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001-es BibID:	BIBFORM116412
035-os BibID:	(cikkazonosító)14891 (scopus)85174693451 (wos)001085010700001
Első szerző:	Alimohammadi, Shahrzad (Gyógyszerész)
Cím:	Factors Influencing Marker Expressions of Cultured Human Cord Blood-Derived Mast Cells / Alimohammadi Shahrzad, Masuda-Kuroki Kana, Szöllősi Attila Gábor, Di Nardo Anna
Dátum:	2023
ISSN:	1422-0067
Megjegyzések:	Mast cells (MCs) are tissue-resident immune cells of a hematopoietic origin that play vital roles in innate and adaptive immunity. Human MCs can be isolated and differentiated from various tissue sources, including cord blood, when supplemented with cytokines such as stem cell factor, interleukin 3, and interleukin 6. Our current research study has shown significant differences in the marker expressions of human cord blood-derived mast cells (hCBMCs) based on donor dependency and the type of medium used for culturing and differentiation. These findings are particularly relevant given the challenges of obtaining specialty media influencing MC phenotypic marker expressions. We found that hCBMCs cultured in StemSpanTM-XF medium had a moderate expression of mast/stem cell growth factor receptor Kit (c-KIT) (mRNA and protein), low expressions of Fc?RI (mRNA) and TLR2 (mRNA and protein) but had high levels of MRGPRX2 (mRNA and protein) expressions. In contrast, hCBMCs cultured in Stem Line II medium expressed Fc?RI and TLR2 (mRNA and protein) with higher c-KIT but had lower MRGPRX2 expressions compared to the hCBMCs cultured in the StemSpanTM-XF medium. These results suggest that it is crucial to consider both donor dependency and the medium when investigating MC functions and that further research is needed to fully understand the impact of these factors on the hCBMC marker expressions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk human stem cells CD34+ cord blood-derived mast cells immune response c-KIT Fc?RI MRGPRX2 TLR2 tryptase chymase
Megjelenés:	International Journal Of Molecular Sciences. - 24 : 19 (2023), p. 1-11. -
További szerzők:	Masuda-Kuroki, Kana Szöllősi Attila Gábor (1982-) (élettanász) Di Nardo, Anna
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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