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001-es BibID:	BIBFORM118852
Első szerző:	Szarvas József
Cím:	Comparative studies on the cultivation and phylogenetics of King Oyster Mushroom (Pleurotus eryngii (DC.: Fr.) Quél.) strains / Szarvas József; Pál Károly; Geösel András; Győrfi Júlia
Dátum:	2011
Megjegyzések:	The king oyster mushroom (Pleurotus eryngii) is still not a well known species amongst producers, despite its excellent taste and relatively easy cultivation technologies. Nevertheless, European growers show growing interest towards it. We collected king oyster mushroom strains from various habitats to get a better view about their vegetative growth, molecular based taxonomic relationship and cultivation parame- ters. In the in vitro experiments we investigated the growth of vegetative mycelia at various temperatures and pH. All strains were cultivated on lignocellulose substrate for 60 days under adequate climatic conditions and yield quantity, flushes, number and average weight of fruiting bodies were determined. Biological Efficiency (BE%) and Productivity (P%) were calculated for each strains. We investigated taxonomic relationships among P. eryngii isolates by means of RAPD-PCR method. Twenty-five random primers were tested and six of them supplied us with sufficient data for generation of a neighbour-joining tree. Results of the cultivation experiments showed that environmental conditions resulted in a very different growth rate of the various P. eryn- gii strains on different temperature and pH. Calculated for 100 kg sub- strate the highest yield was produced by the Ple-4V strain (41.5 kg), whereas the lowest yield was found at the PEL isolate (9 kg). The av- erage yield of the investigated strains was 27.53 kg. Average weight per fruiting body was 19.95 g. We found the highest BE% at the Ple-4V and the lowest BE% at the PEL strains, 156.18% and 28.52%, respectively. These results showed that cultivation properties of the isolates are very different. The neighbour-joining tree revealed the taxonomic relations amongst the Hungarian, Malaysian, Italian and Dutch isolates. No cor- relation was found between the taxonomical position and growth rate of the investigated isolates. The average yield showed that the P. eryn- gii might be a suitable alternative of the popular tree oyster mushroom (P. ostreatus). Our results can be useful for subsequent cross breeding experiments and the dataset might support further investigations.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk vegetative mycelium RAPD-PCR neighbour-joining tree biological efficiency
Megjelenés:	Acta Universitatis Sapientiae: Agriculture and Environment. - 3 (2011), p. 18-34. -
További szerzők:	Pál Károly (1978-) (mikrobiológus) Geösel András Győrfi Júlia
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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