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1.

001-es BibID:BIBFORM004699
Első szerző:Bacsó Zsolt (biofizikus)
Cím:INF-gamma rearranges membrane topography of MHC-I and ICAM-1 in colon carcinoma cells / Bacso, Z., Bene, L., Damjanovich, L., Damjanovich, S.
Dátum:2002
Megjegyzések:Flow-cytometric fluorescence energy transfer (FCET) measurements between fluorescently labeled cell surface MHC-I and ICAM-1 molecules indicated similar receptor patterns in the plasma membrane of interferon-gamma (INF-gamma)-treated colon carcinoma cells as those observed earlier at the surface of lymphoid cells. INF-gamma activation significantly increased the density of MHC-I and ICAM-1 proteins in the membrane. This increase in receptor density was accompanied by decreased proximity level of the homo-associated MHC-I receptors. Hetero-association of MHC-I and ICAM-1 molecules was increased by INF-gamma treatment. INF-gamma changed neither hetero- nor homo-association of transferrin receptors. By staining the sphingomyelin/cholesterol-enriched lipid microdomains with fluorescently labeled cholera toxin B subunit, we found an increase in the amount of lipid-raft associated G(M1)-gangliosides due to INF-gamma treatment. Confocal microscopic results and FCET measurements show that MHC-I and ICAM-1 are components of G(M1)-ganglioside containing lipid-rafts and also support an increase in the size of these lipid-rafts upon INF-gamma treatment.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Carcinoma
Cell Membrane
chemistry
Cholera Toxin
Colonic Neoplasms
drug effects
Energy Transfer
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescence
G(M1) Ganglioside
Histocompatibility Antigens Class I
Human
Hungary
Intercellular Adhesion Molecule-1
Interferon Type II
Membrane Microdomains
metabolism
Microscopy,Confocal
pathology
pharmacology
Protein Binding
Receptors,Cell Surface
Receptors,Transferrin
Support,Non-U.S.Gov't
Tumor Cells,Cultured
Megjelenés:Biochemical and Biophysical Research Communications. - 290 : 2 (2002), p. 635-640. -
További szerzők:Bene László (1963-) (biofizikus) Damjanovich László (1960-) (általános sebész) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:DOI
elektronikus változat
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2.

001-es BibID:BIBFORM004830
035-os BibID:WOS:000223675800046
Első szerző:Bene László (biofizikus)
Cím:Membrane topography of HLA I, HLA II, and ICAM-1 is affected by IFN-gamma in lipid rafts of uveal melanomas / László Bene, Andrea Bodnár, Sándor Damjanovich, György Vámosi, Zsolt Bacsó, János Aradi, András Berta, Judit Damjanovich
Dátum:2004
Megjegyzések:The lateral distribution and colocalization of HLA I, HLA-DR, and ICAM-1 proteins was studied for the first time in the plasma membrane of two human uveal melanoma cell lines, OCM-1 and OCM-3. Our fluorescence resonance energy transfer and confocal laser scanning microscopic experiments revealed that these molecules are mostly confined to the same membrane regions, where they form similar protein patterns (homo- and hetero-associates) to those found previously on other cell types of lymphoid as well as colorectal carcinoma origin. Confocal microscopic colocalization experiments with GM(1) gangliosides and the GPI-anchored CD59 molecules showed enrichment of HLA I, HLA-DR, and ICAM-1 molecules in specific membrane domains (lipid rafts) excluding the transferrin receptor. IFN-gamma remarkably increased the expression levels of these molecules and rearranged their association patterns, which can affect the adoptive immune response of effector cells
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antigens
Biophysics
Carcinoma
Cell Line
Cells
Energy Transfer
Flow Cytometry
Fluorescence
Fluorescence Resonance Energy Transfer
Histocompatibility Antigens
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Human
Humans
Hungary
Intercellular Adhesion Molecule-1
Interferon Type II
Melanoma
Membrane Microdomains
metabolism
Microscopy,Confocal
Proteins
Research
Support
Uveal Neoplasms
egyetemen (Magyarországon) készült közlemény
Megjelenés:Biochemical and Biophysical Research Communications. - 322 : 2 (2004), p. 678-683. -
További szerzők:Dóczy-Bodnár Andrea (1970-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Vámosi György (1967-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Aradi János (1942-) (biokémikus, vegyész) Berta András (1955-) (szemész, gyermekszemész) Damjanovich Judit (1963-) (szemész)
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM001204
Első szerző:Bene László (biofizikus)
Cím:Colorectal carcinoma rearranges cell surface protein topology and density in CD4+ T cells / Bene L., Kanyári Z., Bodnár A., Kappelmayer J., Waldmann T. A., Vámosi G., Damjanovich L.
Dátum:2007
Megjegyzések:Previously, we described conserved protein clusters includingMHCI and II glycoproteins, ICAM-1 adhesion molecules, and interleukin- 2 and -15 receptors in lipid rafts of several human cell types. Differential protein interactions can modulate function, thus influence cell fate. Therefore, we analyzed supramolecular clusters of CD4+ T cells from draining lymph nodes and peripheral blood of colorectal carcinoma patients, and compared these to healthy controls. Superclusters of MHC I and II with IL-2/15 receptors were identified by confocal microscopy on all cell types. Flow-cytometricFRETrevealed molecular associations of these proteins with each other and withICAM-1 as well. In draining lymph nodes expression levels of all these proteins were lower, and interactions, particularly between IL-2/15 receptors and MHC molecules weakened or disappeared as compared to the control. Stimuli/local conditions can rearrange cell surface protein patterns on the same cell type in the same patient, having important implications on further function and cell fate.
Tárgyszavak:Orvostudományok Klinikai orvostudományok Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
CD4+ T cells
colorectal carcinoma
MHC I
MHC II
IL-2R
IL-15R
ICAM-1
receptor clustering
FRET
Flow cytometry
Confocal microscopy
Membrane protein association
Draining lymph node
Megjelenés:Biochemical and Biophysical Research Communications. - 361 : 1 (2007), p. 202-207. -
További szerzők:Kanyári Zsolt (1964-) (orvos) Dóczy-Bodnár Andrea (1970-) (biofizikus) Kappelmayer János (1960-) (laboratóriumi szakorvos) Waldmann, Thomas A. Vámosi György (1967-) (biofizikus) Damjanovich László (1960-) (általános sebész)
Internet cím:elektronikus változat
DOI
Borító:

4.

001-es BibID:BIBFORM046274
Első szerző:Gáspár Rezső (biofizikus)
Cím:[Béta]-scorpion toxin 2 from Centruroides noxius blocks voltage-gated K+ channels in human lymphocytes / Gaspar R., Bene L., Damjanovich S., Munoz-Garay C., Calderon-Aranda E. S., Possani L. D.
Dátum:1995
ISSN:0006-291X
Megjegyzések:Using the patch-clamp technique, we determined that beta-scorpion toxin 2 from Centruroides noxius Hoffmann decreased whole-cell n-type K+ currents in human peripheral blood lymphocytes, with a half blocking concentration of approx. 5 microM. Toxin-2-accelerated inactivation, however, did not influence the kinetics of activation of the K+ conductance. The percentage increase in K+ channel inactivation rate and the degree of drug-induced block was independent of membrane potential. K+ channel block by Toxin 2 was instantaneous, not removable by washing with drug free extracellular solution. However, 10 mg/ml BSA in the bath lifted the toxin-induced block almost instantaneously and completely. Flow cytometric membrane potential measurements with the oxonol dye showed that Toxin 2 depolarizes human lymphocytes in concert with its K+ channel blocking effect.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications. - 213 : 2 (1995), p. 419-423. -
További szerzők:Bene László (1963-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Munoz-Garay, Carlos Calderon-Aranda, Emma S. Possani, Lourival Domingos
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

5.

001-es BibID:BIBFORM004839
035-os BibID:(WOS)000220105600025 (scopus)1342345242
Első szerző:Nagy Henrietta
Cím:Distinct groups of multidrug resistance modulating agents are distinguished by competition of P-glycoprotein-specific antibodies / Nagy, H., Goda, K., Fenyvesi, F., Bacso, Z., Szilasi, M., Kappelmayer, J., Lustyik, G., Cianfriglia, M., Szabo, G.
Dátum:2004
Megjegyzések:P-glycoprotein (Pgp) is one of the ABC transporters responsible for the multidrug resistance of cancer cells. The conformational changes of Pgp that occur in the presence of substrates/modulators or ATP depletion are accompanied by the up-shift of UIC2 monoclonal antibody (mAb) binding. In the case of cyclosporin A, vinblastine or valinomycin, this up-shift was found to be concomitant with the near-complete suppression of labeling with other mAbs specific for Pgp epitopes overlapping with UIC2, while pre-treatment with verapamil or Tween 80 brings about a modest suppression. Here we have extended these observations to 44 Pgp interacting agents, and found that only 8 fall into the cyclosporin-like category, inducing a conformational state characterized by the complete UIC2 dominance. The rest of the drugs either did not affect antibody competition or had a modest effect. Thus, Pgp substrates/modulators can be classified into distinct modalities based on the conformational change they elicit.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Adenosine
Adenosine Triphosphatases
Animals
Anti-Bacterial Agents
Antibodies
Antibodies,Monoclonal
Antineoplastic Agents
Binding,Competitive
Biophysics
Calcium
Calcium Channel Blockers
Cells
Cyclosporine
Detergents
Drug Resistance,Multiple
Drug Resistance,Neoplasm
Epitopes
Flow Cytometry
Fluorescein
Fluoresceins
genetics
Humans
Hungary
immunology
Ivermectin
metabolism
Mice
Nih 3T3 Cells
P-Glycoprotein
pharmacology
physiology
Research
Substrate Specificity
Support
Valinomycin
Verapamil
Vinblastine
egyetemen (Magyarországon) készült közlemény
Megjelenés:Biochemical and Biophysical Research Communications. - 315 : 4 (2004), p. 942-949. -
További szerzők:Goda Katalin (1969-) (biofizikus) Fenyvesi Ferenc (1977-) (gyógyszerész, gyógyszertechnológus) Bacsó Zsolt (1963-) (biofizikus) Szilasi Mária (1953-) (tüdőgyógyász, klinikai immunológus, allergológus, belgyógyász) Kappelmayer János (1960-) (laboratóriumi szakorvos) Lustyik György Cianfriglia, Maurizio Szabó Gábor (1953-) (biofizikus)
Internet cím:elektronikus változat
DOI
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6.

001-es BibID:BIBFORM005090
Első szerző:Nagy Péter (biofizikus)
Cím:ICAM-1 inhibits the homocluster formation of MHC-I in colon carcinoma cells / Nagy, P., Vamosi, G., Damjanovich, S., Damjanovich, L.
Dátum:2006
ISSN:006-291X (Print)
Megjegyzések:ICAM-1 and MHC-I proteins play fundamental roles in antigen presentation, activation of T lymphocytes, and immune responses against tumor cells. Both of them participate in the formation of lipid raft-associated membrane protein clusters. We found significant colocalization between ICAM-1 and MHC-I at the level of large-scale associations. We combined RNA interference and fluorescence resonance energy transfer studies to show that ICAM-1 promotes the partial disassembly of MHC-I homoclusters on LS-174T colon carcinoma cells. Interferon-gamma (IFN-gamma) treatment induced an increase in the expression of MHC-I and ICAM-1 resulting in decreased MHC-I homoassociation. Small interfering RNAs directed against ICAM-1 restored the homoassociation of MHC-I without influencing the expression level of MHC-I by eliminating ICAM-1 molecules interspersed in MHC-I clusters. We conclude that the composition of membrane protein clusters is dynamically altered in response to both physiological and experimentally elicited changes in antigen expression levels.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antigen Presentation
Antigens
Biophysics
Carcinoma
Cell Line, Tumor
Cells
Colonic Neoplasms
Energy Transfer
Fluorescence
Fluorescence Resonance Energy Transfer
genetics
Histocompatibility Antigens
Histocompatibility Antigens Class I
Humans
Hungary
Intercellular Adhesion Molecule-1
Lymphocytes
metabolism
Protein Binding
Proteins
Research
RNA,Small Interfering
Support
T-Lymphocytes
Megjelenés:Biochemical and Biophysical Research Communications. - 347 : 3 (2006), p. 758-763. -
További szerzők:Vámosi György (1967-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Damjanovich László (1960-) (általános sebész)
Internet cím:DOI
elektronikus változat
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7.

001-es BibID:BIBFORM004716
Első szerző:Penyige András (molekuláris genetikus)
Cím:Depolarization of the membrane potential by beta-lactams as a signal to induce autolysis / Penyige, A., Matko, J., Deak, E., Bodnar, A., Barabas, G.
Dátum:2002
Megjegyzések:The effect of beta-lactam antibiotics that are known to inhibit cell wall biosynthesis and induce cell wall autolysis on the electrophysiological state of the plasma membrane in Streptomyces griseus was studied. Addition of various beta-lactam antibiotics induced a dose- and growth-stage-dependent depolarization of the membrane potential of Streptomyces griseus. The hydrolyzed biologically inactive derivative penicilloic acid had no depolarizing effect on the membrane potential. The ionophore gramicidin D, while depolarizing the membrane potential, also induced a dose-dependent increase in cell wall lysis. These observations suggest that alteration of the transmembrane potential could be an important signal in triggering cell wall autolysis of S. griseus.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Anti-Bacterial Agents
Antibiotics
Autolysis
biosynthesis
Carbocyanines
Cefotaxime
Coloring Agents
drug effects
Drug Interactions
Gramicidin
Hungary
Ionophores
Membrane Potentials
metabolism
Penicillin G
pharmacology
Research
Streptomyces
Streptomyces griseus
Support
Megjelenés:Biochemical and Biophysical Research Communications. - 290 : 4 (2002), p. 1169-1175. -
További szerzők:Matkó János (1952-) (biológus) Deák Eleonóra Dóczy-Bodnár Andrea (1970-) (biofizikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus)
Internet cím:elektronikus változat
DOI
Borító:

8.

001-es BibID:BIBFORM004670
Első szerző:Péter Mózes (orvos, neuroradiológus) ifj.
Cím:Blockage of human T lymphocyte Kv1.3 channels by Pi1, a novel class of scorpion toxin / Peter, M., Jr., Hajdu, P., Varga, Z., Damjanovich, S., Possani, L. D., Panyi, G., Gaspar, R.
Dátum:2000
Megjegyzések:Using the patch-clamp technique we determined that Pandinus imperator toxin Pi1, a recently described peptide toxin having four disulfide bridges instead of the usual three in scorpion toxins, blocked Kv1.3 channels of human T lymphocytes from the extracellular side with a 1:1 stoichiometry. Kv1.3 block was instantaneous and removable with toxin-free extracellular solution. The toxin did not influence activation or inactivation of the channels. We found that Pi1 blocked Kv1.3 with less affinity (K(d) = 11.4 nM) than the structurally related three disulfide bridge containing toxins Pi2 (50 pM) and Pi3 (0.5 nM). The fourth disulfide bridge in Pi1 had no influence on the channel binding ability of the toxin; the less effective block was due to differences in amino acid side chain properties at positions 11 and 35.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Amino Acid Sequence
Amino Acids
Animal
chemistry
Disulfides
drug effects
Human
Hungary
Kinetics
Lymphocytes
metabolism
Molecular Sequence Data
Patch-Clamp Techniques
pharmacology
Potassium
Potassium Channels
Protein Binding
Scorpion Venoms
Scorpions
Sequence Homology,Amino Acid
Support,Non-U.S.Gov't
T-Lymphocytes
Time Factors
Toxins
Megjelenés:Biochemical and Biophysical Research Communications. - 278 : 1 (2000), p. 34-37. -
További szerzők:Hajdu Péter (1975-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító) Damjanovich Sándor (1936-2017) (biofizikus) Possani, Lourival Domingos Panyi György (1966-) (biofizikus) Gáspár Rezső (1944-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

9.

001-es BibID:BIBFORM004635
Első szerző:Péter Mózes (orvos, neuroradiológus) ifj.
Cím:Pandinus imperator scorpion venom blocks voltage-gated K+ channels in human lymphocytes / Peter, M. Jr., Varga, Z., Panyi, G., Bene, L., Damjanovich, S., Pieri, C., Possani, L. D., Gaspar, R.
Dátum:1998
ISSN:006-291X
Megjegyzések:Using the patch-clamp technique, we determined that Pandinus imperator scorpion venom blocked whole-cell n-type K+ currents in human peripheral blood lymphocytes in a dose-dependent manner with Kd = 0.02 microgram/ml. K+ channel block was instantaneous and removable by washing with venom-free extracellular solution. The venom-induced block was independent of membrane potential. The venom did not influence activation and inactivation kinetics of the K+ channels, however, accelerated recovery from inactivation. Purified peptides Pi1, Pi2, and Pi3 from the P. imperator venom powerfully blocked Kv1.3 channels in human lymphocytes with Kd values of 9.7 nM, 50 pM, and 0.5 nM, respectively. Flow cytometric membrane potential measurements with the oxonol dye showed that Pi2, the most effective peptide toxin of the P. imperator venom, depolarizes human lymphocytes in accordance with its K+ channel blocking effect.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Barbiturates
blood
drug effects
Electrophysiology
Flow Cytometry
Fluorescent Dyes
Human
isolation and purification
Isoxazoles
Kinetics
Lymphocytes
Membrane Potentials
metabolism
Patch-Clamp Techniques
pharmacology
physiology
Potassium
Potassium Channel Blockers
Potassium Channels
Protein Binding
Scorpion Venoms
Support, Non-U.S.Gov't
Support, U.S.Gov't, P.H.S.
Toxins
Megjelenés:Biochemical and Biophysical Research Communications. - 242 : 3 (1998), p. 621-625. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Panyi György (1966-) (biofizikus) Bene László (1963-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Pieri, Carlo Possani, Lourival Domingos Gáspár Rezső (1944-) (biofizikus)
Internet cím:DOI
elektronikus változat
Borító:

10.

001-es BibID:BIBFORM004637
Első szerző:Recchioni, Rina
Cím:Melatonin increases the intensity of respiratory burst and prevents L-selectin shedding in human neutrophils in vitro / Recchioni, R., Marcheselli, F., Moroni, F., Gaspar, R., Damjanovich, S., Pieri, C.
Dátum:1998
ISSN:006-291X
Megjegyzések:Effects of melatonin priming of neutrophils and subsequent increase of phorbol 12-miristate 13-acetate stimulated respiratory burst were investigated on the modulation of L-selectin shedding and MAC-1 upregulation. Respiratory burst related H2O2 production and adhesion molecule expression were quantified by flow cytometry. Phorbol 12-miristate 13-acetate dose dependence of intracellular oxidation and adhesion molecule expression showed no relationship between respiratory burst intensity and MAC-1 expression or L-selectin shedding. Treatment of cells with 12.5 nM phorbol 12-miristate 13-acetate resulted in less than 20% of the respiratory burst response, however it induced 91.7% of total MAC-1 expression and 62.8% of L-selectin shedding. Melatonin priming experiments showed also no connection between the extent of respiratory burst and MAC-1 expression, however melatonin priming almost completely prevented L-selectin down-regulation elicited by phorbol 12-miristate 13-acetate, without affecting MAC-1 expression. It is suggested that melatonin may inhibit metalloproteases responsible for L-selectin cleavage
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
biosynthesis
blood
Dose-Response Relationship,Drug
drug effects
Flow Cytometry
Human
Hydrogen Peroxide
In Vitro
Kinetics
L-Selectin
Macrophage-1 Antigen
Melatonin
Neutrophils
pharmacology
physiology
Research
Respiratory Burst
Rhodamine 123
Support, Non-U.S.Gov't
Tetradecanoylphorbol Acetate
Megjelenés:Biochemical and Biophysical Research Communications. - 252 : 1 (1998), p. 20-24. -
További szerzők:Marcheselli, Fiorella Moroni, Fausto Gáspár Rezső (1944-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Pieri, Carlo
Internet cím:elektronikus változat
elektronikus változat
Borító:

11.

001-es BibID:BIBFORM004654
035-os BibID:(scopus)0344199417 (wos)000083351400015
Első szerző:Varga Tamás (biológus)
Cím:Single-strand breaks in agarose-embedded chromatin of nonapoptotic cells / Varga, T., Szilagyi, I., Szabo, G.
Dátum:1999
Megjegyzések:Loop-size chromatin fragmentation frequently observed upon apoptotic cell death is thought to be initiated by ss nicks. Here we show that the agarose-embedded, deproteinized chromatin of normal, non-apoptotic murine and human cells, as well as yeast protoplasts, falls apart to 50-300 kb ss fragments upon heat denaturation, as revealed by urea-TAE field-inversion agarose gel electrophoresis resolving ss and ds fragments alike. These data were in line with S1digestion experiments. The nicks (gaps) observed are best explained either by enzymatic cleavages occurring upon cell lysis instantaneously or by preexisting discontinuities becoming manifest upon heat denaturation. These discontinuities go unnoticed in the usual nondenaturaing circumstances but seem to be inevitably present in any DNA preparation. The loop-size ds DNA fragmentation in apoptosis may be based on these pre-existing or "ready-to-go" (upon cell lysis) ss discontinuities of the normal cellular chromatin
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Animals
Apoptosis
Aspergillus Nuclease S1
Biophysics
Cell Line
Cells
chemistry
Chromatin
Dna
DNA Damage
DNA Fragmentation
DNA,Single-Stranded
Electrophoresis, Agar Gel
Electrophoresis, Gel, Pulsed-Field
Heat
Human
Humans
Hungary
Mice
Nucleic Acid Denaturation
Research
Saccharomyces cerevisiae
Sepharose
Support
ultrastructure
Urea
Megjelenés:Biochemical and Biophysical Research Communications. - 264 : 2 (1999), p. 388-394. -
További szerzők:Szilágyi Ildikó (orvos) Szabó Gábor (1953-) (biofizikus)
Internet cím:DOI
elektronikus változat
Borító:
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