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001-es BibID:	BIBFORM065055
Első szerző:	Kovács Csilla (élelmiszerbiztonsági és minőségi mérnök)
Cím:	Potential biopesticide against GTD pathogens isolated from asymptomatic grapevines / C. Kovács, F. Peles, E. Sándor
Dátum:	2015
Megjegyzések:	Grapevine trunk diseases (GTD), caused by different phytopathogenic fungi threaten the vineyards worldwide. Endophytic, mycoparasitic fungi, like Trichoderma species have huge potential against grapevine plant pathogens colonizing woody tissues. Testing new isolates with biocontrol potential and ability to grow under different environmental conditions may provide effective biopesticide to control GTD pathogens.Endophytic Trichoderma isolates were obtained from asymptomatic plants in the Tokaj Wine Region Hungary. Their mycelial growth rates were tested on different temperatures. The mycoparasitic potential of the trichodermas against two GTD pathogens (Diplodia seriata and Neofusicoccum parvum) was also tested.Eight Trichoderma harzianum were identified based on their ITS1,2 (White et al., 1990; Academic Press, San Diego, CA. 315-322), tef1 (Carbone and Kohn, (1999; Mycologia 91: 553-556) and Chit42-1a; 2a marker sequences (Kullnig-Gradinger et al., 2002; Mycological Research 106: 7. 757-767). The mycelial growth was determined from the two average colony diameters on PDA, while the mycoparasitic potential was detected according the method of Szekeres et al. (2006; Journal of Microbiological Methods, 619-622). Field test with mixed spore suspension of the three fastest-growing Trichoderma strains (107 conidia ml-1) on the pruning wounds of canes of GTD symptomatic grapevines were also tested.All the tested eight T. harzanum isolates were able to grow on the tested temperatures (5-37 ?C), but they showed different mycelial growth activity. 30 ?C were the optimal for all isolates. TR02-03 and TR05 had the highest growth rate at all the tested temperatures on PDA. TR01-05 and TR09 isolates showed the most intensive growth at the lowest temperature (5?C). TR08 and TR10 grew significantly slower at the lowest, while TR07, TR09 and TR10 at the highest tested temperature.The Biocontrol Index of all T. harzianum was 100%. Eleven GTD symptomatic plants were treated with Trichoderma spore suspension, containing TR04 and TR05 spores collected from seven day old colonies. The diseases-specific symptoms decreased in all but one of the treated trunks. Moreover the T. harzianum could be reisolated from their woody tissues.The tested T. harzianum are potential biopesticide, overgrowing GTD pathogens and decreasing symptoms on grape plants with the ability of growing well on broad temperature range.
ISBN:	978-3-9816508-7-7
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok előadáskivonat Potential biopesticide GTD pathogens asymptomatic grapevines
Megjelenés:	XVIII. International Plant Protection Congress Berlin, 24-28 August 2015. Program and Book of Abstracts / [ed.] Falko Feldmann, E. A. Short Heinrichs. - p. 444. -
További szerzők:	Peles Ferenc (1979-) (mikrobiológia, élelmiszer-mikrobiológia, minőségügy) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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