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001-es BibID:	BIBFORM005962
Első szerző:	Ábel György
Cím:	Effect of lentinan and mannan on phagocytosis of fluorescent latex microbeads by mouse peritoneal macrophages : a flow cytometric study / György Ábel, János Szöllösi, Goro Chihara, József Fachet
Dátum:	1989
Megjegyzések:	Lentinan, an immunopotentiating beta-1,3-glucan polysaccharide stimulated the in vitro phagocytosis of BSA-coated, C3b- or monoclonal immunoglobulin (IgG2b)-coated fluorescent microspheres by resident or thioglycollate-elicited mouse macrophages in a dose-dependent manner. Analysis of flow cytometric data has shown that microbead phagocytosis of resident macrophages, which exhibit a lower basic phagocytic activity than the thioglycollate elicited ones, has been augmented by up to 900% due to lentinan. The percent ratio of phagocytes among peritoneal exudate cells, however, remained unchanged after short-term lentinan stimulation. Preincubation of the cells with lentinan resulted in increased ingestion of the microbeads. Activation of phagocytosis by lentinan is therefore due in part to the direct stimulation of the cells, however, lentinan also serves as supplementary opsonin for C3b-coated beads. Mannan inhibited the ingestion of C3b-coated microspheres by 75%, which was abolished in part when lentinan was also added to the cells. Mannan did not influence the phagocytosis of BSA-coated or IgG-coated beads. Our data, based solely on in vitro studies, suggest a beta-glucan receptor mediated activation of phagocytes by lentinan. These receptors are different from the C3b, Fc or mannose receptors. It is very likely that stimulation of phagocytic activity of macrophages by lentinan may contribute to the antitumor action of this immunopotentiating polysaccharide.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Animal Antibodies Antibodies,Monoclonal Complement 3 cytology diagnostic use drug effects Dyes Female Flow Cytometry Fluorescent Dyes Hungary immunology In Vitro Latex Lentinan Macrophages Mannans Mice Microspheres Peritoneal Cavity Phagocytosis pharmacology Polysaccharides Thioglycolates
Megjelenés:	International Journal of Immunopharmacology. - 11 : 6 (1989), p. 615-621. -
További szerzők:	Szöllősi János (1953-) (biofizikus) Chihara, Goro Fachet József (1935-2016) (immunológus, kórélettanász)
Internet cím:	elektronikus változat
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001-es BibID:	BIBFORM005961
Első szerző:	Ábel György
Cím:	Effect of lentinan on pinocytosis in mouse peritoneal macrophages and the murine macrophage cell line C4M phi in vitro / György Ábel, János Szöllösi, Goro Chihara, József Fachet
Dátum:	1986
Megjegyzések:	Lentinan, an immunopotentiating polysaccharide, stimulated the pinocytosis of horseradish peroxidase (HRP) or FITC-dextran by resident or thioglycollate-elicited mouse macrophages from 10 to 50% in a dose dependent manner. Pinocytosis of HRP and FITC-dextran by C4M phi cells, a murine macrophage cell line, exhibiting a lower basic pinocytic activity than peritoneal cells, was augmented up to 310 and 120%, respectively, by lentinan. Mannan inhibited the HRP uptake by peritoneal macrophages via specific mannose receptors. This inhibitory effect was partly abolished, when lentinan was also added to the cells. Mannan was not able to inhibit pinocytosis of HRP by C4M phi macrophages, indicating little or no mannose receptor activity on these cells. Pinocytosis of FITC-dextran was not affected by mannan. Lentinan, opsonized in mouse sera inhibited the uptake of HRP by peritoneal macrophages by 30-35%. Opsonized lentinan and mannan added together caused 60% inhibition of HRP uptake in peritoneal macrophages indicating a possible functional relationship between the mannose and C3b receptors. The results demonstrate that lentinan activates the pinocytic function of macrophages predominantly via specific beta-glucan receptors. These mechanisms may contribute to the antitumor and immunopotentiating action of lentinan and other glucan-type polysaccharides.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Adjuvants,Immunologic Animal Cell Line drug effects Female immunology In Vitro Lentinan Macrophage Activation Macrophages Mice Mice,Inbred BALB C pharmacology Pinocytosis Polysaccharides
Megjelenés:	International Journal of Immunopharmacology. - 8 : 8 (1986), p. 919-924. -
További szerzők:	Szöllősi János (1953-) (biofizikus) Chihara, Goro Fachet József (1935-2016) (immunológus, kórélettanász)
Internet cím:	elektronikus változat
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