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001-es BibID:	BIBFORM028807
Első szerző:	Antal Miklós (orvos, anatómus)
Cím:	The application of cobalt labelling to electron microscopic investigations of serial sections / Antal M.
Dátum:	1984
ISSN:	0165-0270
Megjegyzések:	The cobalt labelling technique can be applied to ultrathin serial sections and subsequent electron microscopical investigations with the following modifications: a prolonged, up to 12 h, fixation of the tissue in aldehydes; a shortened, 15 min, postfixation in OsO4; embedding in soft resin block by using a higher proportion of plasticizer in the polimerizing mixture; mounting of 5 micrometers thick serial sections between two layers of Agar-Agar coatings; performing the intensification of the Agar section-Agar sandwich with a physical developer containing a low percentage of the reductive agent; reembedding selected thick sections for ultrathin serial sectioning and staining with uranile acetate and lead citrate. The technique unambiguously shows all labelled profiles, and preserves the fine structural details of the surrounding tissues.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal Of Neuroscience Methods. - 12 : 1 (1984), p. 69-77. -
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM029487
Első szerző:	Wolf Ervin (fizikus, neurobiológus)
Cím:	A fast 3-dimensional neuronal tree reconstruction system that uses cubic polynomials to estimate dendritic curvature / Wolf E., Birinyi A., Pomahazi S.
Dátum:	1995
ISSN:	0165-0270
Megjegyzések:	The main goal of this work was to develop and test the accuracy of our 3DARBOR neuronal tree reconstruction system by comparing it with a very precise but time-consuming method of reconstruction (NEUTRACE). Comparison was performed by reconstructing 18 dendritic trees of frog spinal motoneurons from serial sections with both methods and comparing several morphological summaries of the two reconstructions. In 3DARBOR the planar projection of the dendritic trees was drawn and fed into an IBM-compatible PC through a graphic tablet. Dendritic coordinates along the perpendicular (focus) axis on the plane of drawing were estimated by an interpolation method. The interpolation was based on the lengths of projected dendrites and the coordinates of points where dendrites entered the next section. Focus axis coordinates of these points could automatically be calculated from the serial numbers and thicknesses of sections. 3DARBOR was tested by comparison of the distributions of characteristic points of dendritic trees, segment lengths and branching angles. Product moment analysis on dendritic trees was also performed. It was concluded that 3DARBOR is a fast enough reconstruction system without any systematical error of interpolation that can correctly supply the most morphological parameters and visualize the natural arborizations.
Tárgyszavak:	Orvostudományok Természettudományok Biológiai tudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal Of Neuroscience Methods. - 63 : 1-2 (1995), p. 137-145. -
További szerzők:	Birinyi András (1960-) (anatómus, neurobiológus) Pomaházi Sándor
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