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001-es BibID:BIBFORM020261
Első szerző:Kaszanyitzky J. Éva
Cím:Staphylococci isolated from animals and food with phenotypically reduced susceptibility to beta-lactamase-resistant beta-lactam antibiotics / Kaszanyitzky É. J., Egyed Zs., Jánosi Sz., Keserű J., Gál Zs., Szabó I., Veres Z., Somogyi P.
Dátum:2004
ISSN:0236-6290
Megjegyzések:The antibiotic resistance pattern of 1921 Staphylococcus strains isolatedfrom animals and food within the last two years were examined using diffusiontests. Among them there were only 35 strains of S. aureus having an inhibitionzone diameter of 15 mm or less, and 4 strains of coagulase-negative staphylococci(CNS) having a zone diameter of 18 mm or less to 1-?g oxacillin disk. These 39strains were examined also by E-test to oxacillin and for the detection of the mecAgene by PCR in order to determine whether they might be real methicillinresistantstaphylococci. Among the 39 strains there were only two that were susceptibleto penicillin by disk diffusion method; however, further examination bythe penicillinase test showed that they produced beta-lactamase. While 19 (15 S.aureus, 4 CNS) strains were resistant and 7 strains were intermediate to oxacillinin disk diffusion test, the E-test gave 8 resistant and 5 intermediate results. Six outof the 8 oxacillin-resistant strains examined by disk diffusion and E-test harbouredthe mecA gene. Thus only 6 out of the examined 1921 strains proved to bemecA positive. These methicillin-resistant, mecA-positive strains (5 of the S.aureus strains and 1 of the S. epidermidis) originated from two dairy herds. Theresults prove that methicillin-resistant S. aureus (MRSA) strains in animals arereally rare in Hungary. Eighteen strains were chosen and screened for minimal inhibitoryconcentration (MIC) of oxacillin with or without clavulanic acid or sulbactam,and three of them produced methicillinase enzyme.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény hazai lapban
methicillin-resistant Staphylococcus aureus (MRSA)
methicillin- resistant coagulase negative staphylococci (CNS)
borderline resistance
Megjelenés:Acta Veterinaria Hungarica. - 52 : 1 (2004), p. 7-17. -
További szerzők:Egyed Zsuzsanna Jánosi Szilárd Keserű Judit (1976-) (molekuláris genetikus) Gál Zsuzsanna (1959-) (vegyész) Szabó István (1950-) (sejtbiológus, molekuláris genetikus) Veres Zoltán Somogyi Péter
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