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001-es BibID:	BIBFORM054788
Első szerző:	Damjanova, Ivelina
Cím:	From farm to fork follow-up of thermotolerant campylobacters throughout the broiler production chain and in human cases in a Hungarian county during a ten-months period / I. Damjanova, M. Jakab, T. Farkas, J. Mészáros, Zs. Galántai, I. Turcsányi, A. Bistyák, Á. Juhász, J. Pászti, I. Kiss, G. Kardos
Dátum:	2011
ISSN:	0168-1605
Tárgyszavak:	Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	International Journal Of Food Microbiology. - 150 : 2-3 (2011), p. 95-102. -
További szerzők:	Jakab M. Farkas T. Mészáros József Galántai Zsuzsanna Turcsányi I. Bistyák Andrea Juhász Á. Pászti Judit Kiss I. Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus)
Pályázati támogatás:	GVOP 3.1.1-2004-0472/3.0 GVOP
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001-es BibID:	BIBFORM004817
Első szerző:	Nógrády Noémi
Cím:	Prevalence and characterization of Salmonella infantis isolates originating from different points of the broiler chicken-human food chain in Hungary / Nógrády Noémi, Kardos Gábor, Bistyák Andrea, Turcsányi Ibolya, Mészáros Júlia, Galántai Zsuzsanna, Juhász Ágnes, Samu Péterné, Kaszanyitzky J. Éva, Pászti Judit, Kiss István
Dátum:	2008
Megjegyzések:	During the 10-month study period Salmonella contamination of broiler houses and the flocks reared in three farms (A, B and C), the slaughter houses where the flocks were slaughtered, as well as the carcass and retail raw meat products originating from them was investigated. In the broiler farm A five consecutive flocks, in the B and C farms one flock was sampled. Environmental samples were taken prior to the introductions. Environmental, drinking water, feed and faecal samples were collected regularly using standard methods. Before and during processing of the flocks, environmental and carcass samples were taken at the abattoirs. Salmonella contamination of the carcass, retail meat, as well as stool samples of farm and abattoir workers and from human illnesses registered in the same period and region were also examined. Isolation, sero-, phage- and antibiotic resistance typing, class 1 integron and plasmid profiling of the strains were performed; their genetic relationship was assessed by PFGE. Although the broiler house and the faecal samples of the 5 flocks of the farm A were negative for Salmonella, S. infantis was isolated from 20?100% of the abattoir carcass samples. The retail raw meat samples were 0?100% S. infantis positive. The environmental samples of farm B were Salmonella negative, but the examined flock was contaminated: S. infantis was identified from 43% of the faecal samples. This serotype was identified in 100% of the carcass and retail raw meat samples. From environmental samples taken before the arrival of the 1-day-old chicks in the broiler house C, S. infantis was cultured. S. infantis prevalence in the faecal samples was 35% and all the carcass and retail raw meat samples were S. infantis contaminated. Altogether 164 S. infantis strains were isolated out of which 145 were further characterized. The vast majority (142/145) of the strains belonged to phage types 217 and 213. All but one were characterized by the nalidixic acid-streptomycin-sulphonamide-tetracycline resistances, had an 885 bp class 1 integron and a large plasmid of > 168 kb in size. The strains showed ? 88.7% genetic similarity. The results obtained shows that the same multi-drug resistant S. infantis clone was spread from the examined broiler farms contaminating the slaughter and the retail meat and appeared in the human illnesses of the examined region that was earlier detected as the dominant clone characteristic of the broiler and human population of the whole country.
Tárgyszavak:	Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk PFGE broiler flocks
Megjelenés:	International Journal of Food Microbiology. - 127 : 1-2 (2008), p. 162-167. -
További szerzők:	Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Bistyák Andrea Turcsányi Ibolya Mészáros Júlia Galántai Zsuzsanna Juhász Ágnes Samu Péter Kaszanyitzky J. Éva Pászti Judit Kiss István (mikrobiológus)
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001-es BibID:	BIBFORM001077
Első szerző:	Peles Ferenc (mikrobiológia, élelmiszer-mikrobiológia, minőségügy)
Cím:	Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary / F. Peles, M. Wagner, L. Varga, I. Hein, P. Rieck, K. Gutser, P. Keresztúri, G. Kardos, I. Turcsányi, B. Béri, A. Szabó
Dátum:	2007
Megjegyzések:	Staphylococcus aureus is a major foodborne pathogen due to its capability to produce a wide range of heat-stable enterotoxins. The primary purpose of this research was to characterize S. aureus isolates recovered from mammary quarter milk of mastitic cows and from bulk tank milk produced on Hungarian dairy farms of different sizes. Macrorestriction analysis of chromosomal DNA from S. aureus isolates was performed using the restriction enzyme SmaI followed by pulsed-field gel electrophoresis (PFGE). The prevalence rates of nine S. aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and of the toxic shock syndrome toxin 1 gene (tst) were determined by multiplex polymerase chain reaction (PCR). The bulk tank milks of 14 out of 20 farms were contaminated with S. aureus at levels of up to 6.0x103 CFU/ml. Farm size had no significant effect (PN0.05) on the S. aureus counts in bulk milk. The prevalence rates of penicillin resistance were 88.9% and 20.0% among the S. aureus recovered from mastitic quarter milk and bulk tank milk, respectively. After phenotypic characterization, a total of 59 S. aureus isolates were selected for genotyping. PFGE analysis revealed 22 distinct pulsotypes, including 14 main types and 8 subtypes, at a similarity level of 86%. Only one or two main types were observed on each of the farms tested, indicating a lack of genetic diversity among S. aureus isolates within farms, and there were only two pulsotypes which occurred on more than one farm. The PFGE patterns showed genetic relatedness between the S. aureus strains recovered from quarter milk and bulk milk on two large farms, implying that on farms having a high number of mastitic cows, S. aureus from infected udders may contaminate bulk milk and, subsequently, raw milk products. Sixteen (27.1%) of the S. aureus isolates tested by multiplex PCR were found to be positive for enterotoxin genes, with 15 of them carrying just one gene and one strain carrying two genes (seg and sei). The most commonly detected toxin genes were seb, sea, and sec, whereas none of our isolates possessed the see, seh, sej, or tst genes. On 75% of the dairy farms surveyed, no enterotoxigenic staphylococci were recovered from either mastitic quarter milk or bulk tank milk.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban milk Enterotoxin gene Staphylococcus aureus PFGE PCR Pulsotype
Megjelenés:	International Journal of Food Microbiology. - 118 : 2 (2007), p. 186-193. -
További szerzők:	Wagner, Martin Varga László Hein, Ingeborg Rieck, Petra Gutser, Klaus Keresztúri Péter (1975-) (biológus-ökológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Turcsányi Ibolya Béri Béla (1951-) (agrármérnök) Szabó András (1947-) (biológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL DOI
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