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001-es BibID:BIBFORM059158
Első szerző:Ferenczi Szilamér (biológus)
Cím:A New Ochratoxin A Biodegradation Strategy Using Cupriavidus basilensis Őr16 Strain / Szilamér Ferenczi, Mátyás Cserháti, Csilla Krifaton, Sándor Szoboszlay, József Kukolya, Zsuzsanna Szőke, Balázs Kőszegi, Mihály Albert, Teréz Barna, Miklós Mézes, Krisztina J. Kovács, Balázs Kriszt
Dátum:2014
ISSN:1932-6203
Megjegyzések:Ochratoxin-A (OTA) is a mycotoxin with possibly carcinogenic and nephrotoxic effects in humans and animals. OTA is often found as a contaminant in agricultural commodities. The aim of the present work was to evaluate OTA-degrading and detoxifying potential of Cupriavidus basilensis ŐR16 strain. In vivo administration of OTA in CD1 male mice (1 or 10 mg/kg body weight for 72 hours or 0.5 mg/kg body weight for 21 days) resulted in significant elevation of OTA levels in the blood, histopathological alterations- and transcriptional changes in OTA-dependent genes (annexinA2, clusterin, sulphotransferase and gadd45 and gadd153) in the renal cortex. These OTA-induced changes were not seen in animals that have been treated with culture supernatants in which OTA was incubated with Cupriavidus basilensis ŐR16 strain for 5 days. HPLC and ELISA methods identified ochratoxin ? as the major metabolite of OTA in Cupriavidus basilensis ŐR16 cultures, which is not toxic in vivo. This study has demonstrated that Cupriavidus basilensis ŐR16 efficiently degrade OTA without producing toxic adventitious metabolites.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
body weight
biodegradation
kidneys
High performance liquid chromatography
spleen
toxicity
blood plasma
detoxification
Megjelenés:Plos One. - 9 : 10 (2014), p. e109817-. -
További szerzők:Cserháti Mátyás Krifaton Csilla Szoboszlay Sándor Kukolya József Szőke Zsuzsanna Kőszegi Balázs Albert Mihály Barna Teréz (1963-) (vegyész) Mézes Miklós Kovács Krisztina J. Kriszt Balázs
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2.

001-es BibID:BIBFORM059253
Első szerző:Kriszt Rókus
Cím:A New Zearalenone Biodegradation Strategy Using Non-Pathogenic Rhodococcus pyridinivorans K408 Strain / Rókus Kriszt, Csilla Krifaton, Sándor Szoboszlay, Mátyás Cserháti, Balázs Kriszt, József Kukolya, Árpád Czéh, Szilvia Fehér-Tóth, Lívia Török, Zsuzsanna Szőke, Krisztina J. Kovács, Teréz Barna, Szilamér Ferenczi
Dátum:2012
ISSN:1932-6203
Megjegyzések:Zearalenone (hereafter referred to as ZEA) is a nonsteroidal estrogenic mycotoxin produced by several Fusarium spp. on cereal grains. ZEA is one of the most hazardous natural endocrine disrupting chemicals (EDC) which induces hyper estrogenic responses in mammals. This can result in reproductive disorders in farm animals as well as in humans. Consequently, detoxification strategies for contaminated crops are crucial for food safety. In this study we have developed a bacterial based detoxification system using a non-pathogen Rhodococcus pyridinivorans K408 strain. Following 5 days treatment of ZEA with R. pyridinivorans K408 strain HPLC analyses showed an 87.21% ZEA-degradation efficiency of the bacterial enzyme systems. In another approach, the strain biotransformation ability has also been confirmed by a bioluminescent version of the yeast estrogen screening system (BLYES), which detected an 81.75% of biodegradability of ZEA, in a good agreement with the chemical analyses. Furthermore, the capacity of R. pyridinivorans to eliminate the estrogenic effects of ZEA was tested by using an immature uterotrophic assay. Prepubertal female rats were treated with vehicle (olive oil), 17?-estradiol, ZEA (0.1-1-5-10 mg/kg body weight) and LB broth containing 500 mg/l ZEA that has already been incubated with or without Rhodococcus pyridinivorans K408 strain. Uterine weights were measured and the mRNA level changes relating to apelin, aquaporin 5, complement component 2, and calbindin-3 genes were measured by qRT-PCR. These genes represent the major pathways that are affected by estromimetic compounds. Zearalenone feeding significantly increased the uterus weight in a dose dependent manner and at the same time upregulated complement component 2 and calbindin-3 expression as well as decreased apelin and aquaporin 5 mRNA levels comparable to that seen in 17?-estradiol exposed rats. In contrast, LB broth in which ZEA was incubated with Rhodococcus pyridinivorans K408 prior to the feeding did not display any estrogenic effect neither on uterine weight nor on the expression of estrogen-regulated genes. Consequently, the identification of Rhodococcus pyridinivorans K408 strain in ZEA biodegradation proved to be a very efficient biological tool that is able to eliminate the complete estrogenic effects of ZEA. It is also remarkable that this biotransformation pathway of ZEA did not result in any residual estrogenic effects.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
zea
rhodococcus
estrogens
Biodegradation
Metabolites
yeast estrogen screen
Uterus
gene expression
Megjelenés:Plos One. - 7 : 9 (2012), p. e43608-. -
További szerzők:Krifaton Csilla Szoboszlay Sándor Cserháti Mátyás Kriszt Balázs Kukolya József Czéh Árpád Fehér-Tóth Szilvia Török Lívia Szőke Zsuzsanna Kovács Krisztina J. Barna Teréz (1963-) (vegyész) Ferenczi Szilamér (1977-) (biológus)
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3.

001-es BibID:BIBFORM055088
Első szerző:Sipiczki Mátyás (biológus)
Cím:Metschnikowia Species Share a Pool of Diverse rRNA Genes Differing in Regions That Determine Hairpin-Loop Structures and Evolve by Reticulation / Matthias Sipiczki, Walter P. Pfliegler, Imre J. Holb
Dátum:2013
ISSN:1932-6203
Megjegyzések:Modern taxonomy of yeasts is mainly based on phylogenetic analysis of conserved DNA and protein sequences. By far the most frequently used sequences are those of the repeats of the chromosomal rDNA array. It is generally accepted that the rDNA repeats of a genome have identical sequences due to the phenomenon of sequence homogenisation and can thus be used for identification and barcoding of species. Here we show that the rDNA arrays of the type strains of Metschnikowia andauensis and M. fructicola are not homogenised. Both have arrays consisting of diverse repeats that differ from each other in the D1/D2 domains by up to 18 and 25 substitutions. The variable sites are concentrated in two regions that correspond to back-folding stretches of hairpin loops in the predicted secondary structure of the RNA molecules. The substitutions do not alter significantly the overall hairpin-loop structure due to wobble base pairing at sites of C-T transitions and compensatory mutations in the complementary strand of the hairpin stem. The phylogenetic and network analyses of the cloned sequences revealed that the repeats had not evolved in a vertical tree-like way but reticulation might have shaped the rDNA arrays of both strains. The neighbour-net analysis of all cloned sequences of the type strains and the database sequences of different strains further showed that these species share a continuous pool of diverse repeats that appear to evolve by reticulate evolution.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Plos One. - 8 : 6 (2013), p. e67384-. -
További szerzők:Pfliegler Valter Péter (1986-) (molekuláris biológus) Holb Imre (1973-) (agrármérnök)
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4.

001-es BibID:BIBFORM068817
035-os BibID:(cikkazonosító)e0155769 (WOS)000376881700029 (Scopus)84971201028
Első szerző:Tóth Ákos
Cím:Cloning, Expression and Biochemical Characterization of Endomannanases from Thermobifida Species Isolated from Different Niches / Ákos Tóth, Terézia Barna, Erna Szabó, Rita Elek, Ágnes Hubert, István Nagy, István Nagy, Balázs Kriszt, András Táncsics, József Kukolya
Dátum:2016
ISSN:1932-6203
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
endomannanase
Megjelenés:Plos One. - 11 : 5 (2016), p. e0155769-. -
További szerzők:Barna Teréz (1963-) (vegyész) Szabó Erna (1990-) (Biológus) Elek Rita Hubert Ágnes Nagy István Kriszt Balázs Táncsics András Kukolya József
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