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001-es BibID:BIBFORM006054
Első szerző:Szöllősi János (biofizikus)
Cím:Calcium channels in PDGF-stimulated A172 cells open after intracellular calcium release and are not voltage-dependent / Szollosi, J., Feuerstein, B. G., Vereb, G., Pershadsingh, H. A., Marton, L. J.
Dátum:1991
Megjegyzések:Using laser image cytometry and Indo-1 fluorescence, we investigated the intracellular free Ca2+ concentration ([Ca2+]i) of confluent A172 human glioblastoma cells stimulated by the BB homodimer of platelet-derived growth factor (PDGF-BB). The shape of the calcium transients and the delay time between stimulation and the beginning of the transient varied considerably. The percentage of responsive cells, the peak [Ca2+]i and the duration of the response were directly related to PDGF-BB dose, while the delay time was inversely related; the maximal response occurred at a PDGF-BB concentration of 20 ng/ml. Studies with EGTA and inorganic calcium-channel blockers (Ni2+, La3+) showed that the increase of [Ca2+]i resulted from initial release of intracellular stores and subsequent calcium influx across the plasma membrane. Opening of calcium channels in the plasma membrane, monitored directly by studying Mn2+ quenching of Indo-1 fluorescence, was stimulated by PDGF-BB and blocked by La3+; the opening occurred 55 +/- 10 s after the initial increase in [Ca2+]i. Therefore, in these tumor cells, intracellular release always occurs before channel opening in the plasma membrane. Depolarization of cells with high extracellular [K+] did not generally induce calcium transients but did decrease calcium influx. L-type calcium-channel blockers (verapamil, nifedipine, and diltiazem) had little or no effect on the calcium influx induced by PDGF-BB. These results indicate that PDGF-BB induces calcium influx by a mechanism independent of voltage-sensitive calcium channels in A172 human glioblastoma cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Calcium
Calcium Channel Blockers
Calcium Channels
Dyes
Egtazic Acid
Electrophysiology
Fluorescence
Fluorescent Dyes
Glioblastoma
Human
Image Cytometry
Image Processing,Computer-Assisted
Indoles
Lanthanum
metabolism
pharmacology
Platelet-Derived Growth Factor
Signal Transduction
Support,Non-U.S.Gov't
Support,U.S.Gov't,P.H.S.
Tumor Cells,Cultured
Verapamil
Megjelenés:Cell Calcium. - 12 : 7 (1991), p. 477-491. -
További szerzők:Feuerstein, Burt G. Vereb György (1965-) (biofizikus, orvos) Pershadsingh, Harrihar A. Marton, Laurence J.
Internet cím:elektronikus változat
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