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001-es BibID:BIBFORM035804
Első szerző:Bánhegyi Dénes
Cím:Significant decrease of the enhancement/neutralization index in HIV patients during highly active antiretroviral therapy (HAART) / Bánhegyi Dénes, Bácsi Attila, Tóth Ferenc D., Prohászka Zoltán, Horváth Anna, Beck Zoltán, Kónya József, Füst George
Dátum:2003
ISSN:0165-2478
Megjegyzések:Authors studied the effect of highly active antiretroviral therapy (HAART) on balance of the antibodies that enhance or neutralize growth of HIV-1(IIIB) strain in MT-4 cells in the presence or absence of human complement. Sequential serum samples were collected from 28 patients in advanced stage of HIV disease before and during HAART. The balance of the enhancing and neutralizing antibodies was expressed by an index value (E/N I). Samples with an E/N I of <0.5 (twofold decrease in virus production) were considered as neutralizing, whereas samples with an E/N I>2.0 (twofold increase in virus production) were considered as enhancing. At the beginning of HAART serum samples from eight patients enhanced, and samples from only two patients neutralized the virus in the presence of complement, median (25th-75th percentile) value of E/N I was 1.32 (0.79-2.29). E/N I significantly (P<0.0001) dropped to 0.37 (0.19-0.57) during the follow-up period of 18.5 (10.5-23.5) months under HAART. Similar changes were detected when serum samples were tested with no complement added. The E/N I values were also markedly decreased when cultures inoculated with mixtures of HIV and purified IgG prepared from serum pools taken before and during HAART, respectively, were compared. In the last samples of 20/28 patients, neutralization was measured even in the presence of complement while enhancement was found with none of these samples. These findings suggest that HAART results in disappearance of enhancing antibodies and switches the E/N I toward neutralization.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Immunology Letters. - 89 : 1 (2003), p. 25-30. -
További szerzők:Bácsi Attila (1967-) (immunológus) Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász) Prohászka Zoltán Horváth Anna Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Füst György (Budapest)
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM055858
Első szerző:Szabó Judit (szakorvos, klinikai mikrobiológus)
Cím:Studies on the susceptibility of natural killer cells to HIV-1 strains of various tropism / Szabó Judit, Beck Zoltán, Bácsi Attila, D. Tóth Ferenc
Dátum:2000
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Immunology Letters. - 73 : 2-3 (2000), p. 181. -
További szerzők:Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Bácsi Attila (1967-) (immunológus) Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
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3.

001-es BibID:BIBFORM037557
Első szerző:Vida András (molekuláris biológus, genetikus)
Cím:Fusion of the Fc part of human IgG1 to CD14 enhances its binding to gram-negative bacteria and mediates phagocytosis by Fc receptors of neutrophils / András Vida, Bart Bardoel, Fin Milder, László Majoros, Andrea Sümegi, Attila Bácsi, György Vereb, Kok P. M. van Kessel, Jos A. G. van Strijp, Péter Antal-Szalmás
Dátum:2012
ISSN:0165-2478
Megjegyzések:Microbial resistance to antimicrobial drugs is promoting a search for new antimicrobial agents that target highly conservative structures of pathogens. Human CD14 a known pattern recognition receptor (PRR) which recognizes multiple ligands from different microbes might be a worthy candidate. The aim of our work was to create a CD14/Fc dimer protein and evaluate its whole bacteria binding and opsonizing capabilities. Fusion of CD14 with the fragment crystallisable (Fc) part of human IgG1 could not only lead to an artificial opsonin but the dimerization through the Fc part might also increase its affinity to different ligands. Human CD14 and the Fc part of human IgG1 was fused and expressed in HEK293 cells. A histidine tagged CD14 (CD14/His) was also expressed as control. Using flow cytometry we could prove that CD14/Fc bound to whole Gram-negative bacteria, especially to short lipopolysaccharide (Ra and Re) mutants, and weak interaction was observed between the fusion protein and Listeria monocytogenes. Other Gram-positive bacteria and fungi did not show any association with CD14/Fc. CD14/His showed about 50-times less potent binding to Gram-negative bacteria. CD14/Fc acted as an opsonin and enhanced phagocytosis of these bacteria by neutrophil granulocytes, monocyte-derived macrophages and dendritic cells. Internalization of bacteria was confirmed by trypan blue quenching and confocal microscopy. On neutrophils the Fc part of the fusion protein was recognized by Fc receptors (CD16, CD32), as determined by blocking experiments. CD14/Fc enhanced the killing of bacteria in an ex vivo whole blood assay. Our experiments confirm that PRR/Fc fusion proteins can give a boost to FcR dependent phagocytosis and killing provided the antimicrobial part binds efficiently to microbes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
CD14
Fc
Gram-negative bacteria
Opsonization
Phagocytosis
Molekuláris Medicina
Megjelenés:Immunology Letters. - 146 : 1-2 (2012), p. 31-39. -
További szerzők:Bardoel, Bart Milder, Fin Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Sümegi Andrea (1969-) (biológus) Bácsi Attila (1967-) (immunológus) Vereb György (1965-) (biofizikus, orvos) Kessel, Kok P. M., van Strijp, Jos A. G., van Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos)
Pályázati támogatás:T046694
OTKA
TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Celluláris hematológia - immunológia
TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Az oxidatív DNS károsodások javítása és a gyulladás kialakulásának kapcsolata
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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