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001-es BibID:BIBFORM059770
Első szerző:Kalló Gergő (molekuláris biológus)
Cím:Relative quantification of human [béta]-defensins by a proteomics approach based on selected reaction monitoring / Gergő Kalló, Arunima Chatterjee, Márta Tóth, Éva Rajnavölgyi, Adrienne Csutak, József Tőzsér, Éva Csősz
Dátum:2015
ISSN:0951-4198
Megjegyzések:RATIONALE: A targeted proteomics method based on selected reaction monitoring (SRM) is a relevant approach for theanalysis of multiple analytes in biological samples. Defensins are phylogenetically conserved small antimicrobialpeptides contributing to innate host defense and exhibiting low immunogenicity, resistance to proteolysis and a broadrange of antimicrobial activities. The goal of the present study was to develop and optimize SRM-based targetedproteomics methods for the detection of human [béta]-defensins 1?4 in various biological fluids.METHODS: An SRM-based targeted proteomics method was developed and validated for the detection of human?-defensins 1?4. The supernatants of resting and IL-1?-stimulated Caco2, HT-29 and SW-1116 colonic epithelial cells(CEC), cell lysates of CECs and tear samples of human healthy individuals were analyzed and the feasibility of thedeveloped method was validated by ELISA and dot-blot analysis complemented by RT-qPCR.RESULTS: Our results demonstrate that the developed SRM method offers an alternative approach for the cost-effectiveand rapid analysis of human [béta]-defensins in samples with biological relevance.CONCLUSIONS: A semi-quantitative targeted mass spectrometry method was developed and validated for the relativequantification of [béta]-defensins 1?4 in cell culture supernatants and body fluid analyses.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
[béta]-defensin
tear
relative quantification
targeted proteomics
SRM
MRM
Megjelenés:Rapid Communications In Mass Spectrometry. - 29 : 18 (2015), p. 1623-1631. -
További szerzők:Chatterjee, Arunima (1981-) (immunológus) Tóth Márta (1986-) (biológus) Rajnavölgyi Éva (1950-) (immunológus) Csutak Adrienne (1971-) (szemész) Tőzsér József (1959-) (molekuláris biológus, biokémikus, vegyész) Csősz Éva (1977-) (biokémikus, molekuláris biológus)
Pályázati támogatás:TÁMOP 4.2.4.A/2-11-1-2012-0001
TÁMOP 4.2.2.A- 11/1/KONV-2012-0045
TÁMOP 4.2.2.A-11/1/KONV-2012-0023
Marie-Curie Cross-Talk program FP7-215553 (2007?2013)
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001-es BibID:BIBFORM036705
Első szerző:Rühl, Ralph (vegyész)
Cím:Method to determine 4-oxo-retinoic acids, retinoic acids and retinol in serum and cell extracts by liquid chromatography/diode-array detection atmospheric pressure chemical ionisation tandem mass spectrometry / Ralph Ruhl
Dátum:2006
Megjegyzések:Retinoic acid isomers, 4-oxo-retinoic acid isomers and retinol are present in the serum of mammals. In this study a high-performance liquid chromatography (HPLC) separation, sample preparation and tandem mass spectrometry (MS/MS) method was established for quick and easy sample preparation and sensitive determination of retinoids such as all-trans-4-oxo-retinoic acid, 13-cis-4-oxo-retinoic acid, 13-cis-retinoic acid, 9-cis-retinoic acid, all-trans-retinoic acid and retinol in serum and cell extracts. Serum samples were simply treated with three times the volume of isopropanol, dried under vacuum, taken up in the HPLC solvent and immediately put into the autosampler for an automated single-run HPLC analysis. With this MS/MS method we were able to detect 7 pg and quantify 20 pg of all-trans-retinoic acid, 4-oxo-all-trans-retinoic acid and retinol directly on-column and were able to determine a concentration as low as 0.2 ng/mL in ethanolic standards and in biological samples. This method allows ultra-sensitive detection, excellent selectivity and a very simple sample preparation to determine retinoic acids, 4-oxo-retinoic acids and retinol in serum and cell extracts for the study of endogenous retinoids.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Rapid Communications in Mass Spectrometry. - 20 : 16 (2006), p. 2497-2504. -
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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