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001-es BibID:	BIBFORM028807
Első szerző:	Antal Miklós (orvos, anatómus)
Cím:	The application of cobalt labelling to electron microscopic investigations of serial sections / Antal M.
Dátum:	1984
ISSN:	0165-0270
Megjegyzések:	The cobalt labelling technique can be applied to ultrathin serial sections and subsequent electron microscopical investigations with the following modifications: a prolonged, up to 12 h, fixation of the tissue in aldehydes; a shortened, 15 min, postfixation in OsO4; embedding in soft resin block by using a higher proportion of plasticizer in the polimerizing mixture; mounting of 5 micrometers thick serial sections between two layers of Agar-Agar coatings; performing the intensification of the Agar section-Agar sandwich with a physical developer containing a low percentage of the reductive agent; reembedding selected thick sections for ultrathin serial sectioning and staining with uranile acetate and lead citrate. The technique unambiguously shows all labelled profiles, and preserves the fine structural details of the surrounding tissues.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal Of Neuroscience Methods. - 12 : 1 (1984), p. 69-77. -
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM071095
Első szerző:	Richardson, Stephen M.
Cím:	Mesenchymal stem cells in regenerative medicine : focus on articular cartilage and intervertebral disc regeneration / Richardson Stephen M., Kalamegam Gauthaman, Pushparaj Peter N., Matta Csaba, Memic Adnan, Khademhosseini Ali, Mobasheri Reza, Poletti Fabian L., Hoyland Judith A., Mobasheri Ali
Dátum:	2016
ISSN:	1046-2023
Megjegyzések:	Musculoskeletal disorders represent a major cause of disability and morbidity globally and result in enormous costs for health and social care systems. Development of cell-based therapies is rapidly proliferating in a number of disease areas, including musculoskeletal disorders. Novel biological therapies that can effectively treat joint and spine degeneration are high priorities in regenerative medicine. Mesenchymal stem cells (MSCs) isolated from bone marrow (BM-MSCs), adipose tissue (AD-MSCs) and umbilical cord (UC-MSCs) show considerable promise for use in cartilage and intervertebral disc (IVD) repair. This review article focuses on stem cell-based therapeutics for cartilage and IVD repair in the context of the rising global burden of musculoskeletal disorders. We discuss the biology MSCs and chondroprogenitor cells and specifically focus on umbilical cord/Wharton's jelly derived MSCs and examine their potential for regenerative applications. We also summarize key components of the molecular machinery and signaling pathways responsible for the control of chondrogenesis and explore biomimetic scaffolds and biomaterials for articular cartilage and IVD regeneration. This review explores the exciting opportunities afforded by MSCs and discusses the challenges associated with cartilage and IVD repair and regeneration. There are still many technical challenges associated with isolating, expanding, differentiating, and pre-conditioning MSCs for subsequent implantation into degenerate joints and the spine. However, the prospect of combining biomaterials and cell-based therapies that incorporate chondrocytes, chondroprogenitors and MSCs leads to the optimistic view that interdisciplinary approaches will lead to significant breakthroughs in regenerating musculoskeletal tissues, such as the joint and the spine in the near future.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Methods 99 (2016), p. 69-80. -
További szerzők:	Kalamegam, Gauthaman Pushparaj, Peter N. Matta Csaba (1980-) (molekuláris biológus, genetikus, angol szakfordító) Memic, Adnan Khademhosseini, Ali Mobasheri, Reza Poletti, Fabian L. Hoyland, Judith A. Mobasheri, Ali
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001-es BibID:	BIBFORM088736
035-os BibID:	(cikkazonosító)112877
Első szerző:	Szabó Gábor (PhD-hallgató)
Cím:	Distinct and overlapping effects of [beta]2-glycoprotein I conformational variants in ligand interactions and functional assays / Szabó Gábor, Pénzes Krisztina, Torner Bernadett, Fagyas Miklós, Tarr Tünde, Soltész Pál, Kis Gréta, Antal Miklós, Kappelmayer János
Dátum:	2020
ISSN:	0022-1759
Megjegyzések:	One of the most abundant coagulation proteins is ?2-glycoprotein I (?2GPI) that is present in humans at a concentration of around 200 mg/L. Its physiological role is only partially understood, but it adopts several different structural forms the majority of which are the open and closed forms. We isolated native (circular) ?2GPI and converted it into an open conformation. The effectiveness of these procedures was assessed by Western blot and negative-staining electron microscopy. We found that in coagulation assays the open form of ?2GPI had a significant prolonging effect on fibrin formation in a dilute prothrombin time test (p < 0.001). In the dilute activated partial thromboplastin time test, both conformations had a significant prolonging effect (p < 0.001) but the open conformation was more effective. In a fluorescent thrombin generation assay both conformations slightly delayed thrombin generation with no significant effect on the quantity of formed thrombin. By using surface plasmon resonance assays, the equilibrium dissociation constants of both the open and closed conformations of ?2GPI showed a similar and strong affinity to isolated anti-?2GPI autoantibodies (Kd closed ?2GPI = 5.17 ? 10-8 M, Kd open ?2GPI = 5.56 ? 10-8 M) and the open form had one order of magnitude stronger affinity to heparin (Kd = 0.30 ? 10-6 M) compared to the closed conformation (Kd = 3.50 ? 10-6 M). The two different forms of ?2GPI have distinct effects in functional tests and in ligand binding, which may considerably affect the intravascular events related to this abundant plasma protein in health and disease.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Anti-[beta](2)-glycoprotein I Heparin Surface plasmon resonance Thrombin generation [beta](2)-glycoprotein I conformations
Megjelenés:	Journal of Immunological Methods. - 487 (2020), p. 112877. -
További szerzők:	Pénzes-Daku Krisztina (1978-) (biológus) Torner Bernadett (1997-) (molekuláris biológus) Fagyas Miklós (1984-) (orvos) Tarr Tünde (1976-) (belgyógyász, allergológus és klinikai immunológus) Soltész Pál (1961-) (belgyógyász, kardiológus) Kis Gréta (1979-) (molekuláris biológus) Antal Miklós (1951-) (orvos, anatómus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Pályázati támogatás:	OTKA K16 120725 OTKA GINOP-2.3.2-15-2016-00043 GINOP
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001-es BibID:	BIBFORM029487
Első szerző:	Wolf Ervin (fizikus, neurobiológus)
Cím:	A fast 3-dimensional neuronal tree reconstruction system that uses cubic polynomials to estimate dendritic curvature / Wolf E., Birinyi A., Pomahazi S.
Dátum:	1995
ISSN:	0165-0270
Megjegyzések:	The main goal of this work was to develop and test the accuracy of our 3DARBOR neuronal tree reconstruction system by comparing it with a very precise but time-consuming method of reconstruction (NEUTRACE). Comparison was performed by reconstructing 18 dendritic trees of frog spinal motoneurons from serial sections with both methods and comparing several morphological summaries of the two reconstructions. In 3DARBOR the planar projection of the dendritic trees was drawn and fed into an IBM-compatible PC through a graphic tablet. Dendritic coordinates along the perpendicular (focus) axis on the plane of drawing were estimated by an interpolation method. The interpolation was based on the lengths of projected dendrites and the coordinates of points where dendrites entered the next section. Focus axis coordinates of these points could automatically be calculated from the serial numbers and thicknesses of sections. 3DARBOR was tested by comparison of the distributions of characteristic points of dendritic trees, segment lengths and branching angles. Product moment analysis on dendritic trees was also performed. It was concluded that 3DARBOR is a fast enough reconstruction system without any systematical error of interpolation that can correctly supply the most morphological parameters and visualize the natural arborizations.
Tárgyszavak:	Orvostudományok Természettudományok Biológiai tudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal Of Neuroscience Methods. - 63 : 1-2 (1995), p. 137-145. -
További szerzők:	Birinyi András (1960-) (anatómus, neurobiológus) Pomaházi Sándor
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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