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1.

001-es BibID:BIBFORM005972
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Dynamic properties of the murine histocompatibility H-2Kk antigen in cytoplasmic membrane / S. Damjanovich, L. Tron, J. Szollosi, L. Matyus, G. Szabo
Dátum:1984
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Animal
Antigens,Surface
Biophysics
Cell Membrane
Concanavalin A
Epitopes
Fibroblasts
H-2 Antigens
Human
immunology
Lymphocytes
Lymphoma
metabolism
Mice
Mice,Inbred A
Receptors,Concanavalin A
Rotation
Megjelenés:Molecular Immunology. - 21 : 12 (1984), p. 1151-1155. -
További szerzők:Trón Lajos (1941-) (biofizikus) Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Szabó Gábor (1953-) (biofizikus)
Internet cím:elektronikus változat
DOI
Intézményi repozitóriumban (DEA) tárolt változa
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2.

001-es BibID:BIBFORM005967
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Cytoplasmic membrane potential of mouse lymphocytes is decreased by cyclosporins / Damjanovich, S., Aszalos, A., Mulhern, S., Balazs, M., Matyus, L.
Dátum:1986
Megjegyzések:Membrane potential of mouse lymphocytes was investigated in the presence and absence of cyclosporin A (CsA) and cyclosporin G (CsG) by flow cytometry and fluorescence spectroscopy. A carbocyanine dye, dihexyloxacarbocyanine iodide [DIOC6(3)], was applied as a membrane potential probe. A dose-dependent decrease in the membrane potential of T and B lymphocytes was observed in the presence of CsA and CsG. However, pretreatment of lymphocytes with insulin reduced the effect of the cyclosporins. Mobile ionophores, such as valinomycin, ionomycin and A23187 were less effective in changing the membrane potential of lymphocytes in the presence of CsA. The channel forming ionophore, gramicidin or high extra-cellular potassium concentration (160 mM) strongly reduced the membrane potential regardless of the absence or presence the CsA. These observations suggest incorporation of CsA into the cytoplasmic membrane causing changes in ion fluxes. Other reported biochemical effects of CsA may be secondary to the observed membrane potential changes. The membrane potential change induced by CsA may have selective biological consequences in a certain subpopulation of lymphocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
antagonists and inhibitors
B-Lymphocytes
Cyclosporins
drug effects
Flow Cytometry
Fluorescence
Insulin
Ionomycin
Ionophores
Lymphocytes
Membrane Potentials
Mice
Mice,Inbred Strains
pharmacology
physiology
Potassium
Spectrometry,Fluorescence
Valinomycin
Megjelenés:Molecular Immunology. - 23 : 2 (1986), p. 175-180. -
További szerzők:Aszalos Adorján Mulhern, Sally Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mátyus László (1956-) (biofizikus)
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3.

001-es BibID:BIBFORM006052
Első szerző:Pieri, Carlo
Cím:A sodium channel opener inhibits stimulation of human peripheral blood mononuclear cells / Pieri, C., Recchioni, R., Moroni, F., Marcheselli, F., Falasca, M., Krasznai, Z., Gaspar, R., Matyus, L., Damjanovich, S.
Dátum:1992
Megjegyzések:The role of membrane potential changes in T cell activation was studied on human peripheral blood lymphocytes stimulated with phytohemagglutinin. Addition of bretylium tosylate, a sodium channels opener, to PHA treated lymphocytes modified the membrane potential and consequently blocked cell activation in a dose-dependent fashion. BT was non-toxic even in long-term (72 hr) incubations. It was reversibly removable, and the removal restored the stimulatory effect of PHA. 3H-thymidine incorporation was blocked if BT was present during the first 20-24 hr of the mitogenic activation. The later BT was added after PHA, the less inhibition of proliferation was observed. BT hyperpolarized the lymphocytes also in the presence of PHA. BT hindered the depolarizing effect of high extracellular potassium concns. The sustained polarized state of the lymphocytes did not influence the intracellular calcium increase upon PHA treatment. IL-2 and transferrin receptor expression was not hindered by BT during PHA stimulation of lymphocytes. Addition of rIL-2 did not abolish the inhibitory effect of BT. According to cell-cycle analysis BT arrested the majority of the cells in G1 phase. It is suggested that cell activation demands the flexible maintenance of a relatively narrow membrane potential "window". Any sustained and significant hyper-, or depolarization, may dramatically decrease the effectivity of transmembrane signalling.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
blood
Bretylium Tosylate
Calcium
Cell Cycle
cytology
Dose-Response Relationship,Drug
drug effects
Flow Cytometry
Human
In Vitro
Interleukin-2
Lymphocyte Transformation
Lymphocytes
Membrane Potentials
pharmacology
physiology
Phytohemagglutinins
Potassium
Receptors,Transferrin
Research
Sodium
Sodium Channels
Support,Non-U.S.Gov't
T-Lymphocytes
Megjelenés:Molecular Immunology. - 29 : 4 (1992), p. 517-524. -
További szerzők:Recchioni, Rina Moroni, Fausto Marcheselli, Fiorella Falasca, Marco Krasznai Zoltán (1950-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Mátyus László (1956-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
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4.

001-es BibID:BIBFORM024107
Első szerző:Szabó Gábor (biofizikus)
Cím:Specific Disengagement of Cell-Bound Anti-Lam-1 (Anti-L-Selectin) Antibodies by Aurintricarboxylic Acid / Gábor Szabo Jr., James L. Weaver, P. Scott Pine, Adorján Aszalos
Dátum:1993
Megjegyzések:Brief treatment of human peripheral blood lymphocytes with the potential anti-HIV compound aurintricarboxylic acid (ATA) prompts the selective release of already bound L-selectin-specific anti-leu8 and anti-LAM1-1 antibodies from the cells. Two other anti-LAM1 antibodies, anti-LAM1-3 and anti-LAM1-5 stay antigen-bound at the same time. interestingly, the ATA-sensitive anti-leu8 strongly competes with the ATA-resistant anti-LAM1-3 for binding. Photobleaching fluorescence resonance energy transfer (pFRET) measurements on flow-sorted cells suggests that these two antibodies compete for the same epitope, while anti-LAM1-5-FITC and anti-Leu8-PE bind to distinct sites, although they also compete for binding. Combining the data on competition, pFRET and ATA effect, we suggest that the ATA sensitive anti-leu8 and resistant anti-LAM1-3 bind to overlapping but non-identical epitopes. This remarkably specific effect may be exploited for designing anti-inflammatory drugs that modulate leukocyte adhesion
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Antibodies
blood
Cells
Energy Transfer
Epitopes
Fluorescence
Fluorescence Resonance Energy Transfer
Human
lymphocyte
Lymphocytes
Photobleaching
time
Megjelenés:Molecular Immunology. - 30 : 18 (1993), p. 1689-1694. -
További szerzők:Weaver, James L. Pine, P. Scott Aszalos Adorján
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5.

001-es BibID:BIBFORM006056
Első szerző:Trón Lajos (biofizikus)
Cím:Bretylium causes a K(+)-Na+ pump activation that is independent of Na+/H+ exchange in depolarized rat, mouse and human lymphocytes / Tron, L., Pieri, C., Marian, T., Balkay, L., Emri, M., Damjanovich, S.
Dátum:1990
Megjegyzések:We have studied a bretylium tosylate induced increase of the membrane potentials of partially depolarized rat, mouse and human lymphocytes, using the potential sensitive dye, bis [1,3, dibutylbarbituric acid-(5) trimethine oxonol]. The extent of this repolarization is dose-dependent and decreased in magnitude as the temp was reduced from 37 degrees C to room temp. The repolarizing effect is inhibited by K(+)-Na(+)-pump blockers or lack of extracellular Na+. Sodium ion channel blockers are effective in abolishing repolarization only if applied prior to, or simultaneously with, bretylium. Activation of Na+/H+ exchange is not involved in the mechanism of the phenomenon as the latter is completely eliminated in the presence of 10 microM amiloride (concn of the diuretics having no measurable inhibition on the action of the exchanger). These data suggest that bretylium opens ligand- and voltage-gated Na+ channels, and repolarization occurs due to higher activity of the K(+)-Na(+)-pump stimulated by the enhanced intracellular Na+ accumulation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
Bretylium Compounds
Bretylium Tosylate
drug effects
Human
Hungary
In Vitro
Lymphocytes
Membrane Potentials
metabolism
Mice
Na(+)-K(+)-Exchanging ATPase
pharmacology
physiology
Potassium
Protons
Rats
Rats,Inbred Strains
Sodium
Support,Non-U.S.Gov't
Megjelenés:Molecular Immunology. - 27 : 12 (1990), p. 1307-1311. -
További szerzők:Pieri, Carlo Márián Teréz (1950-) (radiobiológus) Balkay László (1963-) (biofizikus) Emri Miklós (1962-) (fizikus) Damjanovich Sándor (1936-2017) (biofizikus)
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6.

001-es BibID:BIBFORM006018
Első szerző:Trón Lajos (biofizikus)
Cím:On the biophysics of transmembrane signalling / L. Trón, A. Aszalos, M. Balázs, Sally A. Mulhern, J. Szöllösi, S. Damjanovich
Dátum:1988
Megjegyzések:Transmembrane signalling involves a number of physical translocations, changes in proximity of membrane elements like receptor subunits, or sequestration of proteins from the membrane. The monitoring of such changes with flow cytometric energy transfer revealed a new putative subunit of the IL-2 receptor and a possible intermolecular interaction between HLA class I and class II antigens. Lateral diffusion of the components of the multi-subunit IL-2 receptor was also followed. Changes in the intracellular pH were considered as a measure of efficient signal transfer in a number of cases. An overview and critical comparison of data is presented in the paper.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Biophysics
Cell Membrane
Diffusion
Energy Transfer
HLA Antigens
Human
Hungary
immunology
Interleukin-2
physiology
Receptors,Interleukin-2
Signal Transduction
Support,Non-U.S.Gov't
Megjelenés:Molecular Immunology. - 25 : 11 (1988), p. 1075-1080. -
További szerzők:Aszalos Adorján Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mulhern, Sally Szöllősi János (1953-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
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