CCL

Összesen 1 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM074763
Első szerző:Soltész Beáta (molekuláris biológus)
Cím:Determination of miR-193b rs30236 single nucleotide polymorphism in ovarian cancer patients / Soltész B., Lukács J., Penyige A., Póka R., Nagy B.
Dátum:2019
ISSN:0392-2936
Megjegyzések:Background:Single nucleotide polymorphism (SNP) is the most common form of genetic variability. The miR-193b was connectedto several types of tumors and also with occurrence of resistance to chemotherapy. This investigation aimed to determine miR-193brs30236polymorphism in ovarian cancer patients and controls. The authors performed bioinformatics analysis to discoverthe target genesof miR-193b. Materials and Methods: Eighty-six ovarian cancer patients and 102 matched controls were included in the study. Bloodsamples were drown into EDTA tubes and DNA was isolated by silica adsorption method. Mir-193b rs30236C/T polymorphism wasdetermined bymelting curve analysis. Chi-square test was used for statistical evaluation of the data.MiRTargetLinkwas used for de-termination of the has-miR-193b-3p microRNA target genes. Results: Based on melting curve analysis of miR-193b rs30236, the au-thors found 30.24% T allele frequency in the patients' group, and 35.78% in the control group (p = 0.2549). They detected CC genotypein 39.22%, CT genotype in 50.00%, and TT genotype in 10.78% of controls, while they occurred in 45.35%, 48.84%, and 5.81% of thepatients (p = 0.4096), respectively.The most important target genes of miR-193b are cyclin D1(CCND1), estrogen receptor(ESR1),plasminogen activator(PLAU), prolin rich acidic protein(PRAP1), and myeloid leukemia sequence(MCL1). Conclusions: The miR-193b has effect on the expression of several genes. The rs30236 polymorphism hasnot been studied in ovarian cancer patients, yet. Theauthors did not find significant difference in allele and genotype difference in patients and controls. To discover the exact role and suit-ability of this SNP for use as biomarker in the ovarian cancer prior to chemotherapy, more detailed studies are needed.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
miRNA
miR-193b
gene expression
ovarian cancer
melting curve analysis
Megjelenés:European Journal Of Gynaecological Oncology. - 40 : 4 (2019), p. 547-550. -
További szerzők:Lukács János (1975-) (szülész-nőgyógyász, genetikus) Penyige András (1954-) (molekuláris genetikus) Póka Róbert (1960-) (szülész-nőgyógyász, klinikai onkológus) Nagy Bálint (1956-) (molekuláris genetikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1