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001-es BibID:	BIBFORM106821
035-os BibID:	(cikkazonosító)339828 (scopus)85128474989 (wos)000804191600005
Első szerző:	Shrivastava, Anuj
Cím:	N-Glycosylation of monoclonal antibody therapeutics : A comprehensive review on significance and characterization / Shrivastava Anuj, Joshi Srishti, Guttman Andras, Rathore Anurag S.
Dátum:	2022
ISSN:	0003-2670
Megjegyzések:	N-glycosylation of therapeutic antibodies starts as a co-translational step followed by a set of post-translational modifications and is considered as one of the critical quality attributes because of its impact on biological functions as well as therapy outcome. In addition to detailed product characterization of these glycans by the manufacturers, their comprehensive analysis is also a regulatory requirement. However, the structural complexity and heterogeneity of these N-linked carbohydrates make their characterization quite challenging. In this review, we give a comprehensive overview of N-glycosylation diversity and its functional importance for monoclonal antibody therapeutic products. A descriptive coverage is also provided for the various strategies and techniques employed for oligosaccharide characterization, that include analysis of intact-glycoproteins, their sub-units, glycopeptides as well as released glycans through chromatographic, electrophoretic and spectroscopic techniques. To assist the readers, relevant examples from the literature are cited and critically discussed for each of the strategies and techniques. To conclude, a discussion on unique challenges associated with the analysis of this important post-translational modification is presented.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Analytica Chimica Acta. - 1209 (2022), p. 1-18. -
További szerzők:	Joshi, Srishti Guttman András (1954-) (vegyészmérnök) Rathore, Anurag S.
Pályázati támogatás:	2018-2.1.17-TÉT-KR-201800010 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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