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001-es BibID:	BIBFORM096191
035-os BibID:	(cikkazonosító)9499 (scopus)85114049778 (wos)000694373200001
Első szerző:	Dienes Csaba (gyógyszerész)
Cím:	Electrophysiological Effects of the Transient Receptor Potential Melastatin 4 Channel Inhibitor (4-Chloro-2-(2-chlorophenoxy)acetamido) Benzoic Acid (CBA) in Canine Left Ventricular Cardiomyocytes / Csaba Dienes, Tamás Hézső, Dénes Zsolt Kiss, Dóra Baranyai, Zsigmond Máté Kovács, László Szabó, János Magyar, Tamás Bányász, Péter P. Nánási, Balázs Horváth, Mónika Gönczi, Norbert Szentandrássy
Dátum:	2021
ISSN:	1661-6596 1422-0067
Megjegyzések:	Transient receptor potential melastatin 4 (TRPM4) plays an important role in many tissues, including pacemaker and conductive tissues of the heart, but much less is known about its electrophysiological role in ventricular myocytes. Our earlier results showed the lack of selectivity of 9-phenanthrol, so CBA ((4-chloro-2-(2-chlorophenoxy)acetamido) benzoic acid) was chosen as a new, potentially selective inhibitor. Goal: Our aim was to elucidate the effect and selectivity of CBA in canine left ventricular cardiomyocytes and to study the expression of TRPM4 in the canine heart. Experiments were carried out in enzymatically isolated canine left ventricular cardiomyocytes. Ionic currents were recorded with an action potential (AP) voltage-clamp technique in whole-cell configuration at 37 ?C. An amount of 10 mM BAPTA was used in the pipette solution to exclude the potential activation of TRPM4 channels. AP was recorded with conventional sharp microelectrodes. CBA was used in 10 ?M concentrations. Expression of TRPM4 protein in the heart was studied by Western blot. TRPM4 protein was expressed in the wall of all four chambers of the canine heart as well as in samples prepared from isolated left ventricular cells. CBA induced an approximately 9% reduction in AP duration measured at 75 and 90% of repolarization and decreased the short-term variability of APD90. Moreover, AP amplitude was increased and the maximal rates of phase 0 and 1 were reduced by the drug. In AP clamp measurements, CBA-sensitive current contained a short, early outward and mainly a long, inward current. Transient outward potassium current (Ito) and late sodium current (INa,L) were reduced by approximately 20 and 47%, respectively, in the presence of CBA, while L-type calcium and inward rectifier potassium currents were not affected. These effects of CBA were largely reversible upon washout. Based on our results, the CBA induced reduction of phase-1 slope and the slight increase of AP amplitude could have been due to the inhibition of Ito. The tendency for AP shortening can be explained by the inhibition of inward currents seen in AP-clamp recordings during the plateau phase. This inward current reduced by CBA is possibly INa,L, therefore, CBA is not entirely selective for TRPM4 channels. As a consequence, similarly to 9-phenanthrol, it cannot be used to test the contribution of TRPM4 channels to cardiac electrophysiology in ventricular cells, or at least caution must be applied.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk CBA TRPM4 cardiac ionic currents cardiac action potentials canine myocytes action potential voltage clamp
Megjelenés:	International Journal Of Molecular Sciences. - 22 : 17 (2021), p. 9499. -
További szerzők:	Hézső Tamás (1993-) (élettanász) Kiss Dénes Zsolt (1995-) (orvos, élettanász) Baranyai Dóra Kovács Zsigmond Máté (1995-) (orvos) Szabó László (1994-) (molekuláris biológus) Magyar János (1961-) (élettanász) Bányász Tamás (1960-) (élettanász) Nánási Péter Pál (1956-) (élettanász) Horváth Balázs (1981-) (élettanász) Gönczi Mónika (1974-) (élettanász) Szentandrássy Norbert (1976-) (élettanász)
Pályázati támogatás:	NKFIH-K115397 Egyéb NKFIH-K138090 Egyéb NKFIH-FK128116 Egyéb GINOP-2.3.2-15-2016-00040 GINOP EFOP-3.6.2-16-2017-00006 EFOP DE-SPACE(TKP-2020-NKA-04) Egyéb EFOP-3.6.3-VEKOP-16-2017-00009 EFOP ÚNKP-20-3 Egyéb ÚNKP-20-2 Egyéb
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001-es BibID:	BIBFORM099802
035-os BibID:	(cikkazonosító)917 (scopus)85122782091 (wos)000757677800001
Első szerző:	Gönczi Mónika (élettanász)
Cím:	Astaxanthin Exerts Anabolic Effects via Pleiotropic Modulation of the Excitable Tissue / Gönczi Mónika, Csemer Andrea, Szabó László, Sztretye Mónika, Fodor János, Pocsai Krisztina, Szenthe Kálmán, Keller-Pintér Anikó, Köhler Zoltán Márton, Nánási Péter, Szentandrássy Norbert, Pál Balázs, Csernoch László
Dátum:	2022
ISSN:	1661-6596 1422-0067
Megjegyzések:	Astaxanthin is a lipid-soluble carotenoid influencing lipid metabolism, body weight, and insulin sensitivity. We provide a systematic analysis of acute and chronic effects of astaxanthin on different organs. Changes by chronic astaxanthin feeding were analyzed on general metabolism, expression of regulatory proteins in the skeletal muscle, as well as changes of excitation and synaptic activity in the hypothalamic arcuate nucleus of mice. Acute responses were also tested on canine cardiac muscle and different neuronal populations of the hypothalamic arcuate nucleus in mice. Dietary astaxanthin significantly increased food intake. It also increased protein levels affecting glucose metabolism and fatty acid biosynthesis in skeletal muscle. Inhibitory inputs innervating neurons of the arcuate nucleus regulating metabolism and food intake were strengthened by both acute and chronic astaxanthin treatment. Astaxanthin moderately shortened cardiac action potentials, depressed their plateau potential, and reduced the maximal rate of depolarization. Based on its complex actions on metabolism and food intake, our data support the previous findings that astaxanthin is suitable for supplementing the diet of patients with disturbances in energy homeostasis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk astaxanthin metabolism food intake gene expression skeletal muscle cardiac action potential arcuate nucleus excitability inhibitory postsynaptic current
Megjelenés:	International Journal Of Molecular Sciences. - 23 : 2 (2022), p. 917. -
További szerzők:	Csemer Andrea (1994-) (molekuláris biológus) Szabó László (1994-) (molekuláris biológus) Sztretye Mónika (1981-) (élettanász, elektrofiziológus) Fodor János (1973-) (élettanász, biotechnológus) Pocsai Krisztina (1978-) (élettanász) Szenthe Kálmán Keller-Pintér Anikó Köhler Zoltán Márton Nánási Péter Pál (1956-) (élettanász) Szentandrássy Norbert (1976-) (élettanász) Pál Balázs (1975-) (élettanász) Csernoch László (1961-) (élettanász)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00040 GINOP GINOP-2.3.3-15-2016-00020 GINOP NKFI FK 134684 Egyéb NKFIH PD-128370 Egyéb OTKA-115397 OTKA NKFIH K 138090 Egyéb EFOP-3.6.3-VEKOP-16-2017-00009 EFOP EFOP-3.6.2-16-2017-00006 EFOP TKP2020-IKA-04 Egyéb 2020-4.1.1-TKP2020 Egyéb TKP-2020-NKA-04 Egyéb
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001-es BibID:	BIBFORM105581
035-os BibID:	(cikkazonosító)14651 (WOS)000897324400001 (Scopus)85143732443 (PubMed)36498977
Első szerző:	Nagy Norbert (kísérletes farmakológus)
Cím:	Antiarrhythmic and Inotropic Effects of Selective Na+/Ca2+ Exchanger Inhibition : What Can We Learn from the Pharmacological Studies? / Nagy Norbert, Tóth Noémi, Nánási Péter P.
Dátum:	2022
ISSN:	1422-0067
Megjegyzések:	Life-long stable heart function requires a critical balance of intracellular Ca2+. Several ion channels and pumps cooperate in a complex machinery that controls the influx, release, and efflux of Ca2+. Probably one of the most interesting and most complex players of this crosstalk is the Na+/Ca2+ exchanger, which represents the main Ca2+ efflux mechanism; however, under some circumstances, it can also bring Ca2+ into the cell. Therefore, the inhibition of the Na+/Ca2+ exchanger has emerged as one of the most promising possible pharmacological targets to increase Ca2+ levels, to decrease arrhythmogenic depolarizations, and to reduce excessive Ca2+ influx. In line with this, as a response to increasing demand, several more or less selective Na+/Ca2+ exchanger inhibitor compounds have been developed. In the past 20 years, several results have been published regarding the effect of Na+/Ca2+ exchanger inhibition under various circumstances, e.g., species, inhibitor compounds, and experimental conditions; however, the results are often controversial. Does selective Na+/Ca2+ exchanger inhibition have any future in clinical pharmacological practice? In this review, the experimental results of Na+/Ca2+ exchanger inhibition are summarized focusing on the data obtained by novel highly selective inhibitors.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk heart Na+/Ca2+ exchanger arrhythmia Ca2+-handling inotropy
Megjelenés:	International Journal Of Molecular Sciences. - 23 : 23 (2022), p. 14651. -
További szerzők:	Tóth Noémi Nánási Péter Pál (1956-) (élettanász)
Pályázati támogatás:	FK-129117 OTKA
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001-es BibID:	BIBFORM106377
035-os BibID:	(cikkazonosító)446 (WOS)000910182000001 (Scopus)85145970899
Első szerző:	Ráduly Arnold Péter
Cím:	The Novel Cardiac Myosin Activator Danicamtiv Improves Cardiac Systolic Function at the Expense of Diastolic Dysfunction In Vitro and In Vivo : Implications for Clinical Applications / Ráduly Arnold Péter, Sárkány Fruzsina, Kovács Máté Balázs, Bernát Brigitta, Juhász Béla, Szilvássy Zoltán, Porszász Róbert, Horváth Balázs, Szentandrássy Norbert, Nánási Péter, Csanádi Zoltán, Édes István, Tóth Attila, Papp Zoltán, Priksz Dániel, Borbély Attila
Dátum:	2023
ISSN:	1422-0067
Megjegyzések:	Recent cardiotropic drug developments have focused on cardiac myofilaments. Danicamtiv, the second direct myosin activator, has achieved encouraging results in preclinical and clinical studies, thus implicating its potential applicability in the treatment of heart failure with reduced ejection fraction (HFrEF). Here, we analyzed the inotropic effects of danicamtiv in detail. To this end, changes in sarcomere length and intracellular Ca2+ levels were monitored in parallel, in enzymatically isolated canine cardiomyocytes, and detailed echocardiographic examinations were performed in anesthetized rats in the absence or presence of danicamtiv. The systolic and diastolic sarcomere lengths decreased; contraction and relaxation kinetics slowed down with increasing danicamtiv concentrations without changes in intracellular Ca2+ transients in vitro. Danicamtiv evoked remarkable increases in left ventricular ejection fraction and fractional shortening, also reflected by changes in systolic strain. Nevertheless, the systolic ejection time was significantly prolonged, the ratio of diastolic to systolic duration was reduced, and signs of diastolic dysfunction were also observed upon danicamtiv treatment in vivo. Taken together, danicamtiv improves cardiac systolic function, but it can also limit diastolic performance, especially at high drug concentrations.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk heart failure with reduced ejection fraction myosin activators danicamtiv positive inotropy strain diastolic dysfunction
Megjelenés:	International Journal Of Molecular Sciences. - 24 : 1 (2023), p. 446. -
További szerzők:	Sárkány Fruzsina (1996-) (orvos) Kovács Máté Balázs Bernát Brigitta (1997-) (farmakológus) Juhász Béla (1978-) (kísérletes farmakológus) Szilvássy Zoltán (1957-) (belgyógyász, farmakológus, klinikai farmakológus) Pórszász Róbert (1965-) (farmakológus, klinikai farmakológus) Horváth Balázs (1981-) (élettanász) Szentandrássy Norbert (1976-) (élettanász) Nánási Péter Pál (1956-) (élettanász) Csanádi Zoltán (1960-) (kardiológus) Édes István (1952-) (kardiológus) Tóth Attila (1971-) (biológus) Papp Zoltán (1965-) (kardiológus, élettanász) Priksz Dániel (1989-) (farmakológus) Borbély Attila (1978-) (kardiológus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00043 GINOP ÚNKP-21-3 Egyéb TKP2020-IKA-04 Egyéb TKP2020-NKA-04 Egyéb 2020-4.1.1-TKP2020 Egyéb NFKIH - K 132623 Egyéb NKFIH-K138090 Egyéb NKFIH-K142764 Egyéb NKFIH-FK128116 Egyéb
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