Találati lista | Tudóstér

001-es BibID:	BIBFORM115946
035-os BibID:	(cikkazonosító)117397 (scopus)85175539890
Első szerző:	Rathore, Anurag S.
Cím:	Recent progress in high-throughput and automated characterization of N-glycans in monoclonal antibodies / Rathore Anurag S., Guttman Andras, Shrivastava Anuj, Joshi Srishti
Dátum:	2023
ISSN:	0165-9936
Megjegyzések:	N-glycosylation of monoclonal antibody therapeutics is a post-translation modification and a critical quality attribute because of its significant impact on the bioavailability as well as the efficacy of these products. The structural complexity and heterogeneity of the asparagine linked oligosaccharides on monoclonal antibodies present a challenge towards their characterization. In order to control and monitor these N-glycosylations during commercial manufacturing of biotherapeutics, there is a continuous need for rapid, sensitive, robust, and affordable characterisation strategies. In this review, our primary focus is on recent advancements in high- throughput and automated techniques for sample preparation, data acquisition and analysis of monoclonal antibody N-glycans since 2015. This includes characterization at the intact glycoprotein, middle up, glycopeptide, and released glycan levels with the use of high-throughput and automated techniques. Bioinformatic tools have also been conferred including various databases, data analysis softwares, and computational tools for glycan structure builders.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk N-Glycosylation IgG Biosimilars Critical quality attributes Automation High throughput Bioinformatics
Megjelenés:	Trac-Trends In Analytical Chemistry. - 169 (2023), p. 1-19. -
További szerzők:	Guttman András (1954-) (vegyészmérnök) Shrivastava, Anuj Joshi, Srishti
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: