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001-es BibID:	BIBFORM004631
Első szerző:	Gáspár Rezső (biofizikus)
Cím:	Evidence of non-synaptic regulation of postpartum uterine contractility in the rat / Gaspar, R., Marki, A., Zupko, I., Falkay, G.
Dátum:	1998
ISSN:	024-3205
Megjegyzések:	Myometrial tissue rings from postpartum rats (24 h after delivery) were studied in vitro by electric field stimulation, and the alpha1/beta2-adrenoceptor ratio was determined by a radioligand binding technique. Pregnancy-denervated uterine rings were stimulated by long-duration pulses (100 ms). The contractions were inhibited by beta2-agonists (terbutaline and fenoterol) and alpha-antagonists (phentolamine, urapidil and yohimbine) in a concentration-dependent manner. Their effects were not altered by the adrenergic neuron-blocking agent bretylium. The alpha-antagonists (except phentolamine) elicited the same maximal inhibition as the beta2-agonists. Receptor assays revealed that the alpha1/beta2 ratio was about 2 in the measured uteri. It was concluded that the inhibitory effects of alpha-antagonists and beta2-agonists are mediated via non-synaptic adrenoceptors of the denervated postpartum rat uterus. The same inhibitory activity could be explained by the greater amount of alpha-receptors. It is believed that this is the first functional proof of the existence of non-synaptic alpha1-adrenoceptors in smooth muscle.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Adrenergic beta-Agonists Animal Binding Sites Electric Stimulation Female Fenoterol Hungary In Vitro Male Myometrium pharmacology physiology Puerperium Radioligand Assay Rats Rats, Sprague-Dawley Receptors, Adrenergic, alpha Receptors, Adrenergic, alpha-1 Receptors, Adrenergic, beta Support, Non-U.S.Gov't Synapses Terbutaline ultrastructure Uterine Contraction Uterus
Megjelenés:	Life Sciences. - 62 : 12 (1998), p. 1119-1124. -
További szerzők:	Márki A. Zupkó István Falkay György
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001-es BibID:	BIBFORM004729
Első szerző:	Heilmeyer, Ludwig M. G. Jr.
Cím:	Mammalian phosphatidylinositol 4-kinases / Heilmeyer, L. M. G. Jr., Vereb, G. Jr., Vereb, G., Kakuk, A., Szivak, I.
Dátum:	2003
ISSN:	521-6543 (Print)
Megjegyzések:	Three phosphatidylinositol 4-kinase isoforms, PI4K 230, 92 and 55 have been cloned and sequenced allowing a much wider characterization than the previously employed enzymological typing into type II and III enzymes. PI4K 230 and 92 contain a highly conserved catalytic core, PI4K55 one with a much lower degree of similarity. Candidate kinase motifs, deduced from the protein kinase super family, are absolutely conserved in all isoforms. Kinase activities are described based on their sensitivity and reactivity towards wortmannin, phenylarsine oxide (PAO) and 5'-p-fluorosulfonylbenzoyladenosine (FSBA). Localization of all isoforms in the cell is reported. All enzymes contain nuclear localization and export sequence motifs (NLS and NES) leading to the expectation that they can be transferred to the nucleus. PI4K230 has been found in the nucleolus, PI4K92 in the nucleus, additionally further broadening the function of these enzymes. In the cytoplasm of neuronal cells, PI4K230 is distributed evenly on membranes that are ultra structurally cisterns of the rough endoplasmatic reticulum, outer membranes of mitochondria, multivesicular bodies, and are in close vicinity of synaptic contacts. PI4K92 is functionally characterized as a key enzyme regulating Golgi disintegration/reorganization during mitosis probably via phosphorylation by cyclin-dependent kinases on well-defined sites. PI4K55 is involved in the production of second messengers, diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (InsP3) at the plasma membrane, moreover, in the endocytotic pathway in the cytoplasm
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban 1-Phosphatidylinositol 4-Kinase Animals Cells Cytoplasm Enzymes genetics Humans Inositol 1,4,5-Trisphosphate metabolism Mitochondria Mitosis Phosphorylation Protein Isoforms Rats Research Support
Megjelenés:	IUBMB Life 55 : 2 (2003), p. 59-65. -
További szerzők:	Vereb György (1965-) (biofizikus, orvos) Vereb György (1938-) (biokémikus, sejtbiológus) Kakuk Annamária (1976-) (molekuláris biológus) Szivák Ilona
Internet cím:	elektronikus változat elektronikus változat DOI
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001-es BibID:	BIBFORM099098
035-os BibID:	(cikkazonosító)1296
Első szerző:	Káldy Jenő
Cím:	Effects of Hydrostatic Pressure Treatment of Newly Fertilized Eggs on the Ploidy Level and Karyotype of Pikeperch Sander lucioperca (Linnaeus, 1758) / Káldy Jenő, Patakiné Várkonyi Eszter, Fazekas Georgina Lea, Nagy Zoltán, Sándor Zsuzsanna J., Bogár Katalin, Kovács Gyula, Molnár Mariann, Lázár Bence, Goda Katalin, Gyöngy Zsuzsanna, Ritter Zsuzsanna, Nánási Péter, Horváth Ákos, Ljubobratović Uroŝ
Dátum:	2021
ISSN:	2075-1729
Megjegyzések:	We studied the effect of different magnitudes (7000 PSI (48.26 MPa), 8000 PSI (55.16 MPa), and 9000 PSI (62.05 MPa)) of hydrostatic pressure on the ploidy of pikeperch larvae. Pressure shock was applied 5 min after the fertilization of eggs at a water temperature of 14.8 ? 1 ?C. A 7000 PSI pressure shock was applied for 10 or 20 min, while 8000 and 9000 PSI treatments lasted for 10 min. Each treatment with its respective control was completed in triplicate, where different females' eggs served as a replicate. In the treatment groups exposed to 7000 PSI for 10 min, only diploid and triploid larvae were identified, while 2n/3n mosaic individuals were found after a 20-min exposure to a 7000 PSI pressure shock. The application of 8000 or 9000 PSI pressure shocks resulted in only triploid and mosaic individuals. Among larvae from eggs treated with 8000 PSI, three mosaic individuals with 2n/3n karyotype were identified (4.0 ? 6.9%), while a single (2.0 ? 3.5%) 1n/3n mosaic individual was found in the 9000 PSI-treated group. To our knowledge, this is the first report that demonstrates the induction of a haplo-triploid karyotype by hydrostatic pressure shock in teleost fish. The dominance of triploid individuals with a reasonable survival rate (36.8 ? 26.1%) after 8000 PSI shock supports the suitability of the hydrostatic pressure treatment of freshly fertilized eggs for triploid induction in pikeperch.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk triploid mosaic karyotype pressure duration chromosome number PSI
Megjelenés:	Life. - 11 : 12 (2021), p. 1-11. -
További szerzők:	Patakiné Várkonyi Eszter Fazekas Georgina Nagy Zoltán Jakabné Sándor Zsuzsanna Bogár Katalin Kovács Gyula Molnár Marianna Lázár Bence Goda Katalin (1969-) (biofizikus) Gyöngy Zsuzsanna (1991-) (molekuláris biológus) Ritter Zsuzsanna Nánási Péter Pál ifj. (1987-) (sejtbiológus) Horváth Ákos Ljubobratović, Uroš
Pályázati támogatás:	K124815 OTKA
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001-es BibID:	BIBFORM091923
Első szerző:	Kaňa, Radek
Cím:	Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane / Radek Kaňa, Gábor Steinbach, Roman Sobotka, György Vámosi, Josef Komenda
Dátum:	2020
ISSN:	2075-1729
Megjegyzések:	Biological membranes were originally described as a fluid mosaic with uniform distribution of proteins and lipids. Later, heterogeneous membrane areas were found in many membrane systems including cyanobacterial thylakoids. In fact, cyanobacterial pigment?protein complexes (photosystems, phycobilisomes) form a heterogeneous mosaic of thylakoid membrane microdomains (MDs) restricting protein mobility. The trafficking of membrane proteins is one of the key factors for long-term survival under stress conditions, for instance during exposure to photoinhibitory light conditions. However, the mobility of unbound ♭free' proteins in thylakoid membrane is poorly characterized. In this work, we assessed the maximal diffusional ability of a small, unbound thylakoid membrane protein by semi-single molecule FCS (fluorescence correlation spectroscopy) method in the cyanobacterium Synechocystis sp. PCC6803. We utilized a GFP-tagged variant of the cytochrome b6f subunit PetC1 (PetC1-GFP), which was not assembled in the b6f complex due to the presence of the tag. Subsequent FCS measurements have identified a very fast diffusion of the PetC1-GFP protein in the thylakoid membrane (D = 0.14 ?? 2.95 m2s??1). This means that the mobility of PetC1-GFP was comparable with that of free lipids and was 50?500 times higher in comparison to the mobility of proteins (e.g., IsiA, LHCII?light-harvesting complexes of PSII) naturally associated with larger thylakoid membrane complexes like photosystems. Our results thus demonstrate the ability of free thylakoid-membrane proteins to move very fast, revealing the crucial role of protein?protein interactions in the mobility restrictions for large thylakoid protein complexes.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk proteins mobility photosynthesis FCS thylakoids cyanobacteria
Megjelenés:	Life. - 11 : 1 (2020), p. 1-12. -
További szerzők:	Steinbach Gábor Sobotka, Roman Vámosi György (1967-) (biofizikus) Komenda, Josef
Pályázati támogatás:	GINOP-2.3.2- 15-2016-00001 GINOP NKFIH NN 129371 Egyéb NKFIH ANN 135107 Egyéb
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