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001-es BibID:BIBFORM102713
Első szerző:Shaw, Abhirup
Cím:Irisin stimulates the release of CXCL1 via upregulation of NFkB pathway from human neck derived differentiating adipocytes / Shaw Abhirup, B. Tóth Beáta, Győry Ferenc, Fésüs László, Kristóf Endre
Dátum:2022
ISSN:2211-5463
Megjegyzések:Obesity is at present a global epidemic. Brown and beige adipocytes function in utilizing excess fat to produce heat (thermogenesis), thereby counteracting obesity. Recent studies in rodents and humans indicated that these adipocytes also release cytokines, which may play a vital role in maintaining whole body energy homeostasis. Irisin is primarily released by myocytes during exercise and functions as a polypeptide regulator of beige adipocytes. We intended to characterize the effect of irisin on differentiating adipocytes derived from human subcutaneous neck (SC) and deep neck (DN) adipose tissue depots. Preadipocytes were isolated from SC and DN biopsies of the same donor, differentiated to adipocytes in the presence or absence of irisin. Global gene expression analysis was performed on nine independent donors. Irisin could not upregulate characteristic thermogenic genes, but upregulated genes related to several cytokines. Out of them,CXCL1 (the highest upregulated) was found to be released throughout the differentiation period, predominantly by SC and DN differentiated adipocytes. DN tissue biopsies showed a significant release of CXCL1 upon 24 hours irisin treatment. Geneexpression data indicated upregulation of the NFkB pathway upon irisin treatment, which was validated by an increase of p50 and decrease of IkBa protein level, respectively. Continuous blocking of the NFkB pathway by SN50 (cell permeable inhibitorof NFkB nuclear translocation) significantly reduced the release of CXCL1. The released CXCL1 exerted a positive effect on the adhesion capability of endothelial cells. Together, our findings demonstrate that irisin stimulates the release of a novel adipokine, CXCL1, via upregulation of NFkB pathway in human neck area derived differentiating adipocytes, which plays an important role in improving tissue vascularization (Previously published in:Shaw A et al. (2021) Front Cell Dev Biol 9:737872).
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:FEBS Open Bio. - 12 : S1 (2022), p. 208. -
További szerzők:Bartáné Tóth Beáta (1970-) (molekuláris biológus) Győry Ferenc (1964-) (sebész) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:FK131424
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GINOP-2.3.2-15-2016-00006
GINOP
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