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001-es BibID:BIBFORM073406
Első szerző:Szilágyi Melinda (biológus)
Cím:Autolytic enzymes are responsible for increased melanization of carbon stressed Aspergillus nidulans cultures / Melinda Szilágyi, Fruzsina Anton, István Pócsi, Tamás Emri
Dátum:2018
ISSN:0233-111X
Megjegyzések:Melanization of carbon stressed Aspergillus nidulans cultures were studied. Melanin production showed strong positive correlation with the activity of the secreted chitinase and ?-1,3-glucanase. Deletion of either chiB encoding an autolyticendochitinase or engA encoding an autolytic ?-1,3-endoglucanase, or both, almost completely prevented melanization of carbon stressed cultures. In contrast, addition of Trichoderma lyticase to cultures induced melanin production. Synthetic melanin couldefficiently inhibit the purified ChiB chitinase activity. It could also efficiently decrease the intensity of hyphal fragmentation and pellet disorganization in Trichoderma lyticase treated cultures. Glyphosate, an inhibitor of L-3,4 dihydroxyphenylalanine type melanin synthesis, could prevent melanization of carbon-starved cultures and enhanced pellet disorganization, while pyroquilon, a 1,8-dihydroxynaphthalene-type melanin synthesis inhibitor, enhanced melanization, and prevented pellet disorganization. We concluded that cell wall stress induced by autolytic cell wall hydrolases was responsible for melanization of carbon-starved cultures. The produced melanin can shield the living cells but may not inhibit the degradation and reutilization of cell wall materials of dead hyphae. Controlling the activity of autolytic hydrolase productioncan be an efficient approach to prevent unwanted melanization in the fermentation industry, while applying melanin synthesis inhibitors can decrease the resistance of pathogenic fungi against the chitinases produced by the host organism.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of Basic Microbiology 58 : 5 (2018), p. 440-447. -
További szerzők:Anton Fruzsina Pócsi István (1961-) (vegyész) Emri Tamás (1969-) (biológus)
Pályázati támogatás:K112181
OTKA
Internet cím:DOI
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