CCL

Összesen 2 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM015796
Első szerző:Bálint Géza
Cím:Ex vivo soft-laser treatment inhibits the synovial expression of vimentin and {alpha}-enolase, potential autoantigens in rheumatoid arthritis / Géza Bálint, Klára Barabás, Zsuzsanna Zeitler, József Bakos, Katalin A. Kékesi, Ákos Pethes, Erzsébet Nagy, Tamás Lakatos, Péter V. Bálint, Zoltán Szekanecz
Dátum:2011
ISSN:1538-6724 (Electronic)
Megjegyzések:Soft-laser therapy has been used to treat rheumatic diseases for decades. The major effects of laser treatment may be dependent not on thermal mechanisms but rather on cellular, photochemical mechanisms. However, the exact cellular and molecular mechanisms of action have not been elucidated. Objective The aim of this study was to investigate the ex vivo effects of low-level laser treatment (with physical parameters similar to those applied previously) on protein expression in the synovial membrane in rheumatoid arthritis (RA). Design Synovial tissues were laser irradiated, and protein expression was analyzed. METHODS:/b> Synovial membrane samples obtained from 5 people who had RA and were undergoing knee surgery were irradiated with a near-infrared diode laser at a dose of 25 J/cm(2) (a dose used in clinical practice). Untreated synovial membrane samples obtained from the same people served as controls. Synovial protein expression was assessed with 2-dimensional polyacrylamide gel electrophoresis followed by mass spectrometry. RESULTS: /b> The expression of 12 proteins after laser irradiation was different from that in untreated controls. Laser treatment resulted in the decreased expression of alpha-enolase in 2 samples and of vimentin and precursors of haptoglobin and complement component 3 in 4 samples. The expression of other proteins, including 70-kDa heat shock protein, 96-kDa heat shock protein, lumican, osteoglycin, and ferritin, increased after laser therapy. Limitations The relatively small sample size was a limitation of the study. CONCLUSIONS:/b> Laser irradiation (with physical parameters similar to those used previously) resulted in decreases in both alpha-enolase and vimentin expression in the synovial membrane in RA. Both proteins have been considered to be important autoantigens that are readily citrullinated and drive autoimmunity in RA. Other proteins that are expressed differently also may be implicated in the pathogenesis of RA. Our results raise the possibility that low-level laser treatment of joints affected with RA may be effective, at least in part, by suppressing the expression of autoantigens. Further studies are needed.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Physical Therapy. -
További szerzők:Barabás Klára Zeitler Zsuzsanna Bakos József (gyógyszerész) Kékesi Katalin Adrienna Pethes Ákos Nagy Erzsébet (orvos, Budapest) Lakatos Tamás Bálint Péter V. Szekanecz Zoltán (1964-) (reumatológus, belgyógyász, immunológus)
Internet cím:DOI
elektronikus változat
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM041924
Első szerző:Gomez Izabella (orvos)
Cím:In Vivo Calcium and Phosphate Iontophoresis for the Topical Treatment of Osteoporosis / Gomez I., Szabó A., Pap L. Jr., Pap L., Boda K., Szekanecz Z.
Dátum:2012
ISSN:0031-9023
Megjegyzések:Background In addition to systemic treatment, osteoporosis may be treated topically by incorporating calcium and phosphate into the bone.Objective This article describes the use of a recently developed, novel iontophoretic apparatus suitable for local ion delivery into bones. In this study, in vivo experiments were performed to compare the effects of local electrotherapy and those of systemic hormone replacement on bone.Design In this study, local iontophoresis was carried out in ovariectomized and control rats. Bone density, biomechanical, and elemental studies were performed.Methods Forty 12-week-old Sprague-Dawley rats received an ovariectomy (OVX) or were sham-operated (sham). Twenty-one weeks later, tibias of subgroups of sham-operated and OVX animals were subjected to serial local iontophoresis (IOP) treatments, received systemic subcutaneous 17?-estradiol (E2), or were treated with the combination of IOP and E2. Changes in bone density were detected by quantitative ultrasound densitometry and expressed as amplitude-dependent speed of sound (AD-SoS). Biomechanical studies and elemental analysis were performed at the end of the experiments.Results Osteopenia developed 21 weeks after OVX in the proximal tibial regions; the mean difference estimate (95% confidence intervals) of AD-SoS values between the sham-operated and OVX animals was 188.7 (140.4?237.1). Serial iontophoretic treatment resulted in an increase in bone density in both sham-operated and OVX animals (sham+IOP versus sham: 121.4 [73.01?169.7]; OVX+IOP versus OVX: 241.6 [193.2?289.9]). Similar changes in AD-SoS were detected after 17?-estradiol (E2) treatment; however, even greater changes occurred after OVX+E2+IOP versus OVX+E2 (123.4 [75.1?171.8]). Similar improvements also were evident regarding the biomechanical features of the tibias.Limitations A limitation of this study was the relatively small number of rats.Conclusions The efficacy of local IOP using calcium- and phosphate-donating microparticles is comparable to that of estrogen therapy as evidenced by steadily increasing bone density, restoration of the calcium and phosphate balance, and improvement in the biomechanical properties of the bone.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Physical Therapy. - 92 : 2 (2012), p. 289-297. -
További szerzők:Szabó Andrea Pap Lajos (jr.) Pap Lajos (1929-) (vegyész, analitikus) Boda Katalin Szekanecz Zoltán (1964-) (reumatológus, belgyógyász, immunológus)
Pályázati támogatás:T 048541
OTKA
TÁMOP-4.2.2-08/1-2008-0013
TÁMOP
TÁMOP-4.2.1/B-09/1/KONV-2010-0005
TÁMOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1