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1.

001-es BibID:BIBFORM005744
Első szerző:Kiss Zsuzsanna (genetikus)
Cím:Streptomyces griseus 45H, a producer of the extracellular autoregulator protein factor C, is a member of the species Streptomyces albidoflavus / Kiss Zsuzsanna, Ward Alan C., Birkó Zsuzsanna, Chater Keith F., Biró Sándor
Dátum:2008
Megjegyzések:Streptomyces griseus strain 45H, isolated in 1960 during a mutagenesis programme on the industrial streptomycin producer S. griseus 52-1, encodes an extracellular, pleiotropic autoregulatory signalling protein, factor C, which stimulates sporulation of S. griseus 52-1 in submerged culture. The facC gene, which codes for factor C, is present in very few streptomycetes and is not present in S. griseus 52-1. Based on 16S rRNA gene sequencing and other molecular data, S. griseus 45H, the factor C producer, is here shown to be related to the original laboratory strain of Streptomyces flavofungini, which was being studied in the same laboratory in 1960, and to Streptomyces albidoflavus. Southern blotting revealed that three out of four independently isolated strains of S. albidoflavus possess facC. Both the original strain of S. flavofungini and S. griseus 45H are therefore identified as members of the species Streptomyces albidoflavus, and we propose that S. griseus 45H should be renamed Streptomyces albidoflavus 45H.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
C-factor
Strepromyces
Megjelenés:International Journal of Systematic and Evolutionary Microbiology. - 58 : (Pt4) (2008), p. 1029-1031. -
További szerzők:Ward, Alan C. Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus) Chater, Keith F. Biró Sándor (1949-) (molekuláris genetikus)
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2.

001-es BibID:BIBFORM025198
Első szerző:Nagy Zoltán (biológus)
Cím:Carbon source regulation of β-galactosidase biosynthesis in Penicillium chrysogenum / Zoltán Nagy, Zsolt Keresztessy, Attila Szentirmai, Sándor Biró
Dátum:2001
Megjegyzések:Growth and beta-galactosidase activity of the penicillin producer industrial Penicillium chrysogenum NCAIM 00237 strain were examined using different carbon sources. Good growth was observed using glucose, sucrose, glycerol and galactose, while growth on lactose was substantially slower. beta-Galactosidase activity was high on lactose and very low on all the other carbon sources tested. In glucose grown cultures after exhaustion of glucose as repressing carbon source a derepressed low level of the enzyme was observed. cAMP concentration in lactose grown cultures was relatively high, in glucose grown cultures was low. Caffeine substantially decreased glucose consumption and growth but did not increase beta-galactosidase activity and did not prevent glucose repression which rules out the involvement of cAMP in the regulation of beta-galactosidase biosynthesis in Penicillium chrysogenum.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of Basic Microbiology. - 41 : 6 (2001), p. 351-362. -
További szerzők:Keresztessy Zsolt Szentirmai Attila (1930-) (mikrobiológus) Biró Sándor (1949-) (molekuláris genetikus)
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3.

001-es BibID:BIBFORM046865
Első szerző:Ochi, Kozo
Cím:The possible role of ADP-ribosylation in sporulation and streptomycin production by Streptomyces griseus / Ochi, K., Penyige, A., Barabás, Gy.
Dátum:1992
ISSN:0022-1287
Megjegyzések:Mutants resistant to 3-aminobenzamide, a known inhibitor of ADP-ribosyltransferase, were obtained from Streptomyces griseus IFO 13189, a streptomycin-producing strain. One (strain no. 4), which had significantly reduced ADP-ribosyltransferase activity, was analysed in detail. Mutant 4 displayed a conditional phenotype with respect to cultivation temperature. At 30 degrees C, it exhibited severely reduced ability to produce aerial mycelium (on solid medium) and submerged spores and streptomycin (in liquid culture), but this ability was fully restored at 25 degrees C. The mutant produced A-factor normally, regardless of cultivation temperature, and exhibited normal ability to accumulate ppGpp intracellularly. SDS-PAGE analyses of cellular proteins labelled by [32P]NAD revealed that an ADP-ribosylated protein with a molecular size of 44 kDa, which appeared in sporulating cultures of the parent strain, was missing from the mutant grown at the non-permissive temperature (30 degrees C). Genetic analysis showed that the aba mutation conferring resistance to 3-aminobenzamide was tightly linked to the altered phenotype. Failure to ADP-ribosylate certain cellular protein(s), presumably due to the aba mutation, may be responsible for impaired differentiation in this mutant.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of General Microbiology. - 138 : (Pt8) (1992), p. 1745-1750. -
További szerzők:Penyige András (1954-) (molekuláris genetikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus)
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4.

001-es BibID:BIBFORM039004
035-os BibID:PMID:19851727
Első szerző:Penyige András (molekuláris genetikus)
Cím:Analysis and identification of ADP-ribosylated proteins of Streptomyces coelicolor M145 / András Penyige, Judit Keseru, Ferenc Fazakas, Iván Schmelczer, Krisztina Szirák, György Barabás, Sándor Biró
Dátum:2009
ISSN:1225-8873
Megjegyzések:Mono-ADP-ribosylation is the enzymatic transfer of ADP-ribose from NAD(+) to acceptor proteins catalyzed by ADP-ribosyltransferases. Using m-aminophenylboronate affinity chromatography, 2D-gel electrophoresis, in-gel digestion and MALDI-TOF analysis we have identified eight in vitro ADP-ribosylated proteins in Streptomyces coelicolor, which can be classified into three categories: (i) secreted proteins; (ii) metabolic enzymes using NAD(+)/NADH or NADP(+)/NADPH as coenzymes; and (iii) other proteins. The secreted proteins could be classified into two functional categories: SCO2008 and SC05477 encode members of the family of periplasmic extracellular solute-binding proteins, and SCO6108 and SC01968 are secreted hydrolases. Dehydrogenases are encoded by SC04824 and SC04771. The other targets are GlnA (glutamine synthetase I., SC02198) and SpaA (starvation-sensing protein encoded by SC07629). SCO2008 protein and GlnA had been identified as ADP-ribosylated proteins in previous studies. With these results we provided experimental support for a previous suggestion that ADP-ribosylation may regulate membrane transport and localization of periplasmic proteins. Since ADP-ribosylation results in inactivation of the target protein, ADP-ribosylation of dehydrogenases might modulate crucial primary metabolic pathways in Streptomyces. Several of the proteins identified here could provide a strong connection between protein ADP-ribosylation and the regulation of morphological differentiation in S. coelicolor.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
protein ADP-ribosylation
Streptomyces coelicolor
MALDI-TOF
2-D PAGE
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal Of Microbiology. - 47 : 5 (2009), p. 549-556. -
További szerzők:Fazakas Ferenc (1969-) (molekuláris biológus) Schmelczer Iván Keserű Judit (1976-) (molekuláris genetikus) Szirák Krisztina (1973-) (molekuláris genetikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Biró Sándor (1949-) (molekuláris genetikus)
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5.

001-es BibID:BIBFORM053143
Első szerző:Springer, Jan
Cím:Multicenter comparison of serum and whole-blood specimens for detection of Aspergillus DNA in high-risk hematological patients / Jan Springer, C. O. Morton, Michael Perry, Werner J. Heinz, Melinda Paholcsek, Mona Alzheimer, T. R. Rogers, Rosemary A. Barnes, Hermann Einsele, Juergen Loeffler, P. Lewis White
Dátum:2013
ISSN:0095-1137
Megjegyzések:Samples from patients at high risk for invasive aspergillosis (IA) were prospectively collected and analyzed for the presence of molecular markers of fungal infection. Serum specimens were screened for galactomannan and Aspergillus DNA, and whole-blood specimens were screened only for Aspergillus DNA. Fungal infections were categorized according to the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group, National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) criteria. Forty-seven cases (proven and probable IA) and 31 controls (no evidence of IA) were selected retrospectively for this case-control study, comprising 803 samples, in order to determine the performance of whole-blood PCR, serum PCR, and serum galactomannan testing. Although no single assay was able to detect every case of IA, a combination of different assays provided the best performance. There was no significant difference between the use of whole-blood and serum specimens for PCR-based diagnosis of IA, but there was a trend for whole blood to be more sensitive (85% versus 79%) and to yield an earlier positive result (36 days versus 15 days) than for serum. However, DNA extraction from serum specimens is easier and faster than that from whole-blood specimens, and it allows the same specimen to be used for both galactomannan and PCR assays. In conclusion, the appropriate sample type for DNA extraction should be determined by the local requirements and the technical platforms available at each individual center. A combination of biomarker tests offered the best diagnostic utility for detecting IA.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
DIAGNOSING INVASIVE ASPERGILLOSIS
REAL-TIME PCR
FUNGAL-INFECTIONS
FUMIGATUS DNA
Megjelenés:Journal of Clinical Microbiology. - 51 : 5 (2013), p. 1445-1450. -
További szerzők:Morton, C. Oliver Perry, Michael Heinz, Werner J. Paholcsek Melinda (1984-) (molekuláris biológus, genetikus) Alzheimer, Mona Rogers, T. R. Barnes, Rosemary A. Einsele, Hermann Loeffler, Juergen White, P. Lewis
Pályázati támogatás:German Federal Ministry of Research and Education (BMBF)
Egyéb
Trans-European Cooperation ERA-NET PathoGenoMics
Egyéb
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6.

001-es BibID:BIBFORM077294
Első szerző:Szilágyi Melinda (biológus)
Cím:Mutation in afsR Leads to A-Factor Deficiency in Streptomyces griseus B2682 / Melinda Szilágyi, Éva Márton, Dávid Lukács, Zsuzsanna Birkó, Zoltán Kele, Sándor Biró
Dátum:2018
ISSN:1464-1801
Megjegyzések:A-factor, a γ-butyrolactone autoregulator, in Streptomyces griseus is involved in the regulation of differentiation and antibiotic production. Here we studied the S. griseus B2682-AFN (A-factor negative) bald mutant that harbors a nonsense mutation in the afsR gene encoding a pleiotropic regulator. Our aim was to prove that this mutation is the cause of the A-factor deficiency in AFN. We also studied whether AfsR regulates A-factor production by AfsA, which is supposed to be the only specific key enzyme in A-factor biosynthesis. METHODS: Wild afsR was cloned to the pHJL401 shuttle vector and was transformed to the S. griseus AFN and B2682 strains. During phenotypic characterization, sporulation, antibiotic, protease, A-factor, and AfsA protein production were studied. RESULTS: Transformation of AFN by a wild afsR restored its phenotype including sporulation, antibiotic, extracellular protease, and A-factor production. Introduction of afsR to the B2682 wild-type strain resulted in antibiotic and extracellular protease overproduction that was accompanied with an elevated A-factor level. AfsA was detected both in AFN and B2682. CONCLUSIONS: AfsR has an effect on the regulation of A-factor production in S. griseus. The presence of AfsA is not sufficient for normal A-factor production. AfsR regulates A-factor biosynthesis independently of AfsA.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Molecular Microbiology And Biotechnology. - 28 : 5 (2018), p. 216-224. -
További szerzők:Márton Éva (1992-) (biológus) Lukács Dávid Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus) Kele Zoltán Biró Sándor (1949-) (molekuláris genetikus)
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7.

001-es BibID:BIBFORM039003
035-os BibID:PMID:22752904
Első szerző:Szirák Krisztina (molekuláris genetikus)
Cím:Disruption of SCO5461 gene coding for a mono-ADP-ribosyltransferase enzyme produces a conditional pleiotropic phenotype affecting morphological differentiation and antibiotic production in Streptomyces coelicolor / Krisztina Szirák, Judit Keserű, Sándor Biró, Iván Schmelczer, György Barabás, András Penyige
Dátum:2012
ISSN:1225-8873
Megjegyzések:The SCO5461 gene of Streptomyces coelicolor A3(2) codes for an ADP-ribosyltransferase enzyme that is predicted to be a transmembrane protein with an extracellular catalytic domain. PCR-targeted disruption of the gene resulted in a mutant that differentiated normally on complex SFM medium; however, morphological differentiation in minimal medium was significantly delayed and this phenotype was even more pronounced on osmotically enhanced minimal medium. The mutant did not sporulate when it was grown on R5 medium, however the normal morphological differentiation was restored when the strain was cultivated beside the wild-type S. coelicolor M145 strain. Comparison of the pattern of ADP-ribosylated proteins showed a difference between the mutant and the wild type, fewer modified proteins were present in the cellular crude extract of the mutant strain. These results support our previous suggestions that protein ADP-ribosylation is involved in the regulation of differentiation and antibiotic production and secretion in Streptomyces.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Streptomyces
egyetemen (Magyarországon) készült közlemény
mono-ADP-ribosyltransferase
protein ADP-ribosylation
morphological differentiation
actinorhodin production and secretion
Megjelenés:Journal of Microbiology. - 50 : 3 (2012), p. 409-418. -
További szerzők:Schmelczer Iván Keserű Judit (1976-) (molekuláris genetikus) Biró Sándor (1949-) (molekuláris genetikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Penyige András (1954-) (molekuláris genetikus)
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8.

001-es BibID:BIBFORM025168
Első szerző:Vitális Sándor
Cím:Differentiation and its regulation in submerged culture of Streptomyces griseus / Sándor Vitális, Sándor Biró, György Vargha, István Békési, Gábor Szabó
Dátum:1981
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Zentralblatt für Bakteriologie -International journal of medical microbiology, virology, prasitology and infectious diseases. - Suppl. 11 (1981), p. 153-156. -
További szerzők:Biró Sándor (1949-) (molekuláris genetikus) Vargha György (1951-) (orvos) Szabó Gábor (1927-1996) (biológus, genetikus) Békési István
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