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001-es BibID:	BIBFORM066424
Első szerző:	Kristóf Endre (általános orvos)
Cím:	Role of ICAM3 in the interaction between human macrophages and apoptotic neutrophil granulocytes / E. K. Kristóf, G. Zahuczky, L. Fésüs
Dátum:	2011
ISSN:	1742-464X
Megjegyzések:	The daily clearance of billions of physiologically dying cells in the human body is performed safely mainly by the mononuclear phagocyte system Bridging molecules and receptors ? which recognize the dying cells ? show high redundancy and are linked to several intracellular signaling pathways. Our previous ♭Taq-Man Low Density Array' measurements predicted important role of many genes in phagocytosis, because their expression level heavily elevated during the early stage of the phagocytosis process. The four genes (ADORA2A, FPRL1, ICAM3, THBS1)with the most enhanced expression in human macrophages wereknocked-down by RNA interference which was controlled byWestern-blot and surface immunostaining at translational level. Significant decrease in phagocytic capacity was observed only after silencing ICAM3. Our goal was to investigate the role and interacting partners of ICAM3 transmembrane protein from both sides. Human monocytes were isolated from ♭buffy coats' of healthy blood by magnetic separation using CD14 human microbeads. To examine the phagocytic capacity of 5 day differentiated macrophages, apoptotic neutrophil granulocytes were isolated from human blood by Histopaque density-gradient centrifugation. The phagocytosis assay was performed using fluorescent labeled cells and the incorporated cell-rate was measured by flow cytometry, immediately after pre-incubation of macrophages or apoptotic cells with blocking antibodies. Significant reduction of phagocytosis was noticed after blocking ICAM3 from both sides while that of DC-SIGN, which is a cited receptor for ICAM3, did not influence the engulfment of apoptotic cells. ICAM-3 participates in the recognition of apoptotic neutrophils by macrophages from both sides not only as an adhesion molecule but also as an initiator of signaling pathways mediated by intracellular thyrosin kinases. To examine its interacting partners in this process further investigations have to be performed in the future.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt Molekuláris Medicina
Megjelenés:	FEBS Journal 278 : Suppl. 1 (2011), p. 138. -
További szerzők:	Zahuczky Gábor (1975-) (molekuláris biológus, biokémikus, vegyész) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Az apoptózis molekuláris mechanizmusa NI 67877 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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