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001-es BibID:	BIBFORM038137
Első szerző:	Balogh István (molekuláris biológus, genetikus)
Cím:	Analysis of Gas6 in human platelets and plasma / Istvan Balogh, Sassan Hafizi, Jonas Stenhoff, Karin Hansson, Björn Dahlback
Dátum:	2005
ISSN:	1079-5642
Megjegyzések:	OBJECTIVE: Gas6 is a member of the vitamin K-dependent protein family. Gas6-deficient mice were found to be resistant to thrombosis because of defective platelet function. Mouse Gas6 was demonstrated to be present in platelets and found to be involved in platelet aggregation. The aim of this study was to investigate the presence of Gas6 in human platelets and plasma and determine its role in platelet function. METHODS AND RESULTS: The presence of Gas6 in human platelets and plasma was analyzed using sensitive immunologic methods. Mass spectrometry and ELISA were used to identify and quantify Gas6 in plasma. Gas6 was demonstrated to be present in human plasma, at a concentration determined to be 13 to 23 ng/mL (0.16 to 0.28 nM). Furthermore, plasma Gas6 levels were found to be lower in patients administered with warfarin. However, Gas6 was undetectable in human platelets. CONCLUSIONS: This is the first report to identify and quantify Gas6 in human plasma. However, Gas6 protein was not detected in human platelets, suggesting that any potential platelet-specific function could be because of Gas6 from the circulation. These findings open up new directions regarding the role of Gas6 in normal and pathophysiological situations such as inflammation, autoimmune disease, thrombosis and arteriosclerosis.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Arteriosclerosis Thrombosis And Vascular Biology. - 25 : 6 (2005), p. 1280-1286. -
További szerzők:	Hafizi, Sassan Stenhoff, Jonas Hansson, Karin Dahlbäck, Björn
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001-es BibID:	BIBFORM043579
Első szerző:	Cauwenberghs, Nancy
Cím:	Antithrombotic effect of platelet glycoprotein Ib-blocking monoclonal antibody Fab fragments in nonhuman primates / Cauwenberghs N., Meiring M., Vauterin S., van Wyk V., Lamprecht S., Roodt J. P., Novák L., Harsfalvi J., Deckmyn H., Kotzé H. F.
Dátum:	2000
Megjegyzések:	Platelet adhesion in arterial blood flow is mainly supported by the platelet receptor glycoprotein (GP) Ib, which interacts with von Willebrand factor (vWF) that is bound to collagen at the site of vessel wall injury. Antibody 6B4 is a monoclonal antibody (MoAb) raised against purified human GPIb. MoAb 6B4 inhibits both ristocetin- and botrocetin-induced, vWF-dependent human platelet agglutination. MoAb 6B4 furthermore blocks shear-induced adhesion of human platelets to collagen I. We studied the antithrombotic effect of this inhibitory murine MoAb 6B4 in a baboon model of arterial thrombosis. When injected into baboons, intact IgG and its F(ab')(2) fragments caused almost immediate thrombocytopenia, whereas injection of the Fab fragments alone did not. Fab fragments were subsequently used to investigate their in vivo effect on platelet deposition onto a thrombogenic device, consisting of collagen-rich, glutaraldehyde-fixed bovine pericardium (0.6 cm(2)), at a wall shear rate ranging from 700 to 1000 s(-1). Baboons were either pretreated with Fabs to study the effect of inhibition on platelet adhesion or treated 6 minutes after placement of the thrombogenic device to investigate the effect on interplatelet cohesion. Pretreatment of the animals with bolus doses ranging from 80 to 640 microgram/kg Fab fragments significantly reduced (111)In-labeled platelet deposition onto the collagen surface by approximately 43% to 65%. Only the highest dose caused a significant prolongation (doubling) of the bleeding time. Ex vivo ristocetin-induced platelet agglutination was equally reduced. Treatment with a bolus of 110 microgram/kg Fab fragments after a thrombus was allowed to form for 6 minutes had no effect on further platelet deposition. We therefore conclude that Fab fragments or derivatives of inhibitory anti-GPIb antibodies may be useful compounds to prevent thrombosis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban platelet adhesion platelet aggregation thrombosis glycoprotein Ib monoclonal antibodies
Megjelenés:	Arteriosclerosis, Thrombosis, and Vascular Biology. - 20 : 5 (2000), p. 1347-1353. -
További szerzők:	Meiring, Muriel Vauterin, Stephan van Wyk, Veronika Lamprecht, Seb Roodt, Jan P. Novák Levente (1967-) (biológus) Hársfalvi Jolán (1949-) (klinikai biokémikus) Deckmyn, Hans Kotzé, Harry F.
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001-es BibID:	BIBFORM010055
Első szerző:	Chari, Ramya
Cím:	Protein kinase C[delta] differentially regulates platelet functional responses / Chari R., Getz T., Nagy B., Jr., Bhavaraju K., Mao Y., Bynagari Y. S., Murugappan S., Nakayama K., Kunapuli S. P.
Dátum:	2009
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Arteriosclerosis, Thrombosis, and Vascular Biology. - 29 : 5 (2009), p. 699-705. -
További szerzők:	Getz, Todd Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Bhavaraju, Kamala Mao, Yingying Bynagari, Yamini Saraswathy Murugappan, Swaminathan Nakayama, Keiko Kunapuli, Satya P.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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