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1.

001-es BibID:BIBFORM064954
Első szerző:Balajthy András (általános orvos)
Cím:7DHC-induced changes of Kv1.3 operation contributes to modified T cell function in Smith-Lemli-Opitz syndrome / András Balajthy, Sándor Somodi, Zoltán Pethő, Mária Péter, Zoltán Varga, Gabriella P. Szabó, György Paragh, László Vígh, György Panyi, Péter Hajdu
Dátum:2016
ISSN:0031-6768
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Pflügers Archiv. - 468 : 8 (2016), p. 1403-1418. -
További szerzők:Somodi Sándor (1977-) (belgyógyász) Pethő Zoltán (1989-) (orvos) Péter Mária Varga Zoltán (1969-) (biofizikus, szakfordító) P. Szabó Gabriella (1975-) (csecsemő- és gyermekgyógyász, klinikai genetikus) Paragh György (1953-) (belgyógyász) Vígh László (orvos Szeged) Panyi György (1966-) (biofizikus) Hajdu Péter (1975-) (biofizikus)
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DOI
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2.

001-es BibID:BIBFORM040778
Első szerző:Batta József Tamás (fül-orr-gégész)
Cím:Active and passive behaviour in the regulation of stiffness of the lateral wall in outer hair cells of the guinea-pig / Batta, T. J., Panyi, G., Gaspar, R., Sziklai, I.
Dátum:2003
ISSN:0031-6768
Megjegyzések:The stiffness of the outer hair cell (OHC) lateral wall, measured by the micropipette aspiration technique, is non-linear, decreasing from the ciliary pole (stiffness parameter Sp 1.83+/-0.13 nN/microm n=10) towards the cell base (Sp 1.14+/-0.16 nN/microm, n=10) irrespective of the cochleoapical or cochleobasal origin of the cells. The length of the aspirated lateral wall segment was related exponentially to the duration of the applied negative pressure (6 cm H2O) in the synaptic region of the OHCs whereas an active, sigmoid component was observed between 30 and 60 s in the supranuclear regions. A significant increase of the midlateral wall stiffness (to 1.91+/-0.23 nN/microm; n=10) was observed in calcium-free medium and the sigmoid component of the response of the lateral wall was abolished. Salicylate (5 mM) had no significant effect on the active sigmoid behaviour of the lateral wall (n=10). Gadolinium (5 mM), a non-specific cation channel blocker, increased the stiffness of the lateral wall and attenuated the active component (n=10). The motor protein prestin thus does not seem to be involved in the active stiffness regulation seen in this study. A role for the cortical cytoskeleton in the regulation of stiffness seems reasonable according to our model. The mechanism may involve calcium-dependent metabolic modification of cytoskeletal or membrane proteins.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Pflugers Archiv. - 447 : 3 (2003), p. 328-336. -
További szerzők:Panyi György (1966-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Sziklai István (1954-) (fül-orr-gégész)
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3.

001-es BibID:BIBFORM004927
Első szerző:Gáspár Rezső (biofizikus)
Cím:Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts / Rezső Gáspár, A. Freek Weidema, Zoltán Krasznai, Peter J. Nijweide, Dirk L. Ypey
Dátum:1995
ISSN:0031-6768
Megjegyzések:A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than -28 +/- 4 mV from a holding potential of -80 mV. It peaked within 1-1.5 ms, and inactivated within 3.3-6.9 ms. The 50% inactivation voltage was -59 +/- 6 mV with a steepness factor of 0.11 +/- 0.06. The current disappeared with the removal of extracellular Na+ and was reversibly blocked by tetrodotoxin (K0.5 < 15 nM) but not by verapamil (< or = 100 microM). We conclude that this new current in embryonic chicken osteoclasts is a sodium current known from excitable cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
Chick Embryo
Culture Media
drug effects
Hungary
In Vitro
metabolism
Osteoclasts
Patch-Clamp Techniques
pharmacology
Sodium
Sodium Channels
Support,Non-U.S.Gov't
Tetrodotoxin
Verapamil
Megjelenés:Pflügers Archiv. - 430 : 4 (1995), p. 596-598. -
További szerzők:Weidema, Adam Freek (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Nijweide, Peter J. Ypey, Dirk L.
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4.

001-es BibID:BIBFORM004728
Első szerző:Hajdu Péter (biofizikus)
Cím:Cholesterol modifies the gating of Kv1.3 in human T lymphocytes / Péter Hajdú, Zoltán Varga, Carlo Pieri, György Panyi, Rezső Gáspár
Dátum:2003
ISSN:0031-6768
Megjegyzések:The Kv1.3 potassium channel that belongs to the Shaker family of voltage-gated K(+) channels plays a crucial role in the mitogenic response of T cells. Because it spans the cell membrane its function can be influenced by lipid-protein interactions. In order to study the effect of lipid-protein interactions on the functioning of Kv1.3 we manipulated the membrane cholesterol content in T cells mimicking various physiological conditions by means of the oligosaccharide methyl-beta-cyclodextrin (MbetaCD) and its cholesterol-saturated complex (MbetaCD/C). Fluorescence polarization anisotropy and peak current density were used to monitor the efficiency of cholesterol removal (MbetaCD) and loading (MbetaCD/C). Using whole-cell patch-clamp technique we determined the kinetic and steady-state parameters of activation and inactivation of the Kv1.3 currents under different treatment conditions. Upon elevation of cholesterol content by 1 or 1.5 mg/ml MbetaCD/C the rates of both activation and inactivation were slowed. Moreover, the increased cholesterol level in the membrane resulted in a biphasic activation curve. Cholesterol depletion with MbetaCD (0.95 and 1.425 mg/ml) caused no significant changes in the gating characteristics of Kv1.3. The equilibrium between the open and the closed states of the channels was affected by increased cholesterol content, but at the same time steady-state inactivation was unchanged. We argue that manipulation of membrane cholesterol changed both the kinetic properties of Kv1.3 and steady-state parameters of activation by modifying lipid-protein interactions
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
beta-Cyclodextrins
Biophysics
Cell Membrane
Cells
Cholesterol
Cyclodextrins
drug effects
Electric Conductivity
Fluorescence
Fluorescence Polarization
Human
Humans
Hungary
Ion Channel Gating
Kinetics
Kv1.3 Potassium Channel
Lymphocytes
Membrane Microdomains
Membrane Potentials
metabolism
pharmacology
physiology
Potassium
Potassium Channels
Potassium Channels,Voltage-Gated
Research
Support
T-Lymphocytes
Megjelenés:Pflügers Archiv. - 445 : 6 (2003), p. 674-682. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Pieri, Carlo Panyi György (1966-) (biofizikus) Gáspár Rezső (1944-) (biofizikus)
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5.

001-es BibID:BIBFORM036694
Első szerző:Hegyi Bence (élettanász)
Cím:Tetrodotoxin blocks L-type Ca2+ channels in canine ventricular cardiomyocytes / Bence Hegyi, László Bárándi, István Komáromi, Ferenc Papp, Balázs Horváth, János Magyar, Tamás Bányász, Zoltán Krasznai, Norbert Szentandrássy, Péter P. Nánási
Dátum:2012
ISSN:0031-6768
Megjegyzések:Tetrodotoxin (TTX) is believed to be the mostselective inhibitor of voltage-gated fast Na+ channels inexcitable tissues, including nerve, skeletal muscle, andheart, although TTX sensitivity of the latter is lower thanthe former by at least three orders of magnitude. In thepresent study, the TTX sensitivity of L-type Ca2+ current(ICa) was studied in isolated canine ventricular cells usingconventional voltage clamp and action potential voltageclamp techniques. TTX was found to block ICa in a reversiblemanner without altering inactivation kinetics of ICa.Fitting results to the Hill equation, an IC50 value of55?2 ?M was obtained with a Hill coefficient of unity(1.0?s0.04). The current was fully abolished by 1 ?Mnisoldipine, indicating that it was really ICa. Under actionpotential voltage clamp conditions, the TTX-sensitivecurrent displayed the typical fingerprint of ICa, whichwas absent in the presence of nisoldipine. Stick-and-ballmodels for Cav1.2 and Nav1.5 channel proteins wereconstructed to explain the differences observed betweenaction of TTX on cardiac ICa and INa. This is the firstreport demonstrating TTX to interact with L-type calciumcurrent in the heart.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Calcium channels
Dog heart
Sodium channels
Tetrodotoxin
Voltage clamp
Megjelenés:Pflügers Archiv. - 464 : 2 (2012), p. 167-174. -
További szerzők:Bárándi László (1984-) (élettanász) Komáromi István (1957-) (vegyész, molekuláris biológus, biokémikus) Papp Ferenc (1979-) (biofizikus) Horváth Balázs (1981-) (élettanász) Magyar János (1961-) (élettanász) Bányász Tamás (1960-) (élettanász) Krasznai Zoltán (1950-) (biofizikus) Szentandrássy Norbert (1976-) (élettanász) Nánási Péter Pál (1956-) (élettanász)
Pályázati támogatás:K100151
OTKA
PD101171
OTKA
K101196
OTKA
CNK-77855
Egyéb
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6.

001-es BibID:BIBFORM004739
Első szerző:Starkus, John G.
Cím:Mechanisms of the inhibition of Shaker potassium channels by protons / John G. Starkus, Zoltán Varga, Roland Schönherr, Stefan H. Heinemann
Dátum:2003
ISSN:0031-6768
Megjegyzések:Potassium channels are regulated by protons in various ways and, in most cases, acidification results in potassium current reduction. To elucidate the mechanisms of proton-channel interactions we investigated N-terminally truncated Shaker potassium channels (Kv1 channels) expressed in Xenopus oocytes, varying pH at the intracellular and the extracellular face of the membrane. Intracellular acidification resulted in rapid and reversible channel block. The block was half-maximal at pH 6.48, thus even physiological excursions of intracellular pH will have an impact on K+ current. The block displayed only very weak voltage dependence and C-type inactivation and activation were not affected. Extracellular acidification (up to pH 4) did not block the channel, indicating that protons are effectively excluded from the selectivity filter. Channel current, however, was reduced greatly due to marked acceleration of C-type inactivation at low pH. In contrast, inactivation was not affected in the T449V mutant channel, in which C-type inactivation is impaired. The pH effect on inactivation of the wild-type channel had an apparent pK of 4.7, suggesting that protonation of extracellular acidic residues in Kv channels makes them subject to pH regulation
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animals
Comparative Study
drug effects
Female
Ion Channel Gating
Oocytes
pharmacology
physiology
Potassium
Potassium Channel Blockers
Potassium Channels
Protons
Research
Shaker Superfamily of Potassium Channels
Support
Xenopus
Megjelenés:Pflügers Archiv. - 447 : 1 (2003), p. 44-54. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Schönherr, Roland Heinemann, Stefan H.
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7.

001-es BibID:BIBFORM043347
035-os BibID:PMID:23553419
Első szerző:Szilágyi Orsolya (molekuláris biológus, biokémikus)
Cím:The role of PSD-95 in the rearrangement of Kv1.3 channels to the immunological synapse / Orsolya Szilágyi, Anita Boratkó, György Panyi, Péter Hajdu
Dátum:2013
ISSN:0031-6768
Megjegyzések:Establishment of the immunological synapse (IS) between T lymphocytes and antigen-presenting cells is a key step in the adaptive immune response. Several proteins accumulate in the IS, such as the Kv1.3 potassium channel; however, the mechanism of this translocation is unknown. PSD-95 and SAP97 are adaptor proteins that regulate the polarized cell surface expression and localization of Kv1 channels in neurons. We investigated whether these proteins affect the redistribution of Kv1.3 into the IS in non-excitable human T cells. We show here that PSD-95 and SAP97 are expressed in Jurkat and interact with the C terminus of Kv1.3. Disruption of the interaction between PSD-95 or SAP97 and Kv1.3 in Jurkat was realized by the expression of a C-terminal truncated Kv1.3, which lacks the binding domain for these proteins, or by the knockdown of the expression of PSD-95 or SAP97 using specific shRNA. Expression of the truncated Kv1.3 or knockdown of PSD-95, but not the knockdown of SAP97, inhibited the recruitment of Kv1.3 into the IS; the fraction of cells showing polarized Kv1.3 expression upon engagement in an IS was significantly lower than in control cells expressing the full-length Kv1.3, and the rearrangement of Kv1.3 did not show time dependence. In contrast, Jurkat cells expressing the full-length channel showed marked time dependence in the recruitment into the IS peaking at 1 min after the conjugation of the cells. These results demonstrate that PSD-95 participates in the targeting of Kv1.3 into the IS, implying its important role in human T-cell activation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Doktori iskola
Megjelenés:Pflugers Archiv-European Journal of Physiology. - 465 : 9 (2013), p. 1341-1353. -
További szerzők:Boratkó Anita (1985-) (biokémikus, molekuláris biológus) Panyi György (1966-) (biofizikus) Hajdu Péter (1975-) (biofizikus)
Pályázati támogatás:TÁMOP-4.2.2-A-11/1/KONV-2012-0025
TÁMOP
TÁMOP-4.2.2/B-10/1-2010-0024
TÁMOP
Molekuláris Orvostudomány Doktori Iskola
TÁMOP-4.2.2-08/1-2008-0019
TÁMOP
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8.

001-es BibID:BIBFORM005104
Első szerző:Szűcs Attila (fül-orr-gégész)
Cím:Differential expression of potassium currents in Deiters cells of the guinea pig cochlea / Attila Szűcs, Sándor Somodi, Tamás J. Batta, Andrea Tóth, Gyula P. Szigeti, László Csernoch, György Panyi, István Sziklai
Dátum:2006
ISSN:0031-6768
Megjegyzések:Among the supporting cells, Deiters cells are in intimate contact with outer hair cells (OHCs) in the inner ear. The aim of this study was to characterize the outward rectifying K+ current of Deiters cells in conjunction with cellular morphological characteristics. In the majority of cells, the K+ current had a biphasic inactivation kinetics (tau1 and tau2 were 2,735+/-90 (n=77) and 160+/-14 ms (n=72), respectively). The rapidly inactivating current component was more sensitive to Charybdotoxin (ChTx, 10 nM) block whereas the slowly inactivating current could be blocked more efficiently by tetraethylammonium (1 mM). All these point toward the existence of two distinct potassium channel types in these cells. Deiters cells attached to shorter OHCs had more voluminous, whereas those attached to longer OHCs had lanky cell bodies. The inactivation kinetics was slower in cells having corpulent cell bodies due to the increased proportion of the slowly inactivating current component (0.736+/-0.033, n=27) as compared to the one determined for lanky cells (0.522+/-0.023, n=36). The average peak K+ current was higher in Deiters cells connected to OHCs (5,417+/-541 pA, n=40) than in isolated ones (3,527+/-410, n=37). Deiters cells having different cell shapes and showing different K+ channel expression may contribute to the active mechanism of the cochlea to various degrees.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animals
Cells
Charybdotoxin
cytology
Guinea Pigs
Hungary
Kinetics
metabolism
Organ of Corti
Patch-Clamp Techniques
Potassium
Potassium Channel Blockers
Potassium Channels
Research
Support
Tetraethylammonium
Megjelenés:Pflügers Archiv. - 452 : 3 (2006), p. 332-341. -
További szerzők:Somodi Sándor (1977-) (belgyógyász) Batta József Tamás (1970-) (fül-orr-gégész) Tóth Andrea (1973-) (fül-orr-gégész) Szigeti Gyula (1969-) (élettanász, elektrofiziológus) Csernoch László (1961-) (élettanász) Panyi György (1966-) (biofizikus) Sziklai István (1954-) (fül-orr-gégész)
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