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001-es BibID:	BIBFORM066410
Első szerző:	Kristóf Endre (általános orvos)
Cím:	Role of ICAM3 and its interacting partners in the phagocytosis of apoptotic neutrophils by macrophages / E. K. Kristóf, G. Zahuczky, L. Fésüs
Dátum:	2012
ISSN:	1742-464X
Megjegyzések:	Apoptotic cells express eat-me signals which are recognized by several receptors mainly on professional phagocytes that can define their safe and effective clearance in order to maintain tissue homeostasis in the entire body. TLDA measurements predicted important role of some apopto-phagocytic genes in phagocytosis of apoptotic neutrophils by macrophages, because their expression level heavily elevated during the early stage of phagocytosis. After they were silenced by RNA interference, significant decrease in phagocytosis was observed only after silencing ICAM3. Our goal was to investigate the role and interacting partners of ICAM3 from both sides. Monocytes were isolated from buffy coats of healthy blood by CD14 specific magnetic separation. To examine phagocytic capacity of differentiated macrophages, apoptotic neutrophils were isolated from human blood by density-gradient centrifugation. Phagocytosis assay was performed using fluorescent labeled cells and the incorporated cell-rate was measured by flow cytometry, immediately after preincubation of macrophages or apoptotic cells with blocking antibodies. Localization of the investigated receptors was visualized by indirect immunostaining. Significant reduction of phagocytosis was noticed after blocking of ICAM3 from both sides. In macrophages but not in neutrophils silencing and blocking components of LFA-1, which can strongly bind ICAM3, resulted in a decreased phagocytosis of apoptotic cells. Engulfing caps formed in macrophages during phagocytosis are characterized by accumulation of ICAM3 and the subunits of LFA-1 which show colocalization on the surface of phagocytes showing that ICAM3 and LFA-1 act as recognition receptors in the phagocytosis portals of macrophages for engulfment of apoptotic neutrophils.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok poszter Molekuláris Medicina Doktori iskola
Megjelenés:	FEBS Journal 279 : Suppl. 1 (2012), p. 52. -
További szerzők:	Zahuczky Gábor (1975-) (molekuláris biológus, biokémikus, vegyész) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:	NK 105046 OTKA K 61868 OTKA TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Az apoptózis molekuláris mechanizmusa TÁMOP-4.2.2/B-10/1-2010-0024 TÁMOP Molekuláris Sejt- és Immunbiológia Doktori Iskola TRANSCOM IAPP251506 FP7 TRANSPATH ITN 289964 FP7
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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