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001-es BibID:BIBFORM058666
Első szerző:Bacskai Ildikó (immunológus)
Cím:Mesenchymal stromal cell-like cells set the balance of stimulatory and inhibitory signals in monocyte-derived dendritic cells / Ildikó Bacskai, Anett Mázló, Katalin Kis-Tóth, Attila Szabó, György Panyi, Balázs Sarkadi, Ágota Apáti, Éva Rajnavölgyi
Dátum:2015
ISSN:1547-3287
Megjegyzések:The major reservoir of human multipotent mesenchymal stem/stromal cells (MSC) is the bone marrow (BM) with the capability to control hematopoietic stem cell (HSC) development. The regenerative potential of MSC is associated with enhanced endogenous repair and healing mechanisms that modulate inflammatory responses. Our previous results revealed that MSC-like (MSCl) cells derived from pluripotent human embryonic stem cells resemble BM-derived MSC in morphology, phenotype and differentiating potential. Here we investigated the effects of MSCl cells on the phenotype and functions of dendritic cells (DC). To assess how anti-viral immune responses could be regulated by intracellular pattern recognition receptors (PRR) of DC in the presence of MSCl cells we activated DC with the specific ligands of retinoic acid-inducible gene I (RIG-I) helicases and found that activated DC co-cultured with MSCl cells exhibited reduced expression of CD1a and CD83 cell surface molecules serving as phenotypic indicators of DC differentiation and activation, respectively. However, RIG-I-mediated stimulation of DC via specific ligands in the presence of MSCl cells resulted in significantly higher expression of the co-stimulatory molecules CD80 and CD86 than in the presence of BM-MSC. In line with these results the concentration of IL-6, IL-10 and CXCL8 was increased in the supernatant of the DC-MSCl co-cultures, while the secretion of TNF-?, CXCL10, IL-12 and IFN? was reduced. Furthermore, the concerted action of mechanisms involved in the regulation of DC migration resulted in the blockade of cell migration indicating altered DC functionality mediated by MSCl cell-derived signals and mechanisms resulting in a suppressive microenvironment.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
mesenchymal stromal cell
dendritic cell
immunsuppression
RIG-like receptors
matrix metalloproteinases
Megjelenés:Stem Cells And Development. - 24 : 15 (2015), p. 1805-1816. -
További szerzők:Türk-Mázló Anett (1989-) (molekuláris biológus) Kis-Tóth Katalin (1975-) (immunológus) Szabó Attila (1981-) (molekuláris biológus, immunológus, filozófus) Panyi György (1966-) (biofizikus) Sarkadi Balázs Apáti Ágota Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:TÁMOP 4.2.4. A/2-11-1-2012-0001
TÁMOP
TÁMOP 4.2.2.A-11/1/KONV-2012-0023
TÁMOP
OTKA NK 101538
OTKA
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Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM101422
035-os BibID:(cikkazonosító)1333 (WoS)000785168700001 (Scopus)85128231955
Első szerző:Tarban, Nastaran (molekuláris biológus)
Cím:Regenerating Skeletal Muscle Compensates for the Impaired Macrophage Functions Leading to Normal Muscle Repair in Retinol Saturase Null Mice / Tarban Nastaran, Halász Hajnalka, Gogolák Péter, Garabuczi Éva, Moise Alexander R., Palczewski Krzysztof, Sarang Zsolt, Szondy Zsuzsa
Dátum:2022
ISSN:2073-4409
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Cells. - 11 : 8 (2022), p. 1-16. -
További szerzők:Halász Hajnalka Emese (1994-) (Molekuláris biológus) Gogolák Péter (1968-) (biológus, immunológus) Garabuczi Éva (1982-) (biokémikus, molekuláris biológus) Moise, Alexander R. Palczewski, Krzysztof Sarang Zsolt (1976-) (mikrobiológus) Szondy Zsuzsanna (1959-) (molekuláris sejtbiológus, biokémikus)
Pályázati támogatás:GINOP-2.3.2-15-2016-00006
GINOP
NKFIH 124244
Egyéb
NKFIH138162
Egyéb
NKFIH 125477
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM088858
035-os BibID:(cikkazonosító)8847038 (WoS)000591736500001 (Scopus)85096040529
Első szerző:Veréb Zoltán (immunológus, mikrobiológus, molekuláris biológus)
Cím:Vessel Wall-Derived Mesenchymal Stromal Cells Share Similar Differentiation Potential and Immunomodulatory Properties with Bone Marrow-Derived Stromal Cells / Veréb Zoltán, Mázló Anett, Szabó Attila, Póliska Szilárd, Kiss Attila, Litauszky Krisztina, Koncz Gábor, Boda Zoltán, Rajnavölgyi Éva, Bácsi Attila
Dátum:2020
ISSN:1687-966X 1687-9678
Megjegyzések:This study is aimed at investigating the phenotype, differentiation potential, immunomodulatory properties, and responsivenessof saphenous vein vessel wall-derived mesenchymal stromal cells (SV-MSCs) to various TLR ligands and proinflammatory cytokines, aswell as comparing their features to those of theirbone marrow-derived counterparts (BM-MSCs).Methods. SV-MSCs were isolated byenzymatic digestion of the saphenous vein vessel wall. Phenotype analysis was carried out byflow cytometry and microscopy, whereasadipogenic, chondrogenic, and osteogenic differentiation potentials were tested inin vitroassays. For comparative analysis, theexpression of different stemness, proliferation, and differentiation-related genes was determined by Affymetrix gene array. To comparethe immunomodulatory properties of SV-MSCs and BM-MSCs, mixed lymphocyte reaction was applied. To investigate theirresponses to various activating stimuli, MSCs weretreated with TLR ligands (LPS, PolyI:C) or proinflammatory cytokines (TNF?,IL-1?,IFN?), and the expression of various early innate immune response-related genes was assessed by qPCR, while secretion ofselected cytokines and chemokines was measured by ELISA.Results. The isolated SV-MSCs were able to differentiate into bone, fat,and cartilage cells/directionin vitro. SV-MSCs expressed the most important MSC markers (CD29, CD44, CD73, CD90, and CD105)and shared almost identical phenotypic characteristics with BM-MSCs. Their gene expression pattern and activation pathways wereclose to those of BM-MSCs. SV-MSCs showed better immunosuppressive activity inhibiting phytohemagglutinin-induced Tlymphocyte proliferationin vitrothan BM-MSCs. Cellular responses to treatments mimicking inflammatory conditions werecomparable in the bone marrow- and saphenous vein-derived MSCs. Namely, similar to BM-MSCs, SV-MSCs secreted increasedamount of IL-6 and IL-8 after 12- or 24-hour treatment with LPS, PolyI:C, TNF?,orIL-1?,comparedtountreatedcontrols.Interestingly, a different CXCL-10/IP-10 secretion pattern could be observed under inflammatory conditions in the two types ofMSCs.Conclusion. Based on our results, cells isolated from saphenous vein vessel wall fulfilled the ISCT's (International Society forCellular Therapy) criteria for multipotent mesenchymal stromal cells, and no significant differences in the phenotype, gene expressionpattern, and responsiveness to inflammatory stimuli could be observed between BM-MSCs and SV-MSCs, while the latter cells havemore potent immunosuppressive activityin vitro. Further functional assays have to be performed to reveal whether SV-MSCs couldbe useful for certain regenerative therapeutic applications or tissueengineering purposes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Stem Cells International. - 2020 (2020), p. 1-16. -
További szerzők:Türk-Mázló Anett (1989-) (molekuláris biológus) Szabó Attila (1981-) (molekuláris biológus, immunológus, filozófus) Póliska Szilárd (1978-) (biológus) Kiss Attila (1942-) (belgyógyász, haematológus) Litauszky Krisztina (1967-) (orvos) Koncz Gábor (1970-) (biológus, immunológus) Boda Zoltán (1947-) (belgyógyász, haematologus, klinikai onkológus) Rajnavölgyi Éva (1950-) (immunológus) Bácsi Attila (1967-) (immunológus)
Pályázati támogatás:GINOP-2.3.2-15-2016-00050
GINOP
OTKA-114423
OTKA
NKFIH K 125337
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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