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001-es BibID:BIBFORM099567
035-os BibID:(WoS)000292280900044 (Scopus)79959926784
Első szerző:Szabó Zoltán
Cím:Rapid high resolution characterization of functionally important monoclonal antibody N-Glycans by Capillary Electrophoresis / Zoltan Szabo, András Guttman, Jonathan Bones, Barry L. Karger
Dátum:2011
ISSN:0003-2700
Megjegyzések:Characterization of the N-glycosylation present in the Fc region of therapeutic monoclonal antibodies requires rapid, high-resolution separation methods to guarantee product safety and efficacy during all stages of process development. Determination of fucosylated oligosaccharides is particularly important during clone selection, product characterization, and lot release as fucose has been shown to adversely affect the ability of mAbs to induce antibody dependent cellular cytotoxicity (ADCC). Here, we apply a general capillary electrophoresis optimization strategy to separate functionally relevant fucosylated and afucosylated glycans on mononclonal antibody products in the presence of several high mannose oligosaccharides. The N-glycans chosen represent those most commonly reported on CHO cell derived therapeutic antibodies. A rapid (<7 min) high-resolution separation of 12 commonly reported and functionally important IgG glycans was developed by systematically evaluating the effects of selectivity (boric acid) and efficiency (linear polyacrylamide) enhancing additives. The approach can be used to rapidly optimize capillary electrophoresis separation of other glycan mixtures. Following optimization, the method was applied to overnight sample processing for automated 96 well plate-based glycosylation analyses of two nonproprietary therapeutic monoclonal antibodies, demonstrating ruggedness and suitability for high-throughput process and product monitoring applications.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
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Megjelenés:Analytical Chemistry. - 83 : 13 (2011), p. 5329-5336. -
További szerzők:Guttman András (1954-) (vegyészmérnök) Bones, Jonathan Karger, Barry
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