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001-es BibID:BIBFORM104367
035-os BibID:(WOS)000624483300001 (Scopus)85101959071
Első szerző:Ellouze, Mariem
Cím:Modeling Bacillus cereus Growth and Cereulide Formation in Cereal-, Dairy-, Meat-, Vegetable-Based Food and Culture Medium / Mariem Ellouze, Nathália Buss Da Silva, Katia Rouzeau-Szynalski, Laura Coisne, Frédérique Cantergiani, József Baranyi
Dátum:2021
ISSN:1664-302X
Megjegyzések:This study describes the simultaneous Bacillus cereus growth and cereulide formation, in culture medium and cereal-, dairy-, meat-, and vegetable-based food matrices. First, bacterial growth experiments were carried out under a wide range of temperatures (from 9 to 45 C), using the emetic reference strain F4810/72, in the above-mentioned matrices. Then, the generated data were put in a modeling framework where the response variable was a vector of two components: the concentration of B. cereus and that of its toxin, cereulide. Both were considered time-, temperature- and matrixdependent. The modeling was carried out in a series of steps: the parameters fitted in one step became the response variable of the following step. Using the square root link function, the maximum specific growth rate of the organism and the time to the appearance of quantifiable cereulide were modeled against temperature by cardinal parameters models (CPM), for each matrix. Finally, a validation study was carried out on an independent data set obtained in the same matrices and using various Bacillus cereus strains. Results showed that both growth and toxin-formation depended on the food matrix and on the environment but not in the same way. Thus, the matrix (culture medium), where the highest growth rate of B. cereus was observed, was not the medium where the shortest time to quantifiable cereulide occurred. While the cerealbased matrix generated the smallest growth rates (0.41-times smaller than culture medium did), quantifiable cereulide appeared in it at earlier times compared to the other tested matrices. In fact, three groups of matrices could be distinguished based on their ability to support cereulide formation (1) the cereal-based matrix (highest), (2) the culture medium and the dairy-based matrix (intermediate), and (3) the meat- and vegetablebased matrices (lowest). This ranking between the matrices is quite different from that based on their suitability to the growth of the organism. Our models can be used in HACCP studies, to improve shelf-life predictions and, generally, microbiological food safety assessments of products for which B. cereus is the main concern.
Tárgyszavak:Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Microbiology. - 12 (2021), p. 1-13. -
További szerzők:Buss da Silva, Nathália Rouzeau-Szynalski, Katia Coisne, Laura Cantergiani, Frédérique Baranyi József (1956-) (biomatematikus)
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2.

001-es BibID:BIBFORM104359
035-os BibID:(WOS)000704698300001 (Scopus)85116585425 (cikkazonosító)755736
Első szerző:Ellouze, Mariem
Cím:Corrigendum: Modeling Bacillus cereus Growth and Cereulide Formation in Cereal-, Dairy-, Meat-, Vegetable-Based Food and Culture Medium / Mariem Ellouze, Nathália Buss Da Silva, Katia Rouzeau-Szynalski, Laura Coisne, Frédérique Cantergiani, József Baranyi
Dátum:2021
ISSN:1664-302X
Tárgyszavak:Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény hazai lapban
folyóiratcikk
Bacillus cereus
growth
cereulide formation
predictive microbiology
food
culture medium
Megjelenés:Frontiers in Microbiology. - 12 (2021), p. 1. -
További szerzők:Buss da Silva, Nathália Rouzeau-Szynalski, Katia Coisne, Laura Cantergiani, Frédérique Baranyi József (1956-) (biomatematikus)
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3.

001-es BibID:BIBFORM103776
035-os BibID:(cikkazonosító)1000688 (WOS)000871611300001 (Scopus)85138178796
Első szerző:Farkas Zsuzsa
Cím:Probabilistic modeling and risk characterization of the chronic aflatoxin M1 exposure of Hungarian consumers / Zsuzsa Farkas, Kata Kerekes, Árpád Ambrus, Miklós Süth, Ferenc Peles, Tünde Pusztahelyi, István Pócsi, Attila Nagy, Péter Sipos, Gabriella Miklós, Anna Lőrincz10, Szilveszter Csorba, Ákos Bernard Jóźwiak
Dátum:2022
ISSN:1664-302X
Megjegyzések:Aflatoxin contamination can appear in various points of the food chain. If animals are fed with contaminated feed, AFB1 is transformed?among others?to aflatoxin M1 (AFM1) metabolite. AFM1 is less toxic than AFB1, but it is still genotoxic and carcinogenic and it is present in raw and processed milk and all kinds of milk products. In this article, the chronic exposure estimation and risk characterization of Hungarian consumers are presented, based on the AFM1 contamination of milk and dairy products, and calculated with a probabilistic method, the two-dimensional Monte-Carlo model. The calculations were performed using the R plugin (mc2d package) integrated into the KNIME (Konstanz Information Miner) software. The imulations were performed using data from the 2018?2020 food consumption survey. The AFM1 analytical data were derived from the Hungarian monitoring survey and 1,985 milk samples were analyzed within the framework of the joint project of the University of Debrecen and the National Food Chain Safety Office of Hungary (NÉBIH). Limited AFM1 concentrations were available for processed dairy products; therefore, a database of AFM1 processing factors for sour milk products and various cheeses was produced based on the latest literature data, and consumer exposure was calculated with the milk equivalent of the consumed quantities of these products. For risk characterization, the calculation of hazard index (HI), Margin of Exposure, and the hepatocellular carcinoma incidence were used. The results indicate that the group of toddlers that consume a large amount of milk and milk products are exposed to a certain level of health risk. The mean estimated daily intake of toddlers is in the range of 0.008?0.221ngkg?1 bw day?1; the 97.5th percentile exposure of toddlers is between 0.013ngkg?1 bw day?1 and 0.379ngkg?1 bw day?1, resulting in a HI above 1. According to our study, the exposure of older age groups does not pose an emergent health risk. Nevertheless, the presence of carcinogenic compounds should be kept to a minimum in the whole population.
Tárgyszavak:Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
AFM1
mycotoxin exposure assessment
probabilistic method
Megjelenés:Frontiers in Microbiology. - 13 (2022), p. 1-12. -
További szerzők:Kerekes Kata Ambrus Árpád Süth Miklós Peles Ferenc (1979-) (mikrobiológia, élelmiszer-mikrobiológia, minőségügy) Pusztahelyi Tünde (1969-) (biológus, angol-magyar szakfordító) Pócsi István (1961-) (vegyész) Nagy Attila Sipos Péter (1975-) (agrármérnök) Miklós Gabriella Lőrincz Anna Csorba Szilveszter Jóźwiak Ákos
Pályázati támogatás:2018-1.2.1-NKP-2018-00002
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4.

001-es BibID:BIBFORM082608
035-os BibID:(cikkazonosító)2861 (WOS)000504275200001 (Scopus)85077285551
Első szerző:Peles Ferenc (mikrobiológia, élelmiszer-mikrobiológia, minőségügy)
Cím:Adverse Effects, Transformation and Channeling of Aflatoxins Into Food Raw Materials in Livestock / Ferenc Peles, Péter Sipos, Zoltán Győri, Walter P. Pfliegler, Federica Giacometti, Andrea Serraino, Giampiero Pagliuca, Teresa Gazzotti, István Pócsi
Dátum:2019
ISSN:1664-302X
Megjegyzések:Aflatoxins are wide-spread harmful carcinogenic secondary metabolites produced by Aspergillus species, which cause serious feed and food contaminations and affect farm animals deleteriously with acute or chronic manifestations of mycotoxicoses. On farm, both pre-harvest and post-harvest strategies are applied to minimize the risk of aflatoxin contaminations in feeds. The great economic losses attributable to mycotoxin contaminations have initiated a plethora of research projects to develop new, effective technologies to prevent the highly toxic effects of these secondary metabolites on domestic animals and also to block the carry-over of these mycotoxins to humans through the food chain. Among other areas, this review summarizes the latest findings on the effects of silage production technologies and silage microbiota on aflatoxins, and it also discusses the current applications of probiotic organisms and microbial products in feeding technologies. After ingesting contaminated foodstuffs, aflatoxins are metabolized and biotransformed differently in various animals depending on their inherent and acquired physiological properties. These mycotoxins may cause primary aflatoxicoses with versatile, species-specific adverse effects, which are also dependent on the susceptibility of individual animals within a species, and will be a function of the dose and duration of aflatoxin exposures. The transfer of these undesired compounds from contaminated feed into food of animal origin and the aflatoxin residues present in foods become an additional risk to human health, leading to secondary aflatoxicoses. Considering the biological transformation of aflatoxins in livestock, this review summarizes (i) the metabolism of aflatoxins in different animal species, (ii) the deleterious effects of the mycotoxins and their derivatives on the animals, and (iii) the major risks to animal health in terms of the symptoms and consequences of acute or chronic aflatoxicoses, animal welfare and productivity. Furthermore, we traced the transformation and channeling of Aspergillus-derived mycotoxins into food raw materials, particularly in the case of aflatoxin contaminated milk, which represents the major route of human exposure among animal-derived foods. The early and reliable detection of aflatoxins in feed, forage and primary commodities is an increasingly important issue and, therefore, the newly developed, easy-to-use qualitative and quantitative aflatoxin analytical methods are also summarized in the review.
Tárgyszavak:Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Microbiology. - 10 (2019), p. 1-26. -
További szerzők:Sipos Péter (1975-) (agrármérnök) Győri Zoltán (1948-) (vegyész) Pfliegler Valter Péter (1986-) (molekuláris biológus) Giacometti, Federica Serraino, Andrea Pagliuca, Giampiero Gazzotti, Teresa Pócsi István (1961-) (vegyész)
Pályázati támogatás:Debreceni Egyetem Biotechnológia tématerületi program
FIKP
2018-1.2.1-NKP-2018-00002
Egyéb
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5.

001-es BibID:BIBFORM082438
035-os BibID:(cikkazonosító)2921 (WOS)000517458200001 (Scopus)85083912366
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:The Aspergilli and their mycotoxins : metabolic interactions with plants and the soil biota / Valter P. Pfliegler, István Pócsi, Zoltán Győri, Tünde Pusztahelyi
Dátum:2020
ISSN:1664-302X
Megjegyzések:Species of the highly diverse fungal genus Aspergillus are well-known agricultural pests and, most importantly, producers of various mycotoxins threatening food safety worldwide. Mycotoxins are studied predominantly from the perspectives of human and livestock health. Meanwhile, their roles are far less known in nature. However, to understand the factors behind mycotoxin production, the roles of the toxins of the Aspergilli must be understood from a complex ecological perspective, taking mold-plant, mold-microbe, and mold-animal interactions into account. The Aspergilli may switch between saprophytic and pathogenic lifestyles, and the production of secondary metabolites, such as mycotoxins, may vary according to these fungal ways of life. Recent studies highlighted the complex ecological network of soil microbiotas determining the niches that Aspergilli can fill in. Interactions with the soil microbiota and soil macro-organisms determine the role of secondary metabolite production to a great extent. While, upon infection of plants, metabolic communication including fungal secondary metabolites like aflatoxins, gliotoxin, patulin, cyclopiazonic acid, ochratoxin, influences the fate of both the invader and the host. In this review, the role of mycotoxin producing Aspergillus species and their interactions in the ecosystem are discussed. We intend to highlight the complexity of the roles of the main toxic secondary metabolites as well as their fate in natural environments and agriculture, a field that still has important knowledge gaps.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Microbiology. - 10 (2020), p. 1-45. -
További szerzők:Pócsi István (1961-) (vegyész) Győri Zoltán (1948-) (vegyész) Pusztahelyi Tünde (1969-) (biológus, angol-magyar szakfordító)
Pályázati támogatás:NKFIH 2018-1.2.1-NKP
Egyéb
OTKA K119494
OTKA
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM107477
035-os BibID:(cikkazonosító)901484 (WOS)000886959400001 (Scopus)85134918411
Első szerző:Rukit, Junyaluck
Cím:Roles of alternative sigma factors in invasion and growth characteristics of Listeria monocytogenes 10403S into human epithelial colorectal adenocarcinoma Caco-2 cell / Junyaluck Rukit, Atsadang Boonmee, Teeratas Kijpornyongpan, Kan Tulsook, József Baranyi, Soraya Chaturongakul
Dátum:2022
ISSN:1664-302X
Megjegyzések:Listeria monocytogenes is a Gram-positive facultative intracellular bacterium with a broad host range. With its housekeeping sigma factor and four alternative ones (namely SigB, SigC, SigH, and SigL), L. monocytogenes can express genes in response to changing environments. However, the roles of these sigma factors in intracellular survival are still unclear. The objectives of this study were to characterize the role of each alternative sigma factor on L. monocytogenes invasion and growth inside human epithelial colorectal adenocarcinoma Caco-2 cells. We used L. monocytogenes 10403S wild type and its 15 alternative sigma factor deletion mutants at a multiplicity of infection of 100 and 1 in invasion and intracellular growth assays in the Caco-2 cells, respectively. At 1.5, 2, 4, 6, 8, 10, and 12 h post-infection, Caco-2 cells were lysed, and intracellular L. monocytogenes were enumerated on brain-heart infusion agar. Colony-forming and growth rates were compared among strains. The results from phenotypic characterization confirmed that (i) SigB is the key factor for L. monocytogenes invasion and (ii) having only SigA (Delta sigBCHL strain) is sufficient to invade and multiply in the host cell at similar levels as the wild type. Our previous study suggested the negative role of SigL in bile stress response. In this study, we have shown that additional deletion of the rpoN (or sigL) gene to Delta sigB, Delta sigC, or Delta sigH could restore the impaired invasion efficiencies of the single mutant, suggesting the absence of SigL could enhance host invasion. Therefore, we further investigated the role of SigL during extracellular and intracellular life cycles. Using RNA sequencing, we identified 118 and 16 SigL-dependent genes during the extracellular and intracellular life cycles, respectively. The sigL gene itself was induced by fivefolds prior to the invasion, and 5.3 folds during Caco-2 infection, further suggesting the role of SigL in intracellular growth.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Listeria monocytogenes
invasion
transcriptomics
RNA sequencing
Caco-2 cell
Megjelenés:Frontiers in Microbiology. - 13 (2022), p. 1-13. -
További szerzők:Boonmee, Atsadang Kijpornyongpan, Teeratas Tulsook, Kan Baranyi József (1956-) (biomatematikus) Chaturongakul, Soraya
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