability of the Trichoderma isolates against Fusarium oxysporum isolated from GTD symptomatic plants was also determined. 5 mm mycelial disks from the margin of seven days old colonies were cut, and put in 3 cm distance on PDA plates, than incubated at room temperature for 6 days. Finally growth inhibition value was calculated to express the mycoparasitic potential.Significant difference was found between the mycelial growth of the isolates. TR03 and TR08 isolates had significant lower growth rate, than the other Trichoderma isolates. The TR07, TR09 and TR10 isolates grew rapidly in all examined temperatures.The interaction between Trichoderma harzianum and Fusarium oxysporum f. sp. ciceris showed different inhibition ratio on the fourth and sixth day. The TR08 - F. oxysporum had the highest inhibition zone (4 day: 63.6%; 6. day: 82.6%), while TR10 - Fusarium oxysporum had the lowest one (4. day: 40.9%; 6. day: 47.3%). The TR09 isolate showed low (<50%) inhibition zone at the examined days, while TR07, the most intensively growing isolate also had high (> 50%) inhibition rate.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idézhető absztrakt Mycelial growth rate mycoparasitic potential Trichoderma harzianum
Megjelenés:	Acta Microbiologica et Immunologica Hungarica. - 62 : Suppl. (2014), p. 47-48. -
További szerzők:	Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Peles Ferenc (1979-) (mikrobiológia, élelmiszer-mikrobiológia, minőségügy)
Pályázati támogatás:	4.2.4.A/2-11-1-2012-0001 TÁMOP COST FA1303 Egyéb Sándor Erzsébet munkáját a Debreceni Egyetem belső kutatási pályázata támogatta Egyéb
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