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001-es BibID:	BIBFORM084963
Első szerző:	Kövér Katalin, E. (vegyész)
Cím:	More line narrowing in TROSY by decoupling of long-range couplings: shift correlation and 1JNC· coupling constant measurements / Kövér Katalin E., Batta Gyula
Dátum:	2004
ISSN:	1090-7807
Megjegyzések:	Since the introduction of RDCs in high-resolution NMR studies of macromolecules, there is a growing interest in the development of accurate, and sensitive methods for determining coupling constants. Most methods for extracting these couplings are based on the measurement of the splitting between multiplet components in J-coupled spectra. However, these methods are often unreliable since undesired multiple-bond couplings can considerably broaden the multiplet components and consequently make accurate determination of their position difficult. To demonstrate one approach to this problem, G-BIRD(r) decoupled TROSY sequences are proposed for the measurement of 1JNH and 1JNC0 coupling constants. Resolved or unresolved splittings due to remote protons are removed by a G-BIRD(r) module employed during t1 and as a result, spectra with narrow, well-resolved peaks are obtained from which heteronuclear one-bond couplings can be accurately measured. Moreover, introduction of a spin-state-selective a/b-filter in the TROSY sequence allows the separation of the 1JNC0 doublet components into two subspectra which contain the same number of peaks as the regular TROSY spectrum. The 1JNC0 couplings are obtained from the displacement between the corresponding peaks in the subspectra.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk TROSY G-BIRD(r) Spin-state-selective a/b-filter Scalar couplings 1JNH 1JNC0 Protein
Megjelenés:	Journal Of Magnetic Resonance. - 170 : 2 (2004), p. 184-190. -
További szerzők:	Batta Gyula (1953-) (molekula-szerkezet kutató)
Pályázati támogatás:	T 042567 OTKA M 36776 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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