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001-es BibID:	BIBFORM080015
035-os BibID:	(cikkazonosító)126298
Első szerző:	Imre Alexandra (1992-)
Cím:	A new, rapid multiplex PCR method identifies frequent probiotic origin among clinical Saccharomyces isolates / Imre Alexandra, Rácz Hanna Viktória, Antunovics Zsuzsa, Rádai Zoltán, Kovács Renátó, Lopandic Ksenija, Pócsi István, Pfliegler Walter P.
Dátum:	2019
ISSN:	0944-5013
Megjegyzések:	An increasing number of infections originating from probiotic use are reported worldwide, with the majority of such cases caused by the yeast Saccharomyces 'boulardii', a subtype of S. cerevisiae. Reliably linking infectious cases to probiotic products requires unequivocal genotyping data, however, these techniques are often timeconsuming and difficult to implement in routine diagnostics. This leads to a widespread lack of genetic data regarding the origin of Saccharomyces infections. We propose a quick and reliable PCR-based protocol for the identification of S. 'boulardii' based on a combined analysis of interdelta fingerprinting and microsatellite typing. By applying various typing methods and our proposed method to the clinical yeast collection of a Hungarian hospital we show that probiotic origin is common among clinical Saccharomyces, and that the new multiplex method enables rapid and unequivocal identification of probiotic yeast infections. This method can be applied for the identification of yeast infection sources, helping decisions on probiotic use.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Probiotics Saccharomyces boulardii Fungemia Mycoses Mycological typing techniques
Megjelenés:	Microbiological Research. - 227 (2019), p. 1-7. -
További szerzők:	Rácz Hanna Viktória (1994-) (biológus) Antunovics Zsuzsa (1978-) (molekuláris biológus, genetikus) Rádai Zoltán (1991-) (biológus) Kovács Renátó László (1987-) (molekuláris biológus) Lopandic, Ksenija Pócsi István (1961-) (vegyész) Pfliegler Valter Péter (1986-) (molekuláris biológus)
Pályázati támogatás:	ÚNKP-18-3-I-DE-4 Egyéb AÖU 98öu7 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM055087
Első szerző:	Pfliegler Valter Péter (molekuláris biológus)
Cím:	Diversity of Candida zemplinina isolates inferred from RAPD, micro/minisatellite and physiological analysis / Walter P. Pfliegler, Enikő Horváth, Zoltán Kállai, Matthias Sipiczki
Dátum:	2014
ISSN:	0944-5013
Megjegyzések:	Among non-Saccharomyces wine yeasts, Candida zemlpinina is one of the frequently isolated and oenologically important species. It is mostly known from European winemaking areas and it has become one of the key species of non-Saccharomyces wine yeasts to study. Investigating the diversity of C. zemplinina isolates is important for a deeper understanding of the non-Saccharomyces wine yeasts and for the yeast starter industry, as numerous researches have pointed to the potential use of this species in winemaking. For assessing the biodiversity of a larger number of strains, RAPD and micro/minisatellite PCR is often the method of choice, however, this technique is often unstandardized. Whereas some laboratories use these methods for species identifications, others apply RAPD primers for determining intraspecies diversity. In this study, we have tested 5 different RAPD and micro/minisatellite primers on strains of C. zemplinina isolated from different locations. We show that after a rigorous PCR-optimization aimed at reproducibility and comparability of band patterns with these PCR-reactions, diversity of different strains from a wide range of geographic locations is relatively low. The analysis of several oenologically important physiological traits of the strains showed a relatively low level of diversity as well. We also demonstrate that the intraspecific diversity of C. zemplinina observable with different techniques (RAPD, micro/minisatellite or physiological analysis) may be fairly different and not necessarily comparable.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Microbiological Research. - 169 : 5-6 (2014), p. 402-410. -
További szerzők:	Horváth Enikő (1987-) (biológus) Kállai Zoltán (1986-) (biológus) Sipiczki Mátyás (1948-) (biológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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