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001-es BibID:	BIBFORM119831
035-os BibID:	(cikkazonosító)9503 (Scopus)85136696368 (WoS)000846624400001
Első szerző:	Bánfalvi Gáspár (sejtbiológus, gyógyszerész)
Cím:	Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes / Gaspar Banfalvi
Dátum:	2022
ISSN:	1422-0067
Megjegyzések:	Eukaryotic chromosomes have not been visualized during the interphase. The fact that chromosomes cannot be seen during the interphase of the cell cycle does not mean that there are no means to make them visible. This work provides visual evidence that reversible permeabilization of the cell membrane followed by the regeneration of cell membranes allows getting a glimpse behind the nuclear curtain. Reversibly permeable eukaryotic cells have been used to synthesize nascent DNA, analyze the 5 '-end of RNA primers, view individual replicons and visualize interphase chromosomes. Dextran T-150 in a slightly hypotonic buffer prevented cells from disruption. Upon reversal of permeabilization, the nucleus could be opened at any time during the interphase. A broad spectrum of a flexible chromatin folding pattern was revealed through a series of transient geometric forms of chromosomes. Linear attachment of chromosomes was visualized in several mammalian and lower eukaryotic cells. The linear connection of chromosomes is maintained throughout the cell cycle showing that rather than individual chromosomes, a linear array of chromosomes is the functional giant macromolecule. This study proves that not only the prokaryotic genome but also linearly attached eukaryotic chromosomes form a giant macromolecular unit.
Tárgyszavak:	Természettudományok Biológiai tudományok magyar nyelvű folyóiratközlemény külföldi lapban folyóiratcikk chromosome isolation DNA replication forms of interphase chromosomes nascent DNA reversible permeabilization
Megjelenés:	International Journal Of Molecular Sciences. - 23 : 16 (2022), p. 1-19. -
Pályázati támogatás:	42762 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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