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001-es BibID:	BIBFORM016665
Első szerző:	Balajthy Zoltán (biokémikus, sejtbiológus)
Cím:	Tissue-transglutaminase contributes to neutrophil granulocyte differentiation and functions / Zoltán Balajthy, Krisztián Csomós, György Vámosi, Attila Szántó, Michel Lanotte, László Fésüs
Dátum:	2006
ISSN:	0006-4971
Megjegyzések:	Promyelocytic NB4 leukemia cells undergo differentiation to granulocytes following retinoic acid treatment. Here we report that tissue transglutaminase (TG2), a protein cross-linking enzyme, was induced, then partially translocated into the nucleus, and became strongly associated with the chromatin during the differentiation process. The transglutaminase-catalyzed cross-link content of both the cytosolic and the nuclear protein fractions increased while NB4 cells underwent cellular maturation. Inhibition of cross-linking activity of TG2 by monodansylcadaverin in these cells led to diminished nitroblue tetrazolium (NBT) positivity, production of less superoxide anion, and decreased expression of GP91PHOX, the membrane-associated subunit of NADPH oxidase. Neutrophils isolated from TG2(-/-) mice showed diminished NBT reduction capacity, reduced superoxide anion formation, and down-regulation of the gp91phox subunit of NADPH oxidase, compared with wild-type cells. It was also observed that TG2(-/-) mice exhibited increased neutrophil phagocytic activity, but had attenuated neutrophil chemotaxis and impaired neutrophil extravasation with higher neutrophil counts in their circulation during yeast extract-induced peritonitis. These results clearly suggest that TG2 may modulate the expression of genes related to neutrophil functions and is involved in several intracellular and extracellular functions of extravasating neutrophil.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Blood. - 108 : 6 (2006), p. 2045-2054. -
További szerzők:	Csomós Krisztián (1981-) (molekuláris biológus) Vámosi György (1967-) (biofizikus) Szántó Attila (1976-) (orvos, biokémikus) Lanotte, Michel Fésüs László (1947-) (orvos biokémikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM004714
Első szerző:	Novák Levente (biológus)
Cím:	Shear-dependent morphology of von Willebrand factor bound to immobilized collagen / Novak, L., Deckmyn, H., Damjanovich, S., Harsfalvi, J.
Dátum:	2002
Megjegyzések:	We have developed an immunogold von Willebrand factor (VWF) detection method that permits almost complete coverage of individual VWF molecules, and by this unequivocal localization and morphologic analysis of collagen-bound VWF by atomic force microscopy (AFM). Perfusion of gel filtration-purified VWF in parallel plate perfusion chambers over glass coverslips coated with calf skin collagen, followed by AFM imaging in air, enabled us to assess possible morphologic differences between VWF bound at low (0.07 N/m(2) = 0.7 dynes/cm(2)) and high (4.55 N/m(2) = 45.5 dynes/cm(2)) shear stresses. No significant differences in VWF morphology were found, the molecules were oriented almost randomly, and there were no clear signs of VWF "uncoiling" either at a high or at a low shear regime. After perfusing 1 microg/mL VWF for 5 minutes, surface coverage at high shear was almost twice the one seen at low shear, and some larger and more irregularly shaped VWF molecules could be seen at high shear. This difference disappeared, however, at 15 minutes of perfusion and was probably caused by diffusion kinetics. Moreover, the presence of 68 x 10(9)/L washed fixed platelets in the perfusate did not have any visible effect on VWF morphology at high versus low shear stress. These findings suggest that shear stress does not influence significantly the overall molecular morphology of VWF during its binding to collagen-coated surface and are consistent with a constitutively expressed affinity of collagen-bound VWF for glycoprotein Ib.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Animal Blood Platelets Cattle chemistry Collagen Type I Diffusion Human Hungary Immunohistochemistry Kinetics metabolism methods Microscopy Microscopy,Atomic Force pathology Perfusion Platelet Adhesiveness Protein Binding Stress,Mechanical Support,Non-U.S.Gov't ultrastructure von Willebrand Factor
Megjelenés:	Blood. - 99 : 6 (2002), p. 2070-2076. -
További szerzők:	Deckmyn, Hans Damjanovich Sándor (1936-2017) (biofizikus) Hársfalvi Jolán (1949-) (klinikai biokémikus)
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