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001-es BibID:BIBFORM079294
035-os BibID:(cikkazonosító)e4198 (WoS)000423772400001 (Scopus)85041373987 (PMID)29404201 (PMCID)PMC5797449
Első szerző:Csizmár Nikolett (állattenyésztő mérnök)
Cím:Genetic analysis of the Hungarian draft horse population using partial mitochondrial DNA D-loop sequencing / Nikolett Csizmár, Sándor Mihók, András Jávor, Szilvia Kusza
Dátum:2018
ISSN:2167-8359
Megjegyzések: Background The Hungarian draft is a horse breed with a recent mixed ancestry created in the 1920s by crossing local mares with draught horses imported from France and Belgium. The interest in its conservation and characterization has increased over the last few years. The aim of this work is to contribute to the characterization of the endangered Hungarian heavy draft horse populations in order to obtain useful information to implement conservation strategies for these genetic stocks. Methods To genetically characterize the breed and to set up the basis for a conservation program, in the present study a hypervariable region of the mitochrondial DNA (D-loop) was used to assess genetic diversity in Hungarian draft horses. Two hundred and eighty five sequences obtained in our laboratory and 419 downloaded sequences available from Genbank were analyzed. Results One hundred and sixty-four haplotypes and thirty-six polymorphic sites were observed. High haplotype and nucleotide diversity values (Hd = 0.954 ± 0.004; ? = 0.028 ± 0.0004) were identified in Hungarian population, although they were higher within than among the different populations (Hd = 0.972 ± 0.002; ? = 0.03097 ± 0.002). Fourteen of the previously observed seventeen haplogroups were detected. Discussion Our samples showed a large intra- and interbreed variation. There was no clear clustering on the median joining network figure. The overall information collected in this work led us to consider that the genetic scenario observed for Hungarian draft breed is more likely the result of contributions from ?ancestrally' different genetic backgrounds. This study could contribute to the development of a breeding plan for Hungarian draft horses and help to formulate a genetic conservation plan, avoiding inbreeding while.
Tárgyszavak:Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Equus caballus
Genetic diversity
mtDNA
D-loop region
Hungarian draft horse
Megjelenés:PeerJ. - 6 (2018), p. 1-17. -
További szerzők:Mihók Sándor (1950-) (agrármérnök) Jávor András (1952-) (agrármérnök) Kusza Szilvia (1979-) (agrármérnök)
Pályázati támogatás:EFOP-3.6.3-VEKOP-16-2017-00008
EFOP
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2.

001-es BibID:BIBFORM079291
035-os BibID:(cikkazonosító)e4416 (WoS)000426518200008 (Scopus)85042415707 (PMID)29492347 (PMCID)PMC5827040
Első szerző:Kusza Szilvia (agrármérnök)
Cím:Kompetitive Allele Specific PCR (KASP TM) genotyping of 48 polymorphisms at different caprine loci in French Alpine and Saanen goat breeds and their association with milk composition / Szilvia Kusza, Ludovic Toma Cziszter, Daniela Elena Ilie, Maria Sauer, Ioan Padeanu, Dinu Gavojdian
Dátum:2018
ISSN:2167-8359
Megjegyzések:Using a novel and fast genotyping method called Kompetitive Allele Specific PCR (KASP TM), we carried out a pilot study on 48 single nucleotide polymorphisms (SNPs) belonging to 40 genes in French Alpine (n = 24) and Saanen (n = 25) goats reared in Romania. Furthermore, the associations of the 13 polymorphic genetic variants with milk production and composition were investigated. Thirty-five SNPs did not show polymorphism in the studied populations. Polymorphic SNPs were detected in the following genes: CAST, CLEC4E, DES, GHRHR, HSP90AA1, IL15RA, IL1RN, IL8, MITF, PPRC1, SOCS3, TNF and TNFSF13. The studied Alpine population was in Hardy-Weinberg disequilibrium at the g.62894878A>G locus (rs671391101) (P < 0.05). The results showed that four SNPs rs671391101 (GHRHR), rs640582069 (IL1RN) rs635583012 (SOCS3) and rs635969404 (IL15RA) out of the 13 polymorphic markers were significantly associated with milk production, protein, fat and lactose content in the Alpine breed. However, no significant effect was recorded in the Saanen population regarding milk yield or milk chemical composition. The current results provide new insights for the development of SNP marker-assisted selection technology in the goat industry and confirm the potential of using SNPs for the GHRHR, IL1RN, SOCS3, and IL15RA genes as candidate genes for selection, highlighting the direct implications of such genes for farm production outputs. The results from this study are relevant for future goat genomic studies and the inclusion of the associated traits into up-to-date selection schemes.
Tárgyszavak:Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
KASP
Saanen
Polymorphism
Romania
French alpine
Milk traits
Associations
Megjelenés:PeerJ. - 6 (2018), p. 1-15. -
További szerzők:Cziszter, Ludovic Toma Ilie, Daniela Elena Sauer, Maria Padeanu, Ioan Gavojdian, Dinu
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Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM077884
035-os BibID:(WoS)000462266600003 (Scopus)85063564374
Első szerző:Simon Ádám (molekuláris biológus)
Cím:Proteomics alterations in chicken jejunum caused by 24 h fasting / Simon Ádám, Gulyás Gabriella, Mészár Zoltán, Bhide Mangesh, Oláh János, Bai Péter, Csősz Éva, Jávor András, Komlósi István, Remenyik Judit, Czeglédi Levente
Dátum:2019
ISSN:2167-8359
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
chicken
proteomics
Megjelenés:PeerJ. - 7 (2019), p. e6588-1-19. -
További szerzők:Gulyás Gabriella (1985-) (agrár) Mészár Zoltán Mihály (1977-) (agrármérnök) Bhide, Mangesh Oláh János (1976-) (állattenyésztés) Bai Péter (1976-) (biokémikus) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Jávor András (1952-) (agrármérnök) Komlósi István (1960-) (agrármérnök) Gálné Remenyik Judit (1965-) (kémia tanár, okleveles vegyész) Czeglédi Levente (1977-) (agrármérnök)
Pályázati támogatás:NKFIH (K123975)
NKFIH
EFOP-3.6.2-16-2017-00001
EFOP
EFOP-3.6.3-VEKOP-16-2017-00008
EFOP
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Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM069247
035-os BibID:(WoS)000376488300001 (Scopus)84975029746
Első szerző:Sziszkosz Nikolett (molekuláris biológus)
Cím:Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers / Sziszkosz Nikolett, Mihók Sándor, Jávor András, Kusza Szilvia
Dátum:2016
Megjegyzések:The Gidran is a native Hungarian horse breed that has approached extinction several times. Phylogenetic analysis of two mitochondrial markers (D-loop and cytochrome-b) was performed to determine the genetic characterization of the Gidran for the first time as well as to detect errors in the management of the Gidran stud book. Sequencing of 686 bp of CYTB and 202 bp of the D-loop in 260 mares revealed 24 and 32 haplotypes, respectively, among 31 mare families. BLAST analysis revealed six novel CYTB and four D-loop haplotypes that have not been previously reported. The Gidran mares showed high haplotype (CYTB: 0.8735 - 0.011; D-loop: 0.9136 - 0.008) and moderate nucleotide (CYTB: 0.00472 - 0.00017; D-loop: 0.02091 - 0.00068) diversity. Of the 31 Gidran mare families, only 15CYTB (48.4%) and 17 D-loop (54.8%) distinct haplotypes were formed using the two markers separately. Merged markers created 24 (77.4%) mare families, which were in agreement with the mare families in the stud book. Our key finding was that the Gidran breed still possesses high genetic diversity despite its history. The obtained haplotypes are mostly consistent with known mare families, particularlywhen the two mtDNA markers were merged. Our results could facilitate conservation efforts for preserving the genetic diversity of the Gidran.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
CYTB, Diversity
D-loop
Gidran
Stud book error
Megjelenés:PeerJ. - 4 (2016), p. 1-15. -
További szerzők:Mihók Sándor (1950-) (agrármérnök) Jávor András (1952-) (agrármérnök) Kusza Szilvia (1979-) (agrármérnök)
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Intézményi repozitóriumban (DEA) tárolt változat
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