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001-es BibID:BIBFORM105401
035-os BibID:(cikkazonosító)1003709 (WoS)000868652700001 (Scopus)85139183146
Első szerző:Kocsis Beatrix (molekuláris biológus)
Cím:Functional analysis of the bZIP-type transcription factors AtfA and AtfB in Aspergillus nidulans / Beatrix Kocsis, Mi-Kyung Lee, Jae-Hyuk Yu, Tibor Nagy, Lajos Daróczi, Gyula Batta, István Pócsi, Éva Leiter
Dátum:2022
ISSN:1664-302X
Megjegyzések:Transcription factors (TFs) with the basic leucin zipper domain are key elements of the stress response pathways in filamentous fungi. In this study, we functionally characterized the two bZIP type TFs AtfA and AtfB by deletion (Delta) and overexpression (OE) of their encoding genes in all combination: Delta atfA, Delta atfB, Delta atfA Delta atfB, Delta atfAatfBOE, Delta atfBatfAOE, atfAOE, atfBOE and atfAOEatfBOE in Aspergillus nidulans. Based on our previous studies, Delta atfA increased the sensitivity of the fungus to oxidative stress mediated by menadione sodium bisulfite (MSB) and tert-butylhydroperoxide (tBOOH), while Delta atfB was not sensitive to any oxidative stress generating agents, namely MSB, tBOOH and diamide at all. Contrarily, the Delta atfB mutant was sensitive to NaCl, but tolerant to sorbitol. Overexpression of atfB was able to compensate the MSB sensitivity of the Delta atfA mutant. Heavy metal stress elicited by CdCl2 reduced diameter of the atfBOE and atfAOEatfBOE mutant colonies to about 50% of control colony, while the cell wall stress generating agent CongoRed increased the tolerance of the Delta atfA mutant. When we tested the heat stress sensitivity of the asexual spores (conidiospores) of the mutants, we found that conidiospores of Delta atfAatfBOE and Delta atfBatfAOE showed nearly 100% tolerance to heat stress. Asexual development was negatively affected by Delta atfA, while atfAOE and atfAOE coupled with Delta atfB increased the number of conidiospores of the fungus approximately 150% compared to the control. Overexpression of atfB led to a 25% reduction in the number of conidiospores, but increased levels of abaA mRNA and size of conidiospores. Sexual fruiting body (cleistothecium) formation was diminished in the Delta atfA and the Delta atfA Delta atfB mutants, while relatively elevated in the Delta atfB and the Delta atfBatfAOE mutants. Production of the mycotoxin sterigmatocystin (ST) was decreased to undetectable levels in the Delta atfA mutant, yet ST production was restored in the Delta atfA Delta atfB mutant, suggesting that Delta atfB can suppress ST production defect caused by Delta atfA. Levels of ST were also significantly decreased in the Delta atfAatfBOE, Delta atfBatfAOE and atfAOEatfBOE mutants.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
aspergillus nidulans
environmental stress
conidiospore
cleistothecium
sterigmatocystin
Megjelenés:Frontiers in Microbiology. - 13 (2022), p. 1-9. -
További szerzők:Lee Mi-Kyung Yu Jae-Hyuk Nagy Tibor (1988-) (vegyész) Daróczi Lajos (1965-) (fizikus) Batta Gyula (1979-) (biológus) Pócsi István (1961-) (vegyész) Leiter Éva (1976-) (biológus)
Pályázati támogatás:NKFIH
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2.

001-es BibID:BIBFORM081370
035-os BibID:(cikkazonosító)212 (WOS)000371204800001 (Scopus)84962124092
Első szerző:Sipiczki Mátyás (biológus)
Cím:Overwintering of Vineyard Yeasts : Survival of Interacting Yeast Communities in Grapes Mummified on Vines / Matthias Sipiczki
Dátum:2016
ISSN:1664-302X
Megjegyzések:The conversion of grape must into wine involves the development and succession of yeast populations differing in species composition. The initial population is formed by vineyard strains which are washed into the must from the crushed grapes and then completed with yeasts coming from the cellar environment. As the origin and natural habitat of the vineyard yeasts are not fully understood, this study addresses the possibility, that grape yeasts can be preserved in berries left behind on vines at harvest until the spring of the next year. These berries become mummified during the winter on the vines. To investigate whether yeasts can survive in these overwintering grapes, mummified berries were collected in 16 localities in the Tokaj wine region (Hungary-Slovakia) in early March. The collected berries were rehydrated to recover viable yeasts by plating samples onto agar plates. For the detection of minority species which would not be detected by direct plating, an enrichment step repressing the propagation of alcohol-sensitive yeasts was also included in the process. The morphological, physiological, and molecular analysis identified 13 basidiomycetous and 23 ascomycetous species including fermentative yeasts of wine-making relevance among the 3879 isolates. The presence of viable strains of these species demonstrates that the grapes mummified on the vine can serve as a safe reservoir of yeasts, and may contribute to the maintenance of grape-colonizing yeast populations in the vineyard over years, parallel with other vectors and habitats. All basidiomycetous species were known phylloplane yeasts. Three Hanseniaspora species and pigmented Metschnikowia strains were the most frequent ascomycetes. Other fermentative yeasts of wine-making relevance were detected only in the enrichment cultures. Saccharomyces (S. paradoxus, S. cerevisiae, and S. uvarum) were recovered from 13% of the samples. No Candida zemplinina was found. The isolates with Aureobasidium morphology turned out to belong to Aureobasidium subglaciale, Kabatiella microsticta, or Columnosphaeria fagi. The ascomyceteous isolates grew at high concentrations of sugars with Wickerhamomyces anomalus being the most tolerant species. Complex interactions including antagonism (growth inhibition, contact inhibition, competition for nutrients) and synergism (crossfeeding) among the isolates and with Botrytis cinerea shape the composition of the overwintering communities.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
yeasts
grape
Tokaj
molecular taxonomy
diversity
antagonism
wine
Megjelenés:Frontiers in Microbiology. - 7 (2016), p. 1-17. -
Pályázati támogatás:OTKA 81792
OTKA
OTKA 101323
OTKA
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3.

001-es BibID:BIBFORM078529
035-os BibID:(cikkazonosító)3071 (WOS)000453083900001 (Scopus)85058339102
Első szerző:Sipiczki Mátyás (biológus)
Cím:Interspecies hybridisation and genome chimerisation in Saccharomyces : combining of gene pools of species and its biotechnological perspectives / Matthias Sipiczki
Dátum:2018
ISSN:1664-302X
Megjegyzések:Over the last one and a half decade, interspecies hybridisation has gained continuously increasing attention as a breeding technique suitable for transferring of genetic information between Saccharomyces species and mixing of their gene pools without genetic engineering. The hybrids frequently show positive transgressive phenotypes. Segregation of the hybrid genome results in mosaic (chimeric) strains that can outperform both the parents and the hybrids or exhibit novel positive phenotypic properties. Mitotic segregation can take place during the vegetative propagation of the sterile allodiploid hybrid cells. Meiotic segregation becomes possible after genome duplication (tetraploidisation) if it is followed by break-down of sterility. The allotetraploid cells are seemingly fertile because they form viable spores. But because of the autodiploidisation of the meiosis, sterile allodiploid spores are produced and thus the hybrid genome does not segregate (the second sterility barrier). However, malsegregation of MAT-carrying chromosomes in one of the subgenomes during allotetraploid meiosis (loss of MAT heterozygosity) results in fertile alloaneuploid spores. The breakdown of (the second) sterility barrier is followed by the loss of additional chromosomes in rapid succession and recombination between the subgenomes. The process (genome autoreduction in meiosis or GARMe) chimerises the genome and generates strains with chimeric (mosaic) genomes composed of various combinations of the genes of the parental strains. Since one of the subgenomes is preferentially reduced, the outcome is usually a strain having an (almost) complete genome from one parent and only a few genes or mosaics from the genome of the other parent. The fertility of the spores produced during GARMe provides possibilities also for introgressive backcrossing with one or the other parental strain, but genome chimerisation and gene transfer through series of backcrosses always with the same parent is likely to be less efficient than through meiotic or mitotic genome autoreduction. Hybridisation and the evolution of the hybrid genome (resizing and chimerisation) have been exploited in the improvement of industrial strains and applied to the breeding of new strains for specific purposes. Lists of successful projects are shown and certain major trends are discussed.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
interspecies hybridisation
sterility
alloploid
meiosis
genome chimerisation
strain improvement
fermentation
yeast
Megjelenés:Frontiers in Microbiology. - 9 (2018), p. 1-20. -
Pályázati támogatás:K-124417
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4.

001-es BibID:BIBFORM074051
035-os BibID:(cikkazonosító)1193 (WOS)000434951800001 (Scopus)85048341000
Első szerző:Sipiczki Mátyás (biológus)
Cím:Birth-and-Death Evolution and Reticulation of ITS Segments of Metschnikowia andauensis and Metschnikowia fructicola rDNA Repeats / Sipiczki Matthias, Horvath Eniko, Pfliegler Walter P.
Dátum:2018
ISSN:1664-302X
Megjegyzések:The internal transcribed spacer (ITS) region (ITS1, 5.8S rDNA, and ITS2) separates the genes coding for the SSU 18S and the LSU 26S genes in the rDNA units which are organized into long tandem arrays in the overwhelming majority of fungi. As members of a multigenic family, these units are subject of concerted evolution, which homogenizes their sequences. Exceptions have been observed in certain groups of plants and in a few fungal species. In our previous study we described exceptionally high degree of sequence diversity in the D1/D2 domains of two pulcherrimin-producing Metschnikowia (Saccharomycotina) species which appeared to evolve by reticulation. The major goals of this study were the examination of the diversity of the ITS segments and their evolution. We show that the ITS sequences of these species are not homogenized either, differ from each other by up to 38 substitutions and indels which have dramatic effects on the predicted secondary structures of the transcripts. The high intragenomic diversity makes the D1/D2 domains and the ITS spacers unsuitable for barcoding of these species and therefore the taxonomic position of strains previously assigned to them needs revision. By analyzing the genome sequence of the M. fructicola type strain, we also show that the rDNA of this species is fragmented, contains pseudogenes and thus evolves by the birth-and-death mechanism rather than by homogenisation, which is unusual in yeasts. The results of the network analysis of the sequences further indicate that the ITS regions are also involved in reticulation. M. andauensis and M. fructicola can form interspecies hybrids and their hybrids segregate, providing thus possibilities for reticulation of the rDNA repeats.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
structure prediction
yeast
ribosomal RNA
ITS
evolution
pseudogene
reticulation
hybridisation
Megjelenés:Frontiers in Microbiology. - 9 (2018), p. 1-16. -
További szerzők:Horváth Enikő (1987-) (biológus) Pfliegler Valter Péter (1986-) (molekuláris biológus)
Pályázati támogatás:K-124417
Egyéb
TÁMOP-4.2.4.A/2-11/1-2012-0001
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5.

001-es BibID:BIBFORM102694
035-os BibID:(cikkazonosító)838 (WOS)000536917600001 (Scopus)85085094425
Első szerző:Szabó Adrienn (biológus)
Cím:Diversity and Postzygotic Evolution of the Mitochondrial Genome in Hybrids of Saccharomyces Species Isolated by Double Sterility Barrier / Szabó Adrienn, Antunovics Zsuzsa, Karanyicz Edina, Sipiczki Matthias
Dátum:2020
ISSN:1664-302X
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
yeast hybrids
Megjelenés:Frontiers in Microbiology. - 11 (2020), p. 1-22. -
További szerzők:Antunovics Zsuzsa (1978-) (molekuláris biológus, genetikus) Karanyicz Edina Sipiczki Mátyás (1948-) (biológus)
Pályázati támogatás:NKFIH-K-124417
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