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001-es BibID:BIBFORM102892
035-os BibID:(cikkazonosító)677 (WoS)000846075500001 (Scopus)85137460294
Első szerző:Abdalla, Neama
Cím:An Academic and Technical Overview on Plant Micropropagation Challenges / Neama Abdalla, Hassan El-Ramady, Mayada K. Seliem, Mohammed E. El-Mahrouk, Naglaa Taha, Yousry Bayoumi, Tarek A. Shalaby, Judit Dobránszki
Dátum:2022
ISSN:2311-7524
Megjegyzések:The production of micropropagated plants in plant-tissue-culture laboratories and nurseries is the most important method for propagation of many economic plants. Micropropagation based on tissue-culture technology involves large-scale propagation, as it allows multiplication of a huge number of true-to-type propagules in a very short time and in a very limited space, as well as all year round, regardless of the climate. However, applying plant-tissue-culture techniques for the commercial propagation of plants may face a lot of obstacles or troubles that could result from technical, biological, physiological, and/or genetical reasons, or due to overproduction or the lack of facilities and professional technicians, as shown in the current study. Moreover, several disorders and abnormalities are discussed in the present review. This study aims to show the most serious problems and obstacles of plant micropropagation, and their solutions from both scientific and technical sides. This review, as a first report, includes different challenges in plant micropropagation (i.e., contamination, delay of subculture, burned plantlets, browning, in vitro rooting difficulty, somaclonal variations, hyperhydricity, shoot tip necrosis, albino plantlets, recalcitrance, shoot abnormalities, in vitro habituation) in one paper. Most of these problems are related to scientific and/or technical reasons, and they could be avoided by following the micropropagation protocol suitable for each plant species. The others are dominant in plant-tissue-culture laboratories, in which facilities are often incomplete, or due to poor infrastructure and scarce funds.
Tárgyszavak:Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
acclimatization
browning
contamination
delay of subculture
hyperhydricity
recalcitrance
somaclonal variations
totipotency
Megjelenés:Horticulturae. - 8 : 8 (2022), p. 1-28. -
További szerzők:El-Ramady, Hassan (1969-) (agrármérnök) Seliem, Mayada Kadri Elmahrouk, Mohammed Taha, Naglaa Bayoumi, Yousry Shalaby, Tarek Dobránszki Judit (1967-) (biológus)
Pályázati támogatás:TKP2021-EGA-20
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001-es BibID:BIBFORM102634
035-os BibID:(cikkazonosító)591 (WoS)000833676900001 (Scopus)85133533530
Első szerző:Hanász Alexandra (környezetgazdálkodási agrármérnök)
Cím:Responses of Potato (Solanum tuberosum L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot Culture / Hanász Alexandra, Dobránszki Judit, Mendler-Drienyovszki Nóra, Zsombik László, Magyar-Tábori Katalin
Dátum:2022
ISSN:2311-7524
Megjegyzések:In vitro experiments were conducted to study the responses of potato (Solanum tuberosum L.) genotypes to osmotic stress. In vitro shoot cultures of 27 breeding lines and their drought-tolerant parents (referent lines: C103 and C107) were tested under osmotic stress induced by addition of PEG 6000 (Mw = 6000; 5.0, 7.5, 10.0%, w/v), D-mannitol (0.1, 0.2, 0.3 M) and PEG 600 (Mw = 600; 2.5, 5.0, 7.5%, w/v) to the Murashige-Skoog medium. Stress index (SI) was calculated from shoot length (SL) and root length (RL), root numbers (RN) and the rate of surviving shoots (SR) (SISL;RL;RN;SR = Parameter(SL;RL;RN;SR) of treated shoots/Parameter(SL;RL;RN;SR) of control shoots x 100) to compare genotypes. In the average of each breeding line and concentration, the osmotic agents resulted in SI values of 40.1, 60.8, 82.6 and 76.0 for SISL, SIRL, SIRN and SISR, respectively. In general, all SI values of C103 and SIRL,RN of C107 were significantly higher than those of the breeding lines. Nine breeding lines were found to be promising based on their final ranking. According to the results, 7.5% and 10% PEG 6000 or 0.2 M and 0.3 M D-mannitol treatments proved to be suitable for the selection of osmotic stress-tolerant genotypes.
Tárgyszavak:Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
polyethylene glycol
D-mannitol
tissue culture
stress index
morphological traits
Megjelenés:Horticulturae. - 8 : 7 (2022), p. 1-24. -
További szerzők:Dobránszki Judit (1967-) (biológus) Mendlerné Drienyovszki Nóra (1977-) (agrármérnök) Zsombik László (1976-) (agrármérnök) Magyarné Tábori Katalin (1965-) (kertészmérnök)
Pályázati támogatás:TKP2021- EGA-20
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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