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1.

001-es BibID:BIBFORM004659
Első szerző:Dzoljic, M.
Cím:Ethanol and halothane differently modulate HLA class I and class II oligomerization : a new look at the mode of action of anesthetic agents through fluorescence spectroscopy / Dzoljic, M., Bene, L., Krasznai, Z., Damjanovich, S., Van Duijn, B.
Dátum:2000
Megjegyzések:The field of research considering the working mechanism of anesthetic agents is a complex one and the site or sites of action of general anesthetics are yet to be elucidated. Through the years, on the molecular level, the discussion has shifted from the lipid theories to the more specific interaction with the proteins responsible for the signal transduction. While this approach led to several models, they offer, at best, partial explanations for the observed phenomena. Anesthetic agents interact with many systems, of which the neuronal is best studied, leaving interaction with the immune defense system relatively unexplored. In this study we focus on the interaction of ethanol and halothane with the co-localization on the membrane of HLA I and II molecules. We show that ethanol tends to randomize the distribution of HLA I and II molecules, while halothane increases the clustering of HLA I proteins. The notion that anesthetics modulate cell function by disrupting clustering and thereby promoting a random distribution is a novel approach that may explain the general involvement of many systems during exposition to anesthetic drugs. In this study we show the disturbance of co-localization of molecules that may form a functional network. The relevance of this finding depends on the importance of these networks for extracellular and intracellular processes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Anesthetics, Inhalation
Antibodies, Monoclonal
B-Lymphocytes
Cell Line
chemistry
Comparative Study
drug effects
Energy Transfer
Ethanol
Fluorescence
Halothane
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Human
Macromolecular Systems
methods
pharmacology
Research
Signal Transduction
Spectrometry, Fluorescence
Support, Non-U.S.Gov't
Megjelenés:Journal of Photochemistry and Photobiology. B, Biology. - 56 : 1 (2000), p. 48-52. -
További szerzők:Bene László (1963-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Duijn, B., Van
Internet cím:DOI
elektronikus változat
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2.

001-es BibID:BIBFORM046295
Első szerző:Goda Katalin (biofizikus)
Cím:Intracellular pH does not affect drug extrusion by P-glycoprotein / Goda Katalin, Balkay László, Márián Teréz, Trón Lajos, Aszalós Adorján, Szabó Gábor
Dátum:1996
ISSN:1011-1344
Megjegyzések:The intracellular pH (pH(i)) of cells exhibiting multidrug resistance (MDR) related to the expression of the P-glycoprotein (Pgp) is often more alkaline than that of the parental cells, as also observed for the KB-V1/KB-3-1 system in this paper. The possible role of an elevated pH(i) in Pgp-related MDR has been investigated by shifting back the pH(i) of the MDR+ cells to a more acidic value using the mobile proton ionophore carbonylcyanide m-chlorophenylhydrazone (CCCP). The influence of CCCP-evoked delta pH(i) on relative daunorubicin (DNR) accumulation was similar in the case of several Pgp positive and negative cell lines, in view of flow cytometric and radioactive drug accumulation studies and measuring DNR levels in the medium in a flow-through system. Our data argue against a significant effect of pH(i) on Pgp pumping efficiency. However, an indirect connection between pH(i) regulation and the MDR phenotype is suggested by the fact that acidification of the external medium in the presence of verpamil could be observed exclusively in MDR+ cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Photochemistry And Photobiology B: Biology. - 34 : 2-3 (1996), p. 177-182. -
További szerzők:Balkay László (1963-) (biofizikus) Márián Teréz (1950-) (radiobiológus) Trón Lajos (1941-) (biofizikus) Aszalos Adorján Szabó Gábor (1953-) (biofizikus)
Pályázati támogatás:T017592
OTKA
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DOI
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3.

001-es BibID:BIBFORM046276
Első szerző:Krasznai Zoltán (biofizikus)
Cím:Flow cytometric determination of absolute membrane potential of cells / Krasznai Z., Marian T., Balkay L., Emri M., Tron L.
Dátum:1995
Megjegyzések:Membrane potential measurements using fluorescent membrane potential indicator dyes report on relative changes but usually do not result in an absolute value of the measured parameter. The method developed in this paper is based on the assumption that the negatively charged bis-oxonol distributes across the cytoplasmic membrane according to the Nernst equation. It is further supposed that the fluorescence intensity measured from a given stained cell is a single-value function of the intracellular dye concentration. The protocol suggested incorporates the construction of a calibration curve (fluorescence intensity measured from stained cells vs. extracellular dye concentration). This allows the evaluation of the membrane potential in millivolts using fluorescence readings of the cells both in the depolarized state and in the state of interest. Good agreement was found between absolute membrane potential data of human peripheral blood lymphocytes by our method and results of parallel patch clamp measurements.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of Photochemistry and Photobiology. B, Biology. - 28 : 1 (1995), p. 93-99. -
További szerzők:Márián Teréz (1950-) (radiobiológus) Balkay László (1963-) (biofizikus) Emri Miklós (1962-) (fizikus) Trón Lajos (1941-) (biofizikus)
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4.

001-es BibID:BIBFORM006043
Első szerző:Matkó János (biológus)
Cím:Mapping of cell surface protein-patterns by combined fluorescence anisotropy and energy transfer measurements / Janos Matko, Attila Jenei, Laszlo Matyus, Marcel Ameloot, Sandor Damjanovich
Dátum:1993
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
Biophysics
Cell Membrane
chemistry
Energy Transfer
Fluorescence
Fluorescence Polarization
Hungary
Mathematics
Membrane Proteins
metabolism
Models,Theoretical
Proteins
Spectrometry,Fluorescence
Support,Non-U.S.Gov't
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Photochemistry and Photobiology B: Biology 19 : 1 (1993), p. 69-73. -
További szerzők:Jenei Attila (1966-) (biofizikus) Mátyus László (1956-) (biofizikus) Ameloot, Marcel Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
DOI
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5.

001-es BibID:BIBFORM006045
Első szerző:Mátyus László (biofizikus)
Cím:Fluorescence resonance energy transfer measurements on cell surfaces. A spectroscopic tool for determining protein interactions / László Mátyus
Dátum:1992
Megjegyzések:The interaction of cell surface components may influence several events during the process of transmembrane signalling. Receptor clustering, conformational changes and altered molecular interactions often play essential roles in the final outcome of ligand receptor interactions. Fluorescence resonance energy transfer (FRET) is an excellent tool which can be used to determine distance relationships and supramolecular structure on cell surfaces. This paper reviews the theoretical basis of fluorescence resonance energy transfer, its spectrofluorometric and flow cytometric applications, and provides a critical evaluation of the methods. Finally, examples are given to illustrate the use of the method of fluorescence resonance energy transfer in solving biological problems.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
Biophysics
Cell Membrane
Dyes
Energy Transfer
Flow Cytometry
Fluorescence
Fluorescent Dyes
Hungary
Mathematics
Membrane Proteins
metabolism
methods
Models,Biological
Proteins
Spectrometry,Fluorescence
Support,Non-U.S.Gov't
Megjelenés:Journal of Photochemistry and Photobiology B: Biology. - 12 : 4 (1992), p. 323-337. -
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6.

001-es BibID:BIBFORM005089
Első szerző:Mátyus László (biofizikus)
Cím:Steady-state fluorescence quenching applications for studying protein structure and dynamics / Matyus, L., Szollosi, J., Jenei, A.
Dátum:2006
ISSN:011-1344 (Print)
Megjegyzések:Fluorescence quenching methods are useful to obtain information about the conformational and/or dynamic changes of proteins in complex macromolecular systems. In this review steady-state methods are described and the data interpretation is thoroughly discussed. As a special case of fluorescence quenching mechanism, fluorescence resonance energy transfer (FRET) phenomenon is also presented. Application of a FRET based method to characterize the temperature dependence of the flexibility of protein matrix is clearly demonstrated.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Acrylamide
Algorithms
Biophysics
Cesium
chemistry
Chlorides
Energy Transfer
Fluorescence
Fluorescence Resonance Energy Transfer
Hungary
Macromolecular Systems
metabolism
methods
Protein Conformation
Proteins
Research
Support
Temperature
Tryptophan
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Photochemistry and Photobiology. B, Biology. - 83 : 3 (2006), p. 223-236. -
További szerzők:Szöllősi János (1953-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Internet cím:elektronikus változat
DOI
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7.

001-es BibID:BIBFORM004685
Első szerző:Mátyus László (biofizikus)
Cím:Organization of the glycoprotein (GP) IIb/IIIa heterodimer on resting human platelets studied by flow cytometric energy transfer / Matyus, L., Bene, L., Harsfalvi, J., Alvarez, M. V., Gonzalez-Rodriguez, J., Jenei, A., Muszbek, L., Damjanovich, S.
Dátum:2001
Megjegyzések:Glycoprotein IIb/IIIa is a heterodimer of glycoproteins IIb and IIIa which serves as the inducible receptor for fibrinogen and other adhesive proteins at the surface of platelets. Although a model of the quaternary structure of the GPIIb/IIIa molecule has been constructed in solution by Calvete et al. [Biochem. J. 282 (1992) 523], a corresponding model at the surface of intact platelets is still missing. In the present work conformation and lateral distribution of the GPIIb/IIIa heterodimer were studied at a nanometer resolution on the surface of resting human platelets under physiological conditions. The experiments were based on dual wavelength flow cytometric detection of fluorescence resonance energy transfer and application of a panel of monoclonal antibodies raised against well described binding sites. Monodisperse distribution of the GPIIb/IIIa heterodimer has been observed and a detailed three-dimensional proximity map of antibody binding sites was constructed on the platelet membrane, under physiological conditions, for the first time. Our data support the view that the GPIIb subunit is in a bent conformation. A detailed analysis of the K(d)-values and the number of binding sites for a set of monoclonal antibodies was also carried out giving supplementary data for the topology of the binding sites. Our results provide a refinement of the membrane-topology of the GPIIb/IIIa heterodimer.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Animal
Antibodies,Monoclonal
Binding Sites
Blood Platelets
Dimerization
Energy Transfer
Flow Cytometry
Fluorescence
Glycoproteins
Human
Hungary
metabolism
Mice
Platelet Glycoprotein GPIIb-IIIa Complex
Support, Non-U.S.Gov't
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Photochemistry and Photobiology. B, Biology. - 65 : 1 (2001), p. 47-58. -
További szerzők:Bene László (1963-) (biofizikus) Hársfalvi Jolán (1949-) (klinikai biokémikus) Alvarez, M. V. Gonzalez-Rodriguez, J. Jenei Attila (1966-) (biofizikus) Muszbek László (1942-) (haematológus, kutató orvos) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:DOI
elektronikus változat
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8.

001-es BibID:BIBFORM116504
035-os BibID:(cikkazonosító)112785 (scopus)85170688343 (wos)001081473700001
Első szerző:Pevná, Viktória
Cím:Effective transport of aggregated hypericin encapsulated in SBA-15 nanoporous silica particles for photodynamic therapy of cancer cells / Pevná Viktória, Zauska Lubos, Benziane Anass, Vámosi György, Girman Vladimír, Miklósová Monika, Zelenák Vladimír, Huntosová Veronika, Almási Miroslav
Dátum:2023
ISSN:1011-1344 1873-2682
Megjegyzések:Photodynamic therapy (PDT) represents an interesting modality for the elimination of damaged biomaterials and cells. This treatment takes advantage of the photosensitizing properties of molecules that are active only when irradiated with light. In the present work, a dual property of hypericin, a hydrophobic molecule with high performance in photodiagnostics and photodynamic therapy, was exploited. The non-fluorescent and photodynamically inactive form of hypericin aggregates was loaded into the nanopores of SBA-15 silica particles. The synthesized particles were characterized by infrared spectroscopy, thermogravimetry, differential thermal analysis, small-angle X-ray scattering and transmission electron microscopy. Hypericin aggregates were confirmed by absorption spectra typical of aggregated hypericin and by its short fluorescence lifetime. Release of hypericin from the particles was observed toward serum proteins, mimicking physiological conditions. Temperature- and time-dependent uptake of hypericin by cancer cells showed gradual release of hypericin from the particles and active cellular transport by endocytosis. A closer examination of SBA-15-hypericin uptake by fluorescence lifetime imaging showed that aggregated hypericin molecules, characterized by a short fluorescence lifetime (?4 ns), were still present in the SBA-15 particles upon uptake by cells. However, monomerization of hypericin in cancer cells was observed by extending the hypericin fluorescence lifetime by ?8 ns, preferentially in lipid compartments and the plasma membrane. This suggests a promising prognosis for delayed biological activity of the entire cargo, which was confirmed by effective PDT in vitro. In summary, this work presents an approach for safe, inactive delivery of hypericin that is activated at the target site in cells and tissues.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Nanoporous silica
Hypericin
Transport
Cancer cells
Fluorescence lifetime
Photodynamic therapy
Megjelenés:Journal Of Photochemistry And Photobiology B-Biology. - 247 (2023), p. 1-11. -
További szerzők:Zauška, Ľuboš Benziane, Anass (1990-) (molekuláris biológus) Vámosi György (1967-) (biofizikus) Girman, Vladimír Miklóšová, Monika Zeleňák, Vladimír Huntošová, Veronika Almáši, Miroslav
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DOI
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9.

001-es BibID:BIBFORM004936
Első szerző:Vereb György (biofizikus, orvos)
Cím:Immobilization of molecules, membranes and cells for modern optical and non-optical microscopy by photo-cross-linking / Vereb, G. Jr., Damjanovich, S., Jovin, T. M.
Dátum:1995
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Atomic force microscope.Adhesion.Surface
Cells
Microscopy
Megjelenés:Journal of Photochemistry and Photobiology. B, Biology. - 27 : 3 (1995), p. 275-277. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Jovin, Thomas M.
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