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001-es BibID:	BIBFORM043761
Első szerző:	Fári Miklós (kertészmérnök)
Cím:	Agrobacterium-mediated genetic-transformation and plant-regeneration via organogenesis and somatic embryogenesis from cotyledon leaves in eggplant (solanum-melongena L CV kecskemeti-lila / Fari M., Nagy I., Csanyi ., Mityko J., Andrasfalvy A.
Dátum:	1995
ISSN:	0721-7714
Megjegyzések:	Novel and efficient protocols for plant regeneration genetic transformation longitudinally-halved cotyledons of in vitro seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation with Agrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgenic plantlets were regenerated on selective media containing 100 mg/l kanamycin. Transformants were recovered from embryogenic calli induced by 4 mg/l alpha-naphthaleneacetic acid (NAA), and from organogenic calli induced by the addition of 2 mg/l zeatin plus 0.01 mg/l NAA. Nineteen independent transgenic lines were grown to maturity. The structural integrity, expression and sexual transmission of the introduced genes for neomycin phosphotransferase and beta-glucuronidase (gus) were investigated.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Plant cell reports. - 15 : 1-2 (1995), p. 82-86. -
További szerzők:	Nagy István (agrár) Csányi M. Mitykó J. Andrásfalvy A.
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001-es BibID:	BIBFORM043760
Első szerző:	Fontes, M. A.
Cím:	Hyperhydricity in pepper plants regenerated in vitro: involvement of BiP (Binding Protein) and ultrastructural aspects / M. A. Fontes, W. C. Otoni, S. M. B. Carolino, S. H. Brommonschenkel, E. P. B. Fontes, M. Fari, R. P. Louro
Dátum:	1999
ISSN:	0721-7714
Megjegyzések:	Hyperhydricity in regenerated pepper plants was monitored by the induction of the ER-luminal resident protein, as observed by immunoblotting. Immunoblotting of total protein using an anti-soybean BiP serum indicated that the induction and accumulation of an 80-kDa protein was related to BiP (Binding protein), a 78-kDa ER-resident molecular chaperone. The anti-BiP serum cross-reacted with an 80-kDa protein which was significantly induced by hyperhydricity. Based on similar molecular weight and immunological reactivity we concluded that the 80-kDa protein induced in hyperhydric plants is a BiP homologue. The ultrastructural organisation of leaves in non-hyperhydric and hyperhydric pepper (Capsicum annuum L.) plants was investigated with the aim of identifying the subcellular changes associated with this phenomenon. In non-hyperhydric leaves the chloroplasts of the palisade cells had normally developed thylakoids and grana and a low accumulation or absence of starch grains and plastoglobules. In the hyperhydric plants, however, the chloroplasts exhibited thylakoid disorganisation, low grana number, an accumulation of large starch grains and a low accumulation or absence of plastoglobules. Although the structure of mitochondria and peroxisomes did not change in hyperhydric plants, the number of peroxisomes did increase.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Plant cell reports. - 19 : 1 (1999), p. 81-87. -
További szerzők:	Otoni, Wagner Campos Carolino, S. M. B. Brommonschenkel, S. H. Fontes, E. P. B. Fári Miklós (1953-) (kertészmérnök) Louro, R. P.
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001-es BibID:	BIBFORM043762
Első szerző:	Szász A. (agrár)
Cím:	Sceening for in-vitro shoot-forming capacity of seedling explants in bell pepper (Capsicum-Annuum-L) genotypes and efficient plant-regeneration using thidiazuron / Szasz A., Nervo G., Fari M.
Dátum:	1995
ISSN:	0721-7714
Megjegyzések:	In vitro shoot regeneration ability of 17 (7 Italian and 10 Hungarian) bell pepper genotypes was investigated using excised cotyledons and rooted hypocotyls as explants. Most of the Italian genotypes and two of the Hungarian genotypes responded well, producing shoots from rooted hypocotyls. Only two genotypes (one Italian and one Hungarian) gave a weak response using cotyledons. For direct shoot induction in these explants, in addition to the methods cited in the relevant papers, a new method was applied using thidiazuron as a cytokinin. Shoots were successfully regenerated from cotyledons of two Italian and two Hungarian genotypes using thidiazuron which were considered to be non responsive to the usual methods.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Plant cell report. - 14 : 10 (1995), p. 666-669. -
További szerzők:	Nervo G. (agrár) Fári Miklós (1953-) (kertészmérnök)
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