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001-es BibID:	BIBFORM079006
Első szerző:	Mudambi, Shaila
Cím:	Photodynamic therapy does not induce cyclobutane pyrimidine dimers in the presence of melanin / Shaila Mudambi, Paula Pera, Deschana Washington, Remenyik Éva, Fidrus Eszter, Gal Shafirstein, Bellnier Dávid, Paragh György
Dátum:	2018
Megjegyzések:	Photodynamic therapy (PDT) is an office-based treatment for precancerous and early cancerous skin changes. PDT induces cell death through the production of reactive oxygen species (ROS). Cyclobutane pyrimidine dimers (CPDs) are the most important DNA changes responsible for ultraviolet (UV) carcinogenesis. Recently ROS induced by UVA were shown to generate CPDs via activating melanin. This raised the possibility that PDT induced ROS may also induce CPDs and mutagenesis in melanin containing cells. Previously the effect of PDT on CPDs in melanin containing cells has not been assessed. Our current work aimed to compare the generation of CPDs in melanin containing cells subjected to UVA treatment and porfimer sodium red light PDT. We used ELISA to detect CPDs. After UVA we found a dose dependent increase in CPDs in melanoma cells (B16-F10, MNT-1) with CPD levels peaking hours after discontinuation of UVA treatment. This indicated the generation of UVA induced dark-CPDs in the model. Nevertheless, PDT in biologically relevant doses was unable to induce CPDs. Our work provides evidence for the lack of CPD generation by PDT in melanin containing cells.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Skin Photodynamic therapy (PDT) Cyclobutane pyrimidine dimers (CPD) Reactive oxygen species (ROS) Melanin Ultraviolet A light (UVA) Melanocyte
Megjelenés:	Photodiagnosis and Photodynamic Therapy. - 22 (2018), p. 241-244. -
További szerzők:	Pera, Paula Washington, Deschana Remenyik Éva (1956-) (bőrgyógyász) Fidrus Eszter (1992-) (biotechnológus) Shafirstein, Gal Bellnier Dávid Paragh György Jr. (1978-) (bőrgyógyász)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00005 GINOP K120206 OTKA
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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