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001-es BibID:BIBFORM105029
035-os BibID:(WoS)000497251800056 (Scopus)85073628804 (cikkazonosító)104627
Első szerző:Láng Orsolya
Cím:Cell physiological effects of glass ionomer cements on fibroblast cells / Orsolya Lang, Lszlo Kohidai, Zsofia Kohidai, Csaba Dobo-Nagy, Krisztian B. Csomo, Mira Lajko, Miklos Mozes, Sandor Keki, Gyorgy Deak, Kun V. Tian, Veronika Gresz
Dátum:2019
ISSN:0887-2333
Megjegyzések:The cytotoxicity of glass ionomer cements (GICs) was investigated using a novel, cost-effective, easy-to-perform and standardized test. GIC rings were made using in-house designed, custom-made moulds under sterile conditions; 10 with Fuji Equia and 10 with Fuji Triage capsules, placed in direct contact with primary human gingival fibroblasts (HGF) and immortalized human fibroblasts (HFF1). On day 1, 4, 14 and 21, an AlamarBlue (resazurin) assay was completed towards determining the effects of the GICs on metabolic activities of the cells, whilst cell morphology was examined by light microscopy. The influence of the compounds released from the GIC rings on cell physiological effects (viability, proliferation and adhesion) during 24?h incubation was further investigated by impedimetry. Result trends obtained from this battery of techniques were complementary. At 100?v/v% concentration, the released compounds from Equia were strongly cytotoxic, while at lower concentration (0, 4, 20?v/v%) they were not cytotoxic. In contrast, Triage elicited only slightly transient cytotoxicity. The method proposed has been proved as being efficient, reliable and reproducible and may be useful in quick testing of the cytotoxicity of similar biomaterials by using an immortalized cell line.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Cytotoxicity
Fibroblast
Glass ionomer cement
Histology
Biomaterial
Megjelenés:Toxicology In Vitro. - 61 (2019), p. 104627. -
További szerzők:Kőhidai László Kőhidai Zsófia Dobó Nagy Csaba (1961-) (fogszakorvos) Csomó Krisztián Benedek Lajkó Mira Mózes Miklós Kéki Sándor (1964-) (polimer kémikus) Deák György (1954-) (polimer kémikus) Tian, Kun V. Gresz Veronika
Pályázati támogatás:EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
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001-es BibID:BIBFORM073109
Első szerző:Nagy Zsolt
Cím:MICAN, a new fluorophore for vital and non-vital staining of human cells / Nagy Zsolt, Nagy Miklós, Kiss Alexandra, Rácz Dávid, Barna Beatrix, Könczöl Péter, Bankó Csaba, Bacsó Zsolt, Kéki Sándor, Banfalvi Gaspar, Szemán-Nagy Gábor
Dátum:2018
ISSN:0887-2333
Megjegyzések:Fluorescence time-lapse microscopy is in connection with the invasive properties of fluorochrome applied, andwith the toxicity of the excitation energy and wavelength of the dye itself. Experiments with the newly synthesizedfluorescent dye 1-N-methylamino-5-isocyanonaphthalene (MICAN) served to test its cytotoxicity onhuman HaCaT keratinocyte cell cultures. Experiments related to staining capability were performed with paraformaldehyde(PFA) fixed cells and observed with fluorescence microscope. It was assumed that the fluorophore1-amino-5-isocyanonaphthalene (ICAN) and especially its N-methylamino derivative MICAN, containingcondensed aromatic rings could serve as a nonselective fluorescent dye capable to stain cellular structures offixed, living, damaged and dead cells. This notion was confirmed by the MICAN staining of cytoplasmic proteinsprimarily rough endoplasmic reticulum (RER), smooth endoplasmic reticulum (SEM) and less efficiently nuclearproteins suggesting the involvement of staining of subcellular structures involved in protein synthesis. MICANwas not only well tolerated by living cells but turned out to be a strong heterochromatin and RER staining agent.This led to the development of a MICAN staining protocol for native and living samples. Relative to otherfluorescent dyes, MICAN is not only useful but also cost-effective. Toxicology tests were performed using 30, 10,5, 0.5 ?g/ml MICAN concentrations. Time-lapse videomicroscopy at near-infrared (NIR) illumination has beenused for the examination of MICAN effect on cell division. It was found that MICAN as a vital stain had nosignificant harmful effect on HaCaT cells. MICAN turned out to be a non-toxic, highly quantum-efficient vitalstain with minimal, or no photobleaching, and can be applied to co-stain with propidium-iodide due the strongspectral separation.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Fluorescence imaging
Solvatochromism
Vital staining
Isocyanide
MICAN
Megjelenés:Toxicology In Vitro. - 48 (2018), p. 137-145. -
További szerzők:Nagy Miklós (1976-) (vegyész) Kiss Alexandra (1994-) (gyógyszer-biotechnológus) Rácz Dávid (1987-) (vegyész) Barna Beatrix Könczöl Péter (1972-) (nemesítő) Bankó Csaba (1989-) (molekuláris biológus) Bacsó Zsolt (1963-) (biofizikus) Kéki Sándor (1964-) (polimer kémikus) Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész) Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus)
Pályázati támogatás:GINOP-2.3.2-15-2016-00041
GINOP
116465
OTKA
János Bolyai Research Scholarship of the Hungarian Academy of Sciences
MTA
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