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001-es BibID:	BIBFORM113960
035-os BibID:	(WoS)001043559400001 (Scopus)85166981215
Első szerző:	Auer, Felicia
Cím:	Size separation of sodium dodecyl sulfate-proteins by capillary electrophoresis in dilute and ultra-dilute dextran solutions / Felicia Auer, Andras Guttman
Dátum:	2023
ISSN:	0173-0835 1522-2683
Megjegyzések:	SDS capillary gel electrophoresis is a widely used in the biopharma and the biomedical fields for rapid size separation of proteins. However, very limited information is available on the use of dilute and ultra-dilute sieving matrices for SDS?protein analysis. Here, background electrolytes (BGEs) containing 1%? 0% dextran were used in borate-based BGE to separate a protein sizing ladder (PSL) ?225 kDa and the intact and subunit forms of a therapeutic monoclonal antibody (mAb). The separation performance for the PSL and mAb components differed significantly with decreasing dextran concentration. Ferguson and reptation plots were used to elucidate the separation mechanism. Highly diluted dextran solutions resulted in linear Ferguson plots for both solute types (cf. Ogston theory) in spite of this model assumes a rigid pore structure, thus cannot describe the separation mechanism in ultra-dilute polymer solutions with no reticulations. The saddle differences between the resolution of the PSL and the intact/subunit mAb forms in ultra-dilute dextran-borate matrices suggested the importance of shape selectivity, manifested by the adequate separation of the SDS covered intact as well as light and heavy chain subunits of the therapeutic mAb even at zero dextran concentration.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk borate capillary gel electrophoresis dextran SDS-proteins ultra-dilute polymer
Megjelenés:	Electrophoresis. - 44 : 19-20 (2023), p. 1607-1614. -
További szerzők:	Guttman András (1954-) (vegyészmérnök)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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