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001-es BibID:	BIBFORM119720
Első szerző:	Elshafia, Ali Hamid Mohammed agrármérnök
Cím:	Identification of Antibiotic-Resistant Bacteria in Commercial Fish Farms in Hungary / Elshafia Ali Hamid Mohammed; Renáta Bőkényné Tóth; Gábor Kardos; Pál Károly
Dátum:	2024
Megjegyzések:	The common carp (Cyprinus carpio) is a popular species in fish farms in Hungary (Europe). Since Hungary is a member state of the European Union, the use of antibiotics for growth promotion of animals has been banned since 2011. An even stricter legislation came into force in 2022 to inhibit the spread of antibiotic resistant bacteria in animals and humans, in accordance with the Farm to Fork Strategy. Nevertheless, new strains of AMR conferring bacteria may appear in fish farm lakes as a result of antibiotic treatment of bacterial infections, or due to wild birds as reservoirs. Antibiotic resistance genes (especially in form of mobile genetic elements, e.g. plasmids) may pose a serious threat to the fish production, health of employees, and environment. The current study aimed to survey the occurrence of antibiotic resistant bacteria and confirm their identity in water samples of commercial fish farms in Hungary. Twenty water samples were collected from two fish farms and subjected to bacterial isolation using a membrane filtration technique (pore size: 0.45-micrometer). Then the membranes were placed on different types of selected media (VRE, Eosin Methylene Blue and Endo's Medium). Bacterial colonies were subjected to matrix-assisted laser desorption ionization?timeof- flight mass spectrometry (MALDI-TOF MS) technique for species level identification. The following species were detected: Acinetobacter baumannii, A. calcoacetius, A. junii, A. pittii, A. tandoii, Bacillus pumilus, Comamonas aquatica, Enterococcus casseliflavus, E. gallinarum, Escherichia coli, Lactobacillus plantarum, Ochrobactrum intermedium, Pediococcus pentosaceus, Pseudomonas aeruginosa, P. alcaligenes, P. mendocina, P. otidis, P. putida and Stenotrophomonas maltophilia. In the next step, we will use antibiotic tests to investigate, whether these isolates have some unusual extrinsic antibiotic resistance. In addition, we will perform PCR experiments to detect the presence of virulence factors and toxin producing genes.
Tárgyszavak:	Orvostudományok Egészségtudományok előadáskivonat könyvrészlet AMR antibiotic resistant bacteria Common carp MALDI-TOF MS
Megjelenés:	15th Egerton University Biennial International Conference : Programme and book of abstracts / ed. M.K. Charimbu. - p. 42
További szerzők:	Bőkényné Tóth Renáta Kardos Gábor (1974-) (absztraktok) Pál Károly (1978-) (mikrobiológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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