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001-es BibID:	BIBFORM119607
035-os BibID:	(WoS)001180768600001 (Scopus)85187168868
Első szerző:	Brysch-Herzberg, Michael
Cím:	Reinstatement of the fission yeast species Schizosaccharomyces versatilis Wickerham et Duprat, a sibling species of Schizosaccharomyces japonicus / Michael Brysch-Herzberg, Guo-Song Jia, Matthias Sipiczki, Martin Seidel, Wen-Cai Zhang, Li-Lin Du
Dátum:	2024
ISSN:	0749-503X
Megjegyzések:	Schizosaccharomyces japonicus Yukawa et Maki (1931) and Schizosaccharomyces versatilis Wickerham et Duprat (1945) have been treated as varieties of S. japonicus or as conspecific, based on various approaches including mating trials and nDNA/nDNA optical reassociation studies. However, the type strains of S. japonicus and S. versatilis differ by five substitutions (99.15% identity) and one 1-bp indel in the sequences of the D1/D2 domain of the 26S rRNA gene, and 23 substitutions (96.3% identity) and 31-bp indels in the sequences of internal transcribed spacer (ITS) of rRNA, suggesting that they may not be conspecific. To reassess their taxonomic status, we conducted mating trials and whole-genome analyses. Mating trials using the type strains showed a strong but incomplete prezygotic sterility barrier, yielding interspecies mating products at two orders of magnitude lower efficiency than intraspecies matings. These mating products, which were exclusively allodiploid hybrids, were unable to undergo the haplontic life cycle of the parents. We generated chromosome-level gap-less genome assemblies for both type strains. Whole genome sequences yielded an average nucleotide identity (ANI) of 86.4%, indicating clear separation of S. japonicus and S. versatilis. Based on these findings, we propose the reinstatement of S. versatilis as a distinct species (holotype strain: CBS 103T and ex-types: NRRL Y-1026, NBRC 1607, ATCC 9987, PYCC 7100; Mycobank no.: 847838).
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk genomic rearrangements reproductive incompatibility Schizosaccharomyces versatilis species delimitation
Megjelenés:	Yeast. - 41 : 3 (2024), p. 108-127. -
További szerzők:	Jia, Guo-Song Sipiczki Mátyás (1948-) (biológus) Seidel, Martin Zhang, Wen-Cai Du, Li-Lin
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM113641
035-os BibID:	(Scopus)85160928751 (Wos)000994022100001
Első szerző:	Brysch-Herzberg, Michael
Cím:	Schizosaccharomyces lindneri sp. nov., a fission yeast occurring in honey / Michael Brysch-Herzberg, Guo-Song Jia, Matthias Sipiczki, Martin Seidel, Wen Li, Imen Assali, Li-Lin Du
Dátum:	2023
ISSN:	0749-503X
Megjegyzések:	Two strains of fission yeast were isolated from honey. They differ from the type strain of Schizosaccharomyces octosporus by three substitutions in the D1/D2 domain of the nuclear 26S large subunit ribosomal RNA (rRNA) gene sequence, resulting in a 99.5% identity. In the internal transcribed spacer (ITS) region (consisting of ITS1, 5.8S rDNA, and ITS2), the strains differ from S. octosporus by 16 gaps and 91 substitutions, which is equivalent to an identity of 88.1%. Genome sequencing on one of the new strains revealed that the average nucleotide identity (ANI) between its genome and the reference genome of S. octosporus is 90.43% and there exist major genome rearrangements between the two genomes. Mating analysis revealed that S. octosporus and one of the new strains are completely reproductively separated. A strong prezygotic barrier exists and the few mating products consist of diploid hybrids that do not form recombinant ascospores. In the new strains, asci are either zygotic, arising from conjugation, or they develop without conjugation from asexual cells (azygotic). Compared to the currently recognized Schizosaccharomyces species, the spectrum of nutrients that are assimilated by the new strains is restricted. Of the 43 carbohydrates that were included in the physiological standard tests, only 7 were assimilated. According to the results of the genome sequence analysis, the mating trials, and the phenotypic characterization, the new species Schizosaccharomyces lindneri is described to accommodate the two strains (holotype: CBS 18203T and ex-type: MUCL 58363; MycoBank no.: MB 847838).
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk genomic rearrangements hybridization Schizosaccharomyces lindneri taxogenomics
Megjelenés:	Yeast. - 40 : 7 (2023), p. 237-253. -
További szerzők:	Jia, Guo-Song Sipiczki Mátyás (1948-) (biológus) Seidel, Martin Li, Wen Assali, Imen Du, Li-Lin
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM093483
035-os BibID:	(Scopus)85104748956 (WOS)000647236400001
Első szerző:	Rácz Hanna Viktória (biológus)
Cím:	How to characterize a strain? Clonal heterogeneity in industrial influences both phenotypes and heterogeneity in phenotypes / Hanna Viktória Rácz, Fezan Mukhtar, Alexandra Imre, Zoltán Rádai, Andreas Károly Gombert, Tamás Rátonyi, János Nagy, István Pócsi, Walter P. Pfliegler
Dátum:	2021
ISSN:	0749-503X
Megjegyzések:	Populations of microbes are constantly evolving heterogeneity that selection acts upon, yet heterogeneity is non?trivial to assess methodologically. The necessary practice of isolating single cell colonies and thus, subclone lineages for establishing, transferring, and using a strain results in single?cell bottlenecks with a generally neglected effect on the characteristics of the strain itself. Here, we present evidence that various subclone lineages for industrial yeasts sequenced for recent genomic studies show considerable differences, ranging from loss?of?heterozygosity to aneuploidies. Subsequently, we assessed whether phenotypic heterogeneity is also observable in industrial yeast, by individually testing subclone lineages obtained from products. Phenotyping of industrial yeast samples and their newly isolated subclones showed that single?cell bottlenecks during isolation can indeed considerably influence the observable phenotype. Next, we decoupled fitness distributions on the level of individual cells from clonal interference by plating single cell colonies and quantifying colony area distributions. We describe and apply an approach using statistical modeling to compare the heterogeneity in phenotypes accross samples and subclone lineages. One strain was further used to show how individual subclonal lineages are remarkably different not just in phenotype, but also in the level of heterogeneity in phenotype. With these observations, we call attention to the fact that choosing an initial clonal lineage from an industrial yeast strain may vastly influence downstream performances and observations on karyotype, phenotype, and also on heterogeneity.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk aneuploidy domestication Fermented foods and beverages genomic structural variation phenotypic variability Saccharomyces boulardii
Megjelenés:	Yeast. - 38 : 8 (2021), p. 453-470. -
További szerzők:	Mukhtar, Fezan Imre Alexandra (1992-) (biomérnök, biotechnológus) Rádai Zoltán (1991-) (biológus) Gombert Andreas Károly Rátonyi Tamás (1967-) (agrármérnök) Nagy János (1951-) (agrármérnök, mérnök-tanár) Pócsi István (1961-) (vegyész) Pfliegler Valter Péter (1986-) (molekuláris biológus)
Pályázati támogatás:	GINOP-2.2.1-15-2016-00001 GINOP NKFIH-1150-6/2019 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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