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001-es BibID:	BIBFORM085966
Első szerző:	Szántó Gábor Tibor (vegyész)
Cím:	The activation gate controls steady-state inactivation and recovery from inactivation in Shaker / Szanto Tibor G., Zakany Florina, Papp Ferenc, Varga Zoltan, Deutsch Carol J., Panyi Gyorgy
Dátum:	2020
ISSN:	0022-1295 1540-7748
Megjegyzések:	Despite major advances in the structure determination of ion channels, the sequence of molecular rearrangements at negative membrane potentials in voltage-gated potassium channels of the Shaker family remains unknown. Four major composite gating states are documented during the gating process: closed (C), open (O), open-inactivated (OI), and closed-inactivated (CI). Although many steps in the gating cycle have been clarified experimentally, the development of steady-state inactivation at negative membrane potentials and mandatory gating transitions for recovery from inactivation have not been elucidated. In this study, we exploit the biophysical properties of Shaker-IR mutants T449A/V474C and T449A/V476C to evaluate the status of the activation and inactivation gates during steady-state inactivation and upon locking the channel open with intracellular Cd 2+ . We conclude that at negative membrane potentials, the gating scheme of Shaker channels can be refined in two aspects. First, the most likely pathway for the development of steady-state inactivation is C?O?OI#CI. Second, the OI?CI transition is a prerequisite for recovery from inactivation. These findings are in accordance with the widely accepted view that tight coupling is present between the activation and C-type inactivation gates in Shaker and underscore the role of steady-state inactivation and recovery from inactivation as determinants of excitability.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Journal Of General Physiology. - 152 : 8 (2020), p. 1-12. -
További szerzők:	Zákány Florina (1989-) (általános orvos) Papp Ferenc (1979-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító) Deutsch, Carol Panyi György (1966-) (biofizikus)
Pályázati támogatás:	KTIA_NAP_13-2-2015-0009 MTA KTIA_NAP_132-2017-0013 MTA EFOP-3.6.2-16-2017-00006 EFOP GINOP-2.3.2-15-2016-00015 GINOP NKFIH K132906 OTKA NKFIH K119417 OTKA ÚNKP-19-3-III-DE-92 Egyéb GM 52302 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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